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Rabbit Recombinant Monoclonal PSME3 antibody. Suitable for IHC-P and reacts with Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSME3 antibody [EPR24504-11] (AB282576), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSME3 antibody [EPR24504-11] (AB282576), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSME3 antibody [EPR24504-11] (AB282576), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSME3 antibody [EPR24504-11] (AB282576), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBIHC-PFlow Cyt (Intra)
Human
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
-
Notes

-

Species
Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Subunit of the 11S REG-gamma (also called PA28-gamma) proteasome regulator, a doughnut-shaped homoheptamer which associates with the proteasome. 11S REG-gamma activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. Facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. May also be involved in cell cycle regulation. Mediates CCAR2 and CHEK2-dependent SIRT1 inhibition (PubMed:25361978).

Alternative names

Proteasome activator complex subunit 3, 11S regulator complex subunit gamma, Activator of multicatalytic protease subunit 3, Ki nuclear autoantigen, Proteasome activator 28 subunit gamma, REG-gamma, PA28g, PA28gamma, PSME3

Recommended products

Rabbit Recombinant Monoclonal PSME3 antibody. Suitable for IHC-P and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR24504-11
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The PSME3 protein also known as PA28γ or REGγ is mechanically an essential proteasome activator that facilitates the degradation of ubiquitin-independent substrates. It has a molecular weight of approximately 29 kDa. PSME3 is mainly found in the nucleus and shows strong expression in various tissues including liver kidney and heart. The protein operates by binding to the 20S proteasome core and enhances the specificity of proteolytic activity.

Biological function summary

PSME3 regulates cell cycle progression and apoptosis. It is part of a larger multiprotein complex with the 20S proteasome core forming an important component in protein homeostasis. PSME3’s interaction with the proteasome core significantly reduces the accumulation of certain regulatory proteins by promoting their degradation. This function affects several cellular processes such as cell proliferation and DNA repair aiding the maintenance of cellular function and integrity.

Pathways

PSME3 plays a critical role in mediating the ubiquitin-proteasome pathway and the MDM2-p53 pathway. In the ubiquitin-proteasome pathway it assists in the controlled turnover of intracellular proteins which is pivotal for maintaining cellular protein quality. In the MDM2-p53 pathway it affects the stability of the p53 protein by enhancing the degradation of both MDM2 and p53 therefore modulating apoptosis and growth arrest in cells under stress or DNA damage conditions.

Associated diseases and disorders

Disruptions in PSME3 function correlate with cancer and neurodegenerative disorders. Overexpression of PSME3 is often linked to oncogenesis due to its role in controlling the degradation of tumor suppressor proteins like p53. This protein is also implicated in Parkinson’s disease where its interaction with pathogenic proteins suggests it might contribute to the accumulation of misfolded proteins. PSME3 along with its pathways and related proteins offers potential as a target for therapeutic intervention in these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSME3 antibody [EPR24504-11] (ab282576), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSME3 antibody [EPR24504-11] (ab282576)

    Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labelling PSME3 with ab282576 at 1/2000 (0.266 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining in human colon cancer.The section was incubated with ab282576 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSME3 antibody [EPR24504-11] (ab282576), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSME3 antibody [EPR24504-11] (ab282576)

    Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labelling PSME3 with ab282576 at 1/2000 (0.266 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining in human lung cancer (PMID: 28605165). The section was incubated with ab282576 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSME3 antibody [EPR24504-11] (ab282576), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSME3 antibody [EPR24504-11] (ab282576)

    Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissue labelling PSME3 with ab282576 at 1/2000 (0.266 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining in human bladder cancer. The section was incubated with ab282576 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSME3 antibody [EPR24504-11] (ab282576), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSME3 antibody [EPR24504-11] (ab282576)

    Immunohistochemical analysis of paraffin-embedded Parental HEK293T (A) PSME3 KO HEK293T (B) tissue labelling PSME3 with ab282576 at 1/2000 (0.266 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Mainly nuclear staining in Parental HEK293T (A) and nearly no staining in PSME3 KO HEK293T (B). The section was incubated with ab282576 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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