AB140497

Anti-PSMF1 antibody

Name: Anti-PSMF1 antibody (ab140497) | Abcam
Brand: Abcam Limited
SKU: AB140497
Rating: 4 (1 reviews)

(1 Review)

(2 Publications)

Rabbit Polyclonal PSMF1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human Proteasome inhibitor PI31 subunit aa 200 to C-terminus.

View Alternative Names

Proteasome inhibitor PI31 subunit, hPI31, PSMF1

2 Images

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Immunoprecipitation - Anti-PSMF1 antibody (AB140497)

ab140497 at 1 μg/ml detecting PSMF1 in 293T whole cell lysate by WB following IP.

Lane 1 : ab140497 at 6μg/mg of lysate
Lane 2 : control IgG.

In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.

Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-PSMF1 antibody (ab140497)

Predicted band size: 30 kDa

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Western blot - Anti-PSMF1 antibody (AB140497)

All lanes:

Western blot - Anti-PSMF1 antibody (ab140497) at 0.4 µg/mL

Lane 1:

293T whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Predicted band size: 30 kDa

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Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human Proteasome inhibitor PI31 subunit aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q92530

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Bat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab140497 was affinity purified using an epitope specific to PSMF1 immobilized on a solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PSMF1 also known as proteasome inhibitor subunit 1 or PI31 inhibits the proteasome a protein complex responsible for degrading unwanted or damaged proteins through ubiquitin-proteasome pathway. PSMF1 has a mass of approximately 29 kDa and shows expression in a variety of human tissues with notable presence in the liver brain and testis. Its role as an inhibitor allows regulation of proteasomal activity playing important part in maintaining protein homeostasis.

Biological function summary

PSMF1 interacts with proteasomal subunits functioning within the core particle of the 20S proteasome complex. Its inhibitory action helps balance protein degradation in cells impacting processes such as cell cycle apoptosis and stress responses. The modulating effect of PSMF1 on the proteasome distinguishes its role in cellular regulatory mechanisms.

Pathways

PSMF1 contributes to the ubiquitin-proteasome pathway and influences the NF-kB signaling pathway. PSMF1's interactions within this system connect it with other essential proteins including ubiquitin which mark proteins for degradation and the proteasome the complex where degradation occurs. This process affects the transcription factor NF-kB which regulates genes involved in immune response and inflammation.

PSMF1 has been associated with cancer and neurodegenerative diseases like Parkinson’s disease. In cancer its role in regulating proteasome activity impacts cellular proliferation and survival connecting it to other proteins such as p53 involved in tumor suppression. In neurodegenerative disorders impaired proteasome function links PSMF1 to the accumulation of misfolded proteins with proteins like alpha-synuclein involved in the pathology of conditions such as Parkinson’s disease.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays an important role in control of proteasome function. Inhibits the hydrolysis of protein and peptide substrates by the 20S proteasome. Also inhibits the activation of the proteasome by the proteasome regulatory proteins PA700 and PA28.

See full target information Proteasome inhibitor PI31 subunit

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 12:3928-3945 PubMed35664068

2022

5-HT2B-mediated serotonin activation in enterocytes suppresses colitis-associated cancer initiation and promotes cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Liyuan Mao,Fang Xin,Jie Ren,Shuai Xu,Haixia Huang,Xu Zha,Xinxin Wen,Guoqing Gu,Guang Yang,Yuan Cheng,Chen Zhang,Wei Wang,Xicheng Liu

Developmental cell 50:509-524.e10 PubMed31327739

2019

PI31 Is an Adaptor Protein for Proteasome Transport in Axons and Required for Synaptic Development.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Liu,Sandra Jones,Adi Minis,Jose Rodriguez,Henrik Molina,Hermann Steller

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PSMF1 antibody

Immunoprecipitation - Anti-PSMF1 antibody (AB140497)

Western blot - Anti-PSMF1 antibody (AB140497)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise