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AB187200

Anti-PSMF1 antibody

1

(1 Review)

|

(1 Publication)

Rabbit Polyclonal PSMF1 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Proteasome inhibitor PI31 subunit.

View Alternative Names

Proteasome inhibitor PI31 subunit, hPI31, PSMF1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMF1 antibody (AB187200)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMF1 antibody (AB187200)

ab18720 Immunohistochemistry of paraffin-embedded human liver injury using PSMF1 antibody at dilution of 1 : 100 (x40 lens).

Western blot - Anti-PSMF1 antibody (AB187200)
  • WB

Supplier Data

Western blot - Anti-PSMF1 antibody (AB187200)

All lanes:

Western blot - Anti-PSMF1 antibody (ab187200) at 1/500 dilution

Lane 1:

SW480 cell extract

Lane 2:

A549 cell extract

Lane 3:

K562 cell extract

Lane 4:

Mouse liver tissue extract

Lane 5:

Mouse testis tissue extract

Predicted band size: 30 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human Proteasome inhibitor PI31 subunit.

Q92530

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PSMF1 also known as proteasome inhibitor subunit 1 or PI31 inhibits the proteasome a protein complex responsible for degrading unwanted or damaged proteins through ubiquitin-proteasome pathway. PSMF1 has a mass of approximately 29 kDa and shows expression in a variety of human tissues with notable presence in the liver brain and testis. Its role as an inhibitor allows regulation of proteasomal activity playing important part in maintaining protein homeostasis.
Biological function summary

PSMF1 interacts with proteasomal subunits functioning within the core particle of the 20S proteasome complex. Its inhibitory action helps balance protein degradation in cells impacting processes such as cell cycle apoptosis and stress responses. The modulating effect of PSMF1 on the proteasome distinguishes its role in cellular regulatory mechanisms.

Pathways

PSMF1 contributes to the ubiquitin-proteasome pathway and influences the NF-kB signaling pathway. PSMF1's interactions within this system connect it with other essential proteins including ubiquitin which mark proteins for degradation and the proteasome the complex where degradation occurs. This process affects the transcription factor NF-kB which regulates genes involved in immune response and inflammation.

PSMF1 has been associated with cancer and neurodegenerative diseases like Parkinson’s disease. In cancer its role in regulating proteasome activity impacts cellular proliferation and survival connecting it to other proteins such as p53 involved in tumor suppression. In neurodegenerative disorders impaired proteasome function links PSMF1 to the accumulation of misfolded proteins with proteins like alpha-synuclein involved in the pathology of conditions such as Parkinson’s disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays an important role in control of proteasome function. Inhibits the hydrolysis of protein and peptide substrates by the 20S proteasome. Also inhibits the activation of the proteasome by the proteasome regulatory proteins PA700 and PA28.
See full target information Proteasome inhibitor PI31 subunit
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 10:958 PubMed31114588

2019

NOD2 and TLR2 Signal via TBK1 and PI31 to Direct Cross-Presentation and CD8 T Cell Responses.

Applications

Unspecified application

Species

Unspecified reactive species

Daniele Corridoni,Seiji Shiraishi,Thomas Chapman,Tessa Steevels,Daniele Muraro,Marie-Laëtitia Thézénas,Gennaro Prota,Ji-Li Chen,Uzi Gileadi,Nicola Ternette,Vincenzo Cerundolo,Alison Simmons
View all publications
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Product promise

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