AB167396

Anti-PSMG1 antibody [EPR10223]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal PSMG1 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

C21LRP, DSCR2, PAC1, PSMG1, Proteasome assembly chaperone 1, PAC-1, Chromosome 21 leucine-rich protein, Down syndrome critical region protein 2, Proteasome chaperone homolog 1, C21-LRP, Pba1

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMG1 antibody [EPR10223] (AB167396)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMG1 antibody [EPR10223] (AB167396)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeing PSMG1 with ab167396 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMG1 antibody [EPR10223] (AB167396)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling PSMG1 with ab167396 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-PSMG1 antibody [EPR10223] (AB167396)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PSMG1 antibody [EPR10223] (AB167396)

Immunofluorescent analysis of HepG2 cells, labeling PSMG1 with ab167396 at 1/250 dilution.

Unknown

Immunoprecipitation - Anti-PSMG1 antibody [EPR10223] (AB167396)

Detection of PSMG1 by Western Blot of Immunprecipitate. HepG2 cell lysate immunoprecipitated using ab167396 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-PSMG1 antibody [EPR10223] (ab167396) at 1/10000 dilution

All lanes:

Immunoprecipitation pellet from HepG2 cell lysate at 10 µg

Predicted band size: 33 kDa

false

Unknown

Western blot - Anti-PSMG1 antibody [EPR10223] (AB167396)

All lanes:

Western blot - Anti-PSMG1 antibody [EPR10223] (ab167396) at 1/10000 dilution

Lane 1:

U-87 MG cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 33 kDa

false

Carrier free

Anti-PSMG1 antibody [EPR10223] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10223

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PSMG1 also known as Proteasome Assembly Chaperone 1 or PAC1 is involved in the assembly of the 20S proteasome complex which is essential for protein degradation. This protein has a mass of approximately 36 kDa. Researchers have identified its expression primarily in the cytoplasm and nucleus of various cell types across different human tissues suggesting a broad functional role.

Biological function summary

PSMG1 participates as a chaperone that guides the proper assembly of the proteasome complex. It partners with other chaperones such as PSMG2 to help ensure that the proteasome particles achieve their correct configuration. By doing so it maintains protein homeostasis within cells which is essential for cellular function and survival. Its activity is critical for regulating the protein quality control system.

Pathways

PSMG1 plays a role in the ubiquitin-proteasome pathway which is responsible for protein degradation and turnover. This protein also interacts with other components of the pathway such as ubiquitin and various proteasome subunits. PSMG1's function within these pathways highlights its interaction with proteins involved in regulating the cell cycle and apoptosis ensuring cells respond properly to stress and damage.

PSMG1 has been linked to conditions such as cancer and neurodegenerative diseases. Abnormalities in its function can lead to disruptions in the proteasome pathway affecting proteins like p53 an important regulator of cell cycle and apoptosis. These links point to the importance of PSMG1 in maintaining cellular integrity and underline its significance in disease progression offering potential insights for therapeutic targets.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Chaperone protein which promotes assembly of the 20S proteasome as part of a heterodimer with PSMG2. The PSMG1-PSMG2 heterodimer binds to the PSMA5 and PSMA7 proteasome subunits, promotes assembly of the proteasome alpha subunits into the heteroheptameric alpha ring and prevents alpha ring dimerization.

See full target information PSMG1

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 7:9365 PubMed28839214

2017

The role of the immunoproteasome in interferon-γ-mediated microglial activation.

Applications

Unspecified application

Species

Unspecified reactive species

Kasey E Moritz,Nikki M McCormack,Mahlet B Abera,Coralie Viollet,Young J Yauger,Gauthaman Sukumar,Clifton L Dalgard,Barrington G Burnett

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PSMG1 antibody [EPR10223]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMG1 antibody [EPR10223] (AB167396)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMG1 antibody [EPR10223] (AB167396)

Immunocytochemistry/ Immunofluorescence - Anti-PSMG1 antibody [EPR10223] (AB167396)

Immunoprecipitation - Anti-PSMG1 antibody [EPR10223] (AB167396)

Western blot - Anti-PSMG1 antibody [EPR10223] (AB167396)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise