Anti-Psoriasin antibody [EPR23482-38]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Psoriasin antibody [EPR23482-38] (AB275026)

Immunohistochemical analysis of paraffin-embedded human skin tissue labeling Psoriasin with ab275026 at 1/500 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human skin (PMID : 18223693). The section was incubated with ab275026 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Psoriasin antibody [EPR23482-38] (AB275026)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MDA-MB-468 (Human breast adenocarcinoma epithelial cell) cells labelling Psoriasin with ab275026 at 1/500 dilution (0.1μg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Psoriasin antibody [EPR23482-38] (AB275026)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Psoriasin with ab275026 at 1/500 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human tonsil. The section was incubated with ab275026 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Psoriasin antibody [EPR23482-38] (AB275026)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MDA-MB-468 cells labelling Psoriasin with ab275026 at 1/250 (2.132 μg/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in MDA-MB-468 cells.

Negative control : MDA-MB-231 (PMID : 15994944).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.

• IP

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Immunoprecipitation - Anti-Psoriasin antibody [EPR23482-38] (AB275026)

Psoriasin was immunoprecipitated from 0.35 mg MDA-MB-468 (human breast adenocarcinoma epithelial cell), whole cell lysate with ab275026 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275026 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : MDA-MB-468 whole cell lysate 10 μg.

Lane 2 : ab275026 IP in MDA-MB-468 whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab275026 in MDA-MB-468 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 76 seconds.

All lanes:

Immunoprecipitation - Anti-Psoriasin antibody [EPR23482-38] (ab275026)

Predicted band size: 11 kDa

Observed band size: 12 kDa

false

• WB

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Western blot - Anti-Psoriasin antibody [EPR23482-38] (AB275026)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 81 seconds.

The expression profile observed is consistent with what has been described in the literature (PMID : 20596736, 12855640, 26055798).

All lanes:

Western blot - Anti-Psoriasin antibody [EPR23482-38] (ab275026) at 1/1000 dilution

All lanes:

Human tonsil tissue lysate at 20 µg

Secondary

All lanes:

VeriBlot for IP secondary antibody(HRP)(<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 11 kDa

Observed band size: 12 kDa

false

• WB

Supplier Data

Western blot - Anti-Psoriasin antibody [EPR23482-38] (AB275026)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

The expression profile observed is consistent with what has been described in the literature (PMID : 20596736, 12855640, 26055798).

Negative control : MDA-MB-231 (PMID : 12855640).

All lanes:

Western blot - Anti-Psoriasin antibody [EPR23482-38] (ab275026) at 1/1000 dilution

Lane 1:

MDA-MB-468 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 11 kDa

Observed band size: 12 kDa

false

• WB

Unknown

Western blot - Anti-Psoriasin antibody [EPR23482-38] (AB275026)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 8 seconds.

This antibody reacts with Psoriasin (Protein S100-A7) and Protein S100-A7A.
Linking with 6x His tag leads to the shift of the bands .
Both recombinant proteins were made in house and extracted from E.coli expressing Psoriasin (Protein S100-A7) or Protein S100-A7A.

All lanes:

Western blot - Anti-Psoriasin antibody [EPR23482-38] (ab275026) at 1/1000 dilution

Lane 1:

His-tagged human recombinant Psoriasin (Protein S100-A7) protein, 500 ng

Lane 2:

His-tagged human recombinant S100-A7A protein, 500 ng

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 11 kDa

Observed band size: 16 kDa

false