Rabbit Recombinant Monoclonal Psoriasin antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra), IHC-P, IP and reacts with Human, Recombinant fragment - Human samples.
Images
![Western blot - Anti-Psoriasin antibody [EPR23482-38] - BSA and Azide free (AB275040), expandable thumbnail](https://content.abcam.com/products/images/psoriasin-antibody-epr23482-38-bsa-and-azide-free-ab275040--western-blot-img835.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Psoriasin antibody [EPR23482-38] - BSA and Azide free (AB275040)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Psoriasin antibody [EPR23482-38] - BSA and Azide free (AB275040), expandable thumbnail](https://content.abcam.com/products/images/psoriasin-antibody-epr23482-38-bsa-and-azide-free-ab275040--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img41120.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Psoriasin antibody [EPR23482-38] - BSA and Azide free (AB275040)")
![Immunoprecipitation - Anti-Psoriasin antibody [EPR23482-38] - BSA and Azide free (AB275040), expandable thumbnail](https://content.abcam.com/products/images/psoriasin-antibody-epr23482-38-bsa-and-azide-free-ab275040--immunoprecipitation-img7780.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Psoriasin antibody [EPR23482-38] - BSA and Azide free (AB275040)")
![Immunocytochemistry/ Immunofluorescence - Anti-Psoriasin antibody [EPR23482-38] - BSA and Azide free (AB275040), expandable thumbnail](https://content.abcam.com/products/images/psoriasin-antibody-epr23482-38-bsa-and-azide-free-ab275040--immunocytochemistry-immunofluorescence-img128781.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Psoriasin antibody [EPR23482-38] - BSA and Azide free (AB275040)")
![Flow Cytometry (Intracellular) - Anti-Psoriasin antibody [EPR23482-38] - BSA and Azide free (AB275040), expandable thumbnail](https://content.abcam.com/products/images/psoriasin-antibody-epr23482-38-bsa-and-azide-free-ab275040--flow-cytometry-intracellular-img80100.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Psoriasin antibody [EPR23482-38] - BSA and Azide free (AB275040)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | WB | Flow Cyt (Intra) | IHC-P | IP | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Recombinant fragment - Human | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human, Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Recombinant fragment - Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Alternative names
PSOR1, S100A7C, S100A7, Protein S100-A7, Psoriasin, S100 calcium-binding protein A7
Recommended products
Rabbit Recombinant Monoclonal Psoriasin antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra), IHC-P, IP and reacts with Human, Recombinant fragment - Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR23482-38
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab275040 is the carrier-free version of Anti-Psoriasin antibody [EPR23482-38] ab275026.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Psoriasin also known as S100A7 is a member of the S100 protein family. It has a molecular mass of approximately 11.4 kDa. This protein exhibits calcium binding properties that influence its function. Psoriasin primarily expresses in keratinocytes which are predominant in the epidermis of the skin. Additionally it appears in various epithelia like mucous membranes. The expression levels rise in certain skin conditions suggesting it has a role in skin physiology.
- Biological function summary
Psoriasin acts as an antimicrobial peptide contributing to the skin’s defense system against microbial invasion. It helps in controlling the growth of bacteria like E. coli and fungi. The protein does not function as part of a larger complex. Instead it operates independently to maintain the integrity of the skin barrier. Psoriasin also shows involvement in the inflammatory response of the skin where it interact with other immune system players.
- Pathways
Several biological systems involve psoriasin including inflammation and skin homeostasis pathways. It interacts closely with other S100 proteins such as S100A8 and S100A9 which are part of the inflammatory process. These interactions can regulate immune cell migration and cytokine production highlighting its active role in modulating skin immune responses. Psoriasin serves as an important component in maintaining the balance between immune protection and inflammation within these pathways.
- Associated diseases and disorders
Psoriasin associates with psoriasis and breast cancer. In psoriasis it significantly increases aligning with its role in inflammation and skin barrier maintenance. The upregulation of psoriasin in psoriasis has been linked to overexpression of related proteins like S100A8 contributing to the chronic inflammatory state. In breast cancer particularly in certain carcinoma contexts its presence suggests a role in cancer cell survival. Researchers study its interaction with proteins in the cancer microenvironment to better understand its influence on tumor progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Western blot - Anti-Psoriasin antibody [EPR23482-38] - BSA and Azide free (ab275040), expandable thumbnail](https://content.abcam.com/products/images/psoriasin-antibody-epr23482-38-bsa-and-azide-free-ab275040--western-blot-img835.jpg "Western blot - Anti-Psoriasin antibody [EPR23482-38] - BSA and Azide free (ab275040)")
Western blot - Anti-Psoriasin antibody [EPR23482-38] - BSA and Azide free (ab275040)
This data was developed using Anti-Psoriasin antibody [EPR23482-38] ab275026, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 81 seconds.
The expression profile observed is consistent with what has been described in the literature (PMID: 20596736, 12855640, 26055798).
All lanes: Western blot - Anti-Psoriasin antibody [EPR23482-38] (Anti-Psoriasin antibody [EPR23482-38] ab275026) at 1/1000 dilution
All lanes: Human tonsil tissue lysate at 20 µg
SecondaryAll lanes: VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Predicted band size: 11 kDa
Observed band size: 12 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Psoriasin antibody [EPR23482-38] - BSA and Azide free (ab275040), expandable thumbnail](https://content.abcam.com/products/images/psoriasin-antibody-epr23482-38-bsa-and-azide-free-ab275040--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img41120.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Psoriasin antibody [EPR23482-38] - BSA and Azide free (ab275040)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Psoriasin antibody [EPR23482-38] - BSA and Azide free (ab275040)
This data was developed using Anti-Psoriasin antibody [EPR23482-38] ab275026, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human skin tissue labeling Psoriasin with Anti-Psoriasin antibody [EPR23482-38] ab275026 at 1/500 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human skin (PMID: 18223693). The section was incubated with Anti-Psoriasin antibody [EPR23482-38] ab275026 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunoprecipitation - Anti-Psoriasin antibody [EPR23482-38] - BSA and Azide free (ab275040), expandable thumbnail](https://content.abcam.com/products/images/psoriasin-antibody-epr23482-38-bsa-and-azide-free-ab275040--immunoprecipitation-img7780.jpg "Immunoprecipitation - Anti-Psoriasin antibody [EPR23482-38] - BSA and Azide free (ab275040)")
Immunoprecipitation - Anti-Psoriasin antibody [EPR23482-38] - BSA and Azide free (ab275040)
This data was developed using Anti-Psoriasin antibody [EPR23482-38] ab275026, the same antibody clone in a different buffer formulation.
Psoriasin was immunoprecipitated from 0.35 mg MDA-MB-468 (human breast adenocarcinoma epithelial cell), whole cell lysate with Anti-Psoriasin antibody [EPR23482-38] ab275026 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Psoriasin antibody [EPR23482-38] ab275026 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: MDA-MB-468 whole cell lysate 10 μg.
Lane 2: Anti-Psoriasin antibody [EPR23482-38] ab275026 IP in MDA-MB-468 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Psoriasin antibody [EPR23482-38] ab275026 in MDA-MB-468 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 76 seconds.
All lanes: Immunoprecipitation - Anti-Psoriasin antibody [EPR23482-38] (Anti-Psoriasin antibody [EPR23482-38] ab275026)
Predicted band size: 11 kDa
Observed band size: 12 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Psoriasin antibody [EPR23482-38] - BSA and Azide free (ab275040), expandable thumbnail](https://content.abcam.com/products/images/psoriasin-antibody-epr23482-38-bsa-and-azide-free-ab275040--immunocytochemistry-immunofluorescence-img128781.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Psoriasin antibody [EPR23482-38] - BSA and Azide free (ab275040)")
Immunocytochemistry/ Immunofluorescence - Anti-Psoriasin antibody [EPR23482-38] - BSA and Azide free (ab275040)
This data was developed using Anti-Psoriasin antibody [EPR23482-38] ab275026, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MDA-MB-468 cells labelling Psoriasin with Anti-Psoriasin antibody [EPR23482-38] ab275026 at 1/250 (2.132 μg/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in MDA-MB-468 cells.
Negative control: MDA-MB-231 (PMID: 15994944).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
![Flow Cytometry (Intracellular) - Anti-Psoriasin antibody [EPR23482-38] - BSA and Azide free (ab275040), expandable thumbnail](https://content.abcam.com/products/images/psoriasin-antibody-epr23482-38-bsa-and-azide-free-ab275040--flow-cytometry-intracellular-img80100.jpg "Flow Cytometry (Intracellular) - Anti-Psoriasin antibody [EPR23482-38] - BSA and Azide free (ab275040)")
Flow Cytometry (Intracellular) - Anti-Psoriasin antibody [EPR23482-38] - BSA and Azide free (ab275040)
This data was developed using Anti-Psoriasin antibody [EPR23482-38] ab275026, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MDA-MB-468 (Human breast adenocarcinoma epithelial cell) cells labelling Psoriasin with Anti-Psoriasin antibody [EPR23482-38] ab275026 at 1/500 dilution (0.1μg) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
A Goat anti rabbit IgG (Alexa Fluor®488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
![Western blot - Anti-Psoriasin antibody [EPR23482-38] - BSA and Azide free (ab275040), expandable thumbnail](https://content.abcam.com/products/images/psoriasin-antibody-epr23482-38-bsa-and-azide-free-ab275040--western-blot-img246.jpg "Western blot - Anti-Psoriasin antibody [EPR23482-38] - BSA and Azide free (ab275040)")
Western blot - Anti-Psoriasin antibody [EPR23482-38] - BSA and Azide free (ab275040)
This data was developed using Anti-Psoriasin antibody [EPR23482-38] ab275026, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 8 seconds.
This antibody reacts with Psoriasin (Protein S100-A7) and Protein S100-A7A.
Linking with 6x His tag leads to the shift of the bands .
Both recombinant proteins were made in house and extracted from E.coli expressing Psoriasin (Protein S100-A7) or Protein S100-A7A.All lanes: Western blot - Anti-Psoriasin antibody [EPR23482-38] (Anti-Psoriasin antibody [EPR23482-38] ab275026) at 1/1000 dilution
Lane 1: His-tagged human recombinant Psoriasin (Protein S100-A7) protein, 500 ng
Lane 2: His-tagged human recombinant S100-A7A protein, 500 ng
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 11 kDa
Observed band size: 16 kDa
![Western blot - Anti-Psoriasin antibody [EPR23482-38] - BSA and Azide free (ab275040), expandable thumbnail](https://content.abcam.com/products/images/psoriasin-antibody-epr23482-38-bsa-and-azide-free-ab275040--western-blot-img3806.jpg "Western blot - Anti-Psoriasin antibody [EPR23482-38] - BSA and Azide free (ab275040)")
Western blot - Anti-Psoriasin antibody [EPR23482-38] - BSA and Azide free (ab275040)
This data was developed using Anti-Psoriasin antibody [EPR23482-38] ab275026, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer:5% NFDM/TBST.
Exposure time: 3 minutes.
The expression profile observed is consistent with what has been described in the literature (PMID: 20596736, 12855640, 26055798).
Negative control: MDA-MB-231 (PMID: 12855640).
All lanes: Western blot - Anti-Psoriasin antibody [EPR23482-38] (Anti-Psoriasin antibody [EPR23482-38] ab275026) at 1/1000 dilution
Lane 1: MDA-MB-468 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 2: MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 11 kDa
Observed band size: 12 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Psoriasin antibody [EPR23482-38] - BSA and Azide free (ab275040), expandable thumbnail](https://content.abcam.com/products/images/psoriasin-antibody-epr23482-38-bsa-and-azide-free-ab275040--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img64369.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Psoriasin antibody [EPR23482-38] - BSA and Azide free (ab275040)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Psoriasin antibody [EPR23482-38] - BSA and Azide free (ab275040)
This data was developed using Anti-Psoriasin antibody [EPR23482-38] ab275026, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Psoriasin with Anti-Psoriasin antibody [EPR23482-38] ab275026 at 1/500 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human tonsil. The section was incubated with Anti-Psoriasin antibody [EPR23482-38] ab275026 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Western blot - Anti-Psoriasin antibody [EPR23482-38] - BSA and Azide free (ab275040), expandable thumbnail](https://content.abcam.com/products/images/psoriasin-antibody-epr23482-38-bsa-and-azide-free-ab275040--western-blot-img246.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Psoriasin antibody [EPR23482-38] - BSA and Azide free (ab275040)")
![Western blot - Anti-Psoriasin antibody [EPR23482-38] - BSA and Azide free (ab275040), expandable thumbnail](https://content.abcam.com/products/images/psoriasin-antibody-epr23482-38-bsa-and-azide-free-ab275040--western-blot-img3806.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Psoriasin antibody [EPR23482-38] - BSA and Azide free (ab275040)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Psoriasin antibody [EPR23482-38] - BSA and Azide free (ab275040), expandable thumbnail](https://content.abcam.com/products/images/psoriasin-antibody-epr23482-38-bsa-and-azide-free-ab275040--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img64369.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Psoriasin antibody [EPR23482-38] - BSA and Azide free (ab275040)")
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