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AB214012

Anti-PSPC1 antibody [1L4] - C-terminal

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(1 Publication)

Mouse Monoclonal PSPC1 antibody. C-terminal. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Mouse Pspc1 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

PSP1, PSPC1, Paraspeckle component 1, Paraspeckle protein 1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-PSPC1 antibody [1L4] - C-terminal (AB214012)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PSPC1 antibody [1L4] - C-terminal (AB214012)

Immunofluorescent analysis of HeLa cells,4% paraformaldehyde fixed and 0.5% Triton X-100 permeabilized, labeling PSPC1 with ab214012 at 5 μg/mL dilution. Secondary antibody : Goat Anti-Mouse IgG, Cy3 conjugate.

Western blot - Anti-PSPC1 antibody [1L4] - C-terminal (AB214012)
  • WB

Supplier Data

Western blot - Anti-PSPC1 antibody [1L4] - C-terminal (AB214012)

Lane 1:

Western blot - Anti-PSPC1 antibody [1L4] - C-terminal (ab214012) at 0.5 µg/mL

Lane 2:

Western blot - Anti-PSPC1 antibody [1L4] - C-terminal (ab214012) at 1 µg/mL

Lane 3:

Western blot - Anti-PSPC1 antibody [1L4] - C-terminal (ab214012) at 2 µg/mL

All lanes:

Hepa1-6 whole cell lysate

Secondary

All lanes:

Goat Anti-Mouse IgG-Peroxidase

Predicted band size: 59 kDa

true

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1L4

Isotype

IgG1

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Mouse Pspc1 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q8R326

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5-10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purity
IgG fraction
Purification notes
Purified Immunoglobulin
Storage buffer
pH: 7.4 Preservative: 0.0975% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PSPC1 also known as Paraspeckle Component 1 is a nuclear RNA-binding protein with a mass of approximately 53 kDa. This protein plays an important mechanical role in the formation of paraspeckles subnuclear structures involved in RNA processing and regulation. PSPC1 predominantly localizes in the nucleus and associates with non-coding RNAs such as NEAT1 contributing to nuclear body dynamics. Though present in several tissues its expression is notably high in testis and embryonic stem cells reflecting its roles in cellular processes.
Biological function summary

PSPC1 participates in the regulation of gene expression by modulating the transcriptional activity and stability of specific RNAs. It functions as part of a larger RNA-protein complex that involves core paraspeckle proteins such as NONO and SFPQ. This complex helps in retaining hyper-edited RNAs within the nucleus preventing them from undergoing translation or degradation. By doing so PSPC1 influences cellular stress responses and development among other biological processes.

Pathways

PSPC1 integrates into the cellular landscape by impacting the RNA processing and nuclear organization pathways. It maintains RNA homeostasis and transcript diversity acting synergistically with related proteins like NONO and SFPQ. Within the context of these pathways paraspeckle-associated activities involve RNA editing and sequestration linking PSPC1 to critical cellular functions such as transcriptional regulation and response to environmental changes.

PSPC1's association with various conditions occurs through its regulatory mechanisms in gene expression. Aberrations in PSPC1 expression or function may contribute to cancer progression particularly in prostate cancer by influencing oncogenic signaling pathways. Moreover its interactions with other proteins such as NONO can impact neurodegenerative disorders through altered RNA metabolism and neuronal gene expression. Understanding PSPC1's roles aids in developing targeted therapies for these conditions by modulating its function or associated pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

RNA-binding protein required for the formation of nuclear paraspeckles (PubMed : 22416126). Binds to poly(A), poly(G) and poly(U) RNA homopolymers (PubMed : 22416126). Regulates, cooperatively with NONO and SFPQ, androgen receptor-mediated gene transcription activity in Sertoli cell line (By similarity). Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer (By similarity). Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway (PubMed : 28712728).
See full target information PSPC1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine : PubMed33169942

2020

Metformin attenuates trauma-induced heterotopic ossification via inhibition of Bone Morphogenetic Protein signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Lin,Fuli Shi,Shanshan Jiang,Yuanyuan Wang,Junrong Zou,Ying Ying,Deqiang Huang,Lingyu Luo,Xiaohua Yan,Zhijun Luo
View all publications
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Product promise

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