AB104238

Anti-PSPC1 antibody

Name: Anti-PSPC1 antibody (ab104238) | Abcam
Brand: Abcam Limited
SKU: AB104238
Price: 470 USD
Availability: InStock

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(6 Publications)

Rabbit Polyclonal PSPC1 antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 6 publications.

View Alternative Names

PSP1, PSPC1, Paraspeckle component 1, Paraspeckle protein 1

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-PSPC1 antibody (AB104238)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-PSPC1 antibody (AB104238)

ab104238 staining PSPC1 in HepG2 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab104238 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

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Western blot - Anti-PSPC1 antibody (AB104238)

All lanes:

Western blot - Anti-PSPC1 antibody (ab104238) at 1 µg/mL

Lane 1:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg

Lane 2:

U2OS (Human osteosarcoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 59 kDa

Observed band size: 62 kDa

true

Exposure time: 2min

Lab

Immunoprecipitation - Anti-PSPC1 antibody (AB104238)

PSPC1 was immunoprecipitated using 0.5mg Hek293 whole cell extract, 5μg of Rabbit polyclonal to and 50μl of protein G magnetic beads (+). No antibody was added to the control (-).

The antibody was incubated under agitation with Protein G beads for 10min, Hek293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

Proteins were eluted by addition of 40μl SDS loading buffer and incubated for 10min at 70°C; 10μl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab104238.

Secondary : Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

Band : 62kDa, non specific bands - 50 and 55kDa : We are unsure as to the identity of this extra band; PSPC1

All lanes:

Immunoprecipitation - Anti-PSPC1 antibody (ab104238)

Predicted band size: 59 kDa

true

Exposure time: 4min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "5 µg/mL", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chinese hamster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Macaque monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 98.98% PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PSPC1 also known as Paraspeckle Component 1 is a nuclear RNA-binding protein with a mass of approximately 53 kDa. This protein plays an important mechanical role in the formation of paraspeckles subnuclear structures involved in RNA processing and regulation. PSPC1 predominantly localizes in the nucleus and associates with non-coding RNAs such as NEAT1 contributing to nuclear body dynamics. Though present in several tissues its expression is notably high in testis and embryonic stem cells reflecting its roles in cellular processes.

Biological function summary

PSPC1 participates in the regulation of gene expression by modulating the transcriptional activity and stability of specific RNAs. It functions as part of a larger RNA-protein complex that involves core paraspeckle proteins such as NONO and SFPQ. This complex helps in retaining hyper-edited RNAs within the nucleus preventing them from undergoing translation or degradation. By doing so PSPC1 influences cellular stress responses and development among other biological processes.

Pathways

PSPC1 integrates into the cellular landscape by impacting the RNA processing and nuclear organization pathways. It maintains RNA homeostasis and transcript diversity acting synergistically with related proteins like NONO and SFPQ. Within the context of these pathways paraspeckle-associated activities involve RNA editing and sequestration linking PSPC1 to critical cellular functions such as transcriptional regulation and response to environmental changes.

PSPC1's association with various conditions occurs through its regulatory mechanisms in gene expression. Aberrations in PSPC1 expression or function may contribute to cancer progression particularly in prostate cancer by influencing oncogenic signaling pathways. Moreover its interactions with other proteins such as NONO can impact neurodegenerative disorders through altered RNA metabolism and neuronal gene expression. Understanding PSPC1's roles aids in developing targeted therapies for these conditions by modulating its function or associated pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

RNA-binding protein required for the formation of nuclear paraspeckles (PubMed : 22416126). Binds to poly(A), poly(G) and poly(U) RNA homopolymers (PubMed : 22416126). Regulates, cooperatively with NONO and SFPQ, androgen receptor-mediated gene transcription activity in Sertoli cell line (By similarity). Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer (By similarity). Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway (PubMed : 28712728).

See full target information PSPC1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 12:5451-5469 PubMed35910786

2022

Targeting the splicing factor NONO inhibits GBM progression through GPX1 intron retention.

Applications

Unspecified application

Species

Unspecified reactive species

Xu Wang,Mingzhi Han,Shuai Wang,Yanfei Sun,Wenbo Zhao,Zhiyi Xue,Xiangjun Liang,Bin Huang,Gang Li,Anjing Chen,Xingang Li,Jian Wang

Communications biology 4:1207 PubMed34675360

2021

Speckles and paraspeckles coordinate to regulate HSV-1 genes transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Li,Ziqiang Wang

Protein & cell 11:641-660 PubMed32458346

2020

Live cell imaging and proteomic profiling of endogenous NEAT1 lncRNA by CRISPR/Cas9-mediated knock-in.

Applications

Unspecified application

Species

Unspecified reactive species

Bohong Chen,Shengcheng Deng,Tianyu Ge,Miaoman Ye,Jianping Yu,Song Lin,Wenbin Ma,Zhou Songyang

Neoplasia (New York, N.Y.) 21:740-751 PubMed31220736

2019

The FUS-DDIT3 Interactome in Myxoid Liposarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jamie S E Yu,Shane Colborne,Christopher S Hughes,Gregg B Morin,Torsten O Nielsen

Cellular and molecular life sciences : CMLS 74:1117-1131 PubMed27783096

2016

NEAT1 modulates herpes simplex virus-1 replication by regulating viral gene transcription.

Applications

IF, WB

Species

Unspecified reactive species, Unspecified reactive species

Ziqiang Wang,Ping Fan,Yiwan Zhao,Shikuan Zhang,Jinhua Lu,Weidong Xie,Yuyang Jiang,Fan Lei,Naihan Xu,Yaou Zhang

Nucleic acids research 43:4569-78 PubMed25855809

2015

2'-Fluoro-modified phosphorothioate oligonucleotide can cause rapid degradation of P54nrb and PSF.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Shen,Xue-Hai Liang,Hong Sun,Stanley T Crooke

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-PSPC1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-PSPC1 antibody (AB104238)

Western blot - Anti-PSPC1 antibody (AB104238)

Immunoprecipitation - Anti-PSPC1 antibody (AB104238)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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