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AB273463

Anti-PspCas13b antibody [12F2-A10] - BSA and Azide free

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Mouse Recombinant Monoclonal PspCas13b antibody. Carrier free. Suitable for WB and reacts with Transfected cell lysate - Prevotella pectinovora samples.
2 Images
Western blot - Anti-PspCas13b antibody [12F2-A10] - BSA and Azide free (AB273463)
  • WB

Supplier Data

Western blot - Anti-PspCas13b antibody [12F2-A10] - BSA and Azide free (AB273463)

This image was produced using ab273440, the same clone in a different formulation.

Lysates should be made freshly and used in WB immediately to minimize protein degradation.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PspCas13b antibody [12F2-A10] (<a href='/en-us/products/primary-antibodies/pspcas13b-antibody-12f2-a10-ab273440'>ab273440</a>) at 1/5000 dilution

Lane 1:

HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate at 10 µg

Lane 2:

HEK-293T transfected with PspCas13b expression vector containing a myc-His-tag®, whole cell lysate at 10 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/20000 dilution

false

Exposure time: 10s

Western blot - Anti-PspCas13b antibody [12F2-A10] - BSA and Azide free (AB273463)
  • WB

Supplier Data

Western blot - Anti-PspCas13b antibody [12F2-A10] - BSA and Azide free (AB273463)

This image was produced using ab273440, the same clone in a different formulation.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PspCas13b antibody [12F2-A10] (<a href='/en-us/products/primary-antibodies/pspcas13b-antibody-12f2-a10-ab273440'>ab273440</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Lane 2:

HEK-293T (human embryonic kidney epithelial cell), whole cell lysate at 10 µg

Lane 3:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 10 µg

Lane 4:

PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 10 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/20000 dilution

false

Exposure time: 3min

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

12F2-A10

Isotype

IgG2a

Carrier free

Yes

Reacts with

Prevotella pectinovora

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Reacts with Prevotella sp. P5, 125

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Complementary Products

Primary Antibodies

AB273440

Anti-PspCas13b antibody [12F2-A10]

Recombinant|Trial Size

Western blot - Anti-PspCas13b antibody [12F2-A10] (AB273440)

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Western blot - Anti-PPIL3 antibody (AB251870)

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Primary Antibodies

AB138711

Anti-SRPP antibody [10]

Western blot - Anti-SRPP antibody [10] (AB138711)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Prevotella pectinovora": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate - Prevotella pectinovora": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab273463 is the carrier-free version of ab273440.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PspCas13b also known as CRISPR-associated protein 13b is an RNA-guided ribonuclease found in the CRISPR-Cas system of the bacterium Prevotella sp. P5-125. The protein consists of 1291 amino acids giving it a molecular mass of approximately 140 kDa. Researchers have discovered it primarily in bacterial species where it acts as a part of the bacterial immune system against phages. PspCas13b stands out because it exhibits programmable RNA-targeting activity making it effective at recognizing and cleaving viral RNA.
Biological function summary

PspCas13b operates by binding to CRISPR RNA (crRNA) to form an active complex. This complex targets single-stranded RNA with high specificity due to its RNA-guided endonuclease activity. Unlike many other CRISPR proteins PspCas13b does not cut DNA which prevents unwanted off-target DNA cleavage. The protein's RNA-targeting activity makes it a valuable tool for degrading specific RNA sequences in research and it functions independently without forming large multisubunit complexes.

Pathways

PspCas13b fits well into the adaptive immune responses in bacteria where it acts as part of a defense mechanism against RNA viruses and mobile genetic elements. It shows similarities with other RNA-targeting proteins like Cas9 though Cas9 targets DNA instead. Additionally PspCas13b's action is closely aligned with the ribonucleoprotein complexes where crRNA processing proteins initiate the CRISPR immune response allowing precise RNA interference.

Scientists explore PspCas13b’s potential in antiviral therapies and RNA-related diseases given its unique RNA-targeting capacity. There is evidence linking PspCas13b to therapeutic applications in viral infections by identifying and degrading viral RNA without affecting host DNA. Moreover it links to RNA-binding proteins involved in genetic disorders where RNA misregulation occurs. These features highlight the importance of PspCas13b in the development of innovative treatments targeting RNA-based illnesses.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information PspCas13b
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com