Rabbit Recombinant Monoclonal PSPH antibody. Carrier free. Suitable for ICC, IP, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Phosphoserine phosphatase aa 1 to C-terminus.
Images
![Immunocytochemistry - Anti-PSPH antibody [001] - BSA and Azide free (AB276045), expandable thumbnail](https://content.abcam.com/products/images/psph-antibody-001-bsa-and-azide-free-ab276045--immunocytochemistry-img100625.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-PSPH antibody [001] - BSA and Azide free (AB276045)")
![Immunoprecipitation - Anti-PSPH antibody [001] - BSA and Azide free (AB276045), expandable thumbnail](https://content.abcam.com/products/images/psph-antibody-001-bsa-and-azide-free-ab276045--immunoprecipitation-img23432.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-PSPH antibody [001] - BSA and Azide free (AB276045)")
![Western blot - Anti-PSPH antibody [001] - BSA and Azide free (AB276045), expandable thumbnail](https://content.abcam.com/products/images/psph-antibody-001-bsa-and-azide-free-ab276045--western-blot-img16533.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PSPH antibody [001] - BSA and Azide free (AB276045)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human Phosphoserine phosphatase aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P78330
Reactivity data
Select an application| ICC | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/100.00000 | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes 0.2-1 μL/mg of lysate |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
Target data
Function
Catalyzes the last irreversible step in the biosynthesis of L-serine from carbohydrates, the dephosphorylation of O-phospho-L-serine to L-serine (PubMed:12213811, PubMed:14673469, PubMed:15291819, PubMed:25080166, PubMed:9222972). L-serine can then be used in protein synthesis, to produce other amino acids, in nucleotide metabolism or in glutathione synthesis, or can be racemized to D-serine, a neuromodulator (PubMed:14673469). May also act on O-phospho-D-serine (Probable).
Alternative names
Phosphoserine phosphatase, PSP, PSPase, L-3-phosphoserine phosphatase, O-phosphoserine phosphohydrolase, PSPH
Recommended products
Rabbit Recombinant Monoclonal PSPH antibody. Carrier free. Suitable for ICC, IP, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Phosphoserine phosphatase aa 1 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human Phosphoserine phosphatase aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P78330
- Carrier free
- Yes
- Clone number
- 001
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Phosphoserine phosphatase (PSPH) also known as O-phosphoserine phosphohydrolase plays an essential role in serine biosynthesis by catalyzing the conversion of O-phospho-L-serine to L-serine. PSPH is a protein with a molecular weight of approximately 25 kDa. It is expressed across a wide range of tissues with notable expression in the liver kidney and brain. The enzyme activity of PSPH ensures the availability of serine a non-essential amino acid involved in multiple biochemical processes.
- Biological function summary
Phosphoserine phosphatase contributes significantly to cellular metabolism and growth by its involvement in the serine biosynthesis pathway. This pathway is important for providing serine which acts as a precursor for proteins nucleotides and other biologically important molecules. PSPH does not function as part of a larger complex; however its enzymatic action is critical for maintaining levels of serine when dietary intake is low. In addition to its role in metabolism PSPH influences cell proliferation and differentiation through providing the necessary serine reserves.
- Pathways
Phosphoserine phosphatase integrates into the serine biosynthesis pathway and shows a connection to the glycolytic pathway. The enzyme catalyzes one of the final steps in the phosphorylated serine synthesis process that branches from glycolysis. This pathway features interactions with enzymes like 3-phosphoglycerate dehydrogenase (PHGDH) and phosphoserine aminotransferase 1 (PSAT1) which work sequentially to produce 3-phosphoserine from 3-phosphoglycerate. PSPH follows these enzymes completing the conversion to serine and connects the serine biosynthesis cycle to broader metabolic activities.
- Associated diseases and disorders
Altered expression or function of phosphoserine phosphatase associates with conditions such as cancer and neurodegenerative diseases. Within oncogenesis elevated activity of PSPH can supply cancer cells with serine needed for rapid proliferation and this enzyme interplays with other metabolic proteins like PHGDH in cancer metabolism. In neurodegenerative disorders deficient PSPH activity disrupts serine availability leading to harmful effects on the nervous system. Understanding PSPH's role in these conditions can provide insights into therapeutic strategies targeting serine metabolism.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
![Immunocytochemistry - Anti-PSPH antibody [001] - BSA and Azide free (ab276045), expandable thumbnail](https://content.abcam.com/products/images/psph-antibody-001-bsa-and-azide-free-ab276045--immunocytochemistry-img100625.jpg "Immunocytochemistry - Anti-PSPH antibody [001] - BSA and Azide free (ab276045)")
Immunocytochemistry - Anti-PSPH antibody [001] - BSA and Azide free (ab276045)
Immunofluorescence staining of Human PSPH in A549 (human lung carcinoma cell line) cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with ab276045 at 1/60 dilution at 4℃ overnight. Then cells were stained with the AlexaFluor® 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue).
![Immunoprecipitation - Anti-PSPH antibody [001] - BSA and Azide free (ab276045), expandable thumbnail](https://content.abcam.com/products/images/psph-antibody-001-bsa-and-azide-free-ab276045--immunoprecipitation-img23432.jpg "Immunoprecipitation - Anti-PSPH antibody [001] - BSA and Azide free (ab276045)")
Immunoprecipitation - Anti-PSPH antibody [001] - BSA and Azide free (ab276045)
PSPH was immunoprecipitated using from:
Lane A:0.5 mg K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole Cell Lysate
Lane B:0.5 mg HepG2 (human liver hepatocellular carcinoma cell line) whole Cell Lysate
0.5 μL ab276045 and 15 μl of 50 % Protein G agarose.
Primary antibody: ab276045, at 1/1000 dilution
Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1/5000 dilution
Performed under reducing conditions.All lanes: Immunoprecipitation - Anti-PSPH antibody [001] - BSA and Azide free (ab276045)
Predicted band size: 25 kDa
![Western blot - Anti-PSPH antibody [001] - BSA and Azide free (ab276045), expandable thumbnail](https://content.abcam.com/products/images/psph-antibody-001-bsa-and-azide-free-ab276045--western-blot-img16533.jpg "Western blot - Anti-PSPH antibody [001] - BSA and Azide free (ab276045)")
Western blot - Anti-PSPH antibody [001] - BSA and Azide free (ab276045)
Developed using the Odyssey technique.
Performed under reducing conditions.All lanes: Western blot - Anti-PSPH antibody [001] - BSA and Azide free (ab276045) at 1/500 dilution
Lane 1: MCF7 whole cell lysate at 30 µg
Lane 2: K562 whole cell lysate at 30 µg
Lane 3: HepG2 whole cell lysate at 30 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution
Predicted band size: 25 kDa
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