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AB276045

Anti-PSPH antibody [001] - BSA and Azide free

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Rabbit Recombinant Monoclonal PSPH antibody. Carrier free. Suitable for ICC, IP, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Phosphoserine phosphatase aa 1 to C-terminus.

View Alternative Names

Phosphoserine phosphatase, PSP, PSPase, L-3-phosphoserine phosphatase, O-phosphoserine phosphohydrolase, PSPH

3 Images
Immunocytochemistry - Anti-PSPH antibody [001] - BSA and Azide free (AB276045)
  • ICC

Supplier Data

Immunocytochemistry - Anti-PSPH antibody [001] - BSA and Azide free (AB276045)

Immunofluorescence staining of Human PSPH in A549 (human lung carcinoma cell line) cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with ab276045 at 1/60 dilution at 4℃ overnight. Then cells were stained with the AlexaFluor® 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue).

Immunoprecipitation - Anti-PSPH antibody [001] - BSA and Azide free (AB276045)
  • IP

Unknown

Immunoprecipitation - Anti-PSPH antibody [001] - BSA and Azide free (AB276045)

PSPH was immunoprecipitated using from :
Lane A : 0.5 mg K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole Cell Lysate
Lane B : 0.5 mg HepG2 (human liver hepatocellular carcinoma cell line) whole Cell Lysate

0.5 μL ab276045 and 15 μl of 50 % Protein G agarose.
Primary antibody : ab276045, at 1/1000 dilution
Secondary antibody : Dylight 800-labeled antibody to rabbit IgG (H+L), at 1/5000 dilution

Performed under reducing conditions.

All lanes:

Immunoprecipitation - Anti-PSPH antibody [001] - BSA and Azide free (ab276045)

Predicted band size: 25 kDa

false

Western blot - Anti-PSPH antibody [001] - BSA and Azide free (AB276045)
  • WB

Unknown

Western blot - Anti-PSPH antibody [001] - BSA and Azide free (AB276045)

Developed using the Odyssey technique.

Performed under reducing conditions.

All lanes:

Western blot - Anti-PSPH antibody [001] - BSA and Azide free (ab276045) at 1/500 dilution

Lane 1:

MCF7 whole cell lysate at 30 µg

Lane 2:

K562 whole cell lysate at 30 µg

Lane 3:

HepG2 whole cell lysate at 30 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution

Predicted band size: 25 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

001

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC, WB, IP

applications

Immunogen

Recombinant Fragment Protein within Human Phosphoserine phosphatase aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P78330

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/20 - 1/100", "ICC-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>0.2-1 μL/mg of lysate</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phosphoserine phosphatase (PSPH) also known as O-phosphoserine phosphohydrolase plays an essential role in serine biosynthesis by catalyzing the conversion of O-phospho-L-serine to L-serine. PSPH is a protein with a molecular weight of approximately 25 kDa. It is expressed across a wide range of tissues with notable expression in the liver kidney and brain. The enzyme activity of PSPH ensures the availability of serine a non-essential amino acid involved in multiple biochemical processes.
Biological function summary

Phosphoserine phosphatase contributes significantly to cellular metabolism and growth by its involvement in the serine biosynthesis pathway. This pathway is important for providing serine which acts as a precursor for proteins nucleotides and other biologically important molecules. PSPH does not function as part of a larger complex; however its enzymatic action is critical for maintaining levels of serine when dietary intake is low. In addition to its role in metabolism PSPH influences cell proliferation and differentiation through providing the necessary serine reserves.

Pathways

Phosphoserine phosphatase integrates into the serine biosynthesis pathway and shows a connection to the glycolytic pathway. The enzyme catalyzes one of the final steps in the phosphorylated serine synthesis process that branches from glycolysis. This pathway features interactions with enzymes like 3-phosphoglycerate dehydrogenase (PHGDH) and phosphoserine aminotransferase 1 (PSAT1) which work sequentially to produce 3-phosphoserine from 3-phosphoglycerate. PSPH follows these enzymes completing the conversion to serine and connects the serine biosynthesis cycle to broader metabolic activities.

Altered expression or function of phosphoserine phosphatase associates with conditions such as cancer and neurodegenerative diseases. Within oncogenesis elevated activity of PSPH can supply cancer cells with serine needed for rapid proliferation and this enzyme interplays with other metabolic proteins like PHGDH in cancer metabolism. In neurodegenerative disorders deficient PSPH activity disrupts serine availability leading to harmful effects on the nervous system. Understanding PSPH's role in these conditions can provide insights into therapeutic strategies targeting serine metabolism.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the last irreversible step in the biosynthesis of L-serine from carbohydrates, the dephosphorylation of O-phospho-L-serine to L-serine (PubMed : 12213811, PubMed : 14673469, PubMed : 15291819, PubMed : 25080166, PubMed : 9222972). L-serine can then be used in protein synthesis, to produce other amino acids, in nucleotide metabolism or in glutathione synthesis, or can be racemized to D-serine, a neuromodulator (PubMed : 14673469). May also act on O-phospho-D-serine (Probable).
See full target information Phosphoserine phosphatase
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