Anti-PSPH antibody [EPR26995-88A]

6 product images

• 

  Western blot - Anti-PSPH antibody [EPR26995-88A] (ab306577)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.
  

  Low expression: MCF7 (PMID: 32783398) and A549 (PMID: 30874300).

  This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

  All lanes: Western blot - Anti-PSPH antibody [EPR26995-88A] (ab306577) at 1/1000 dilution

  Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

  Lane 2: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg

  Lane 3: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

  Lane 4: A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Developed using the ECL technique.

  Observed band size: 25 kDa

  Exposure time: 59s

• 

  Western blot - Anti-PSPH antibody [EPR26995-88A] (ab306577)

  Western blot: Anti-PSPH antibody [EPR26995-88A] (ab306577) staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab306577 was shown to bind specifically to PSPH. A band was observed at 25 kDa in wild-type A549 cell lysates with no signal observed at this size in PSPH knockout cell line. To generate this image, wild-type and PSPH knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

  All lanes: Western blot - Anti-PSPH antibody [EPR26995-88A] (ab306577) at 1/1000 dilution

  Lane 1: Wild-type A549 cell lysate at 20 µg

  Lane 2: PSPH knockout A549 cell lysate at 20 µg

  Lane 3: Caco-2 cell lysate at 20 µg

  Lane 4: A549 Nuclear cell lysate at 20 µg

  Secondary

  Lanes 1 - 4: Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution

  Lanes 1 - 4: Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

  Performed under reducing conditions.

  Observed band size: 25 kDa

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  Immunohistochemistry - Anti-PSPH antibody [EPR26995-88A] (ab306577)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Phosphoserine phosphatase with ab306577 at 1/500 (1.078 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining in human kidney. The section was incubated with ab306577 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  
  Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
  
  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• 

  Western blot - Anti-PSPH antibody [EPR26995-88A] (ab306577)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST  
  
  Exposure time: 
  
  Lane 1: 180 seconds
  
  Lane 2: 15 seconds

  All lanes: Western blot - Anti-PSPH antibody [EPR26995-88A] (ab306577) at 1/1000 dilution

  Lane 1: Human cerebellum tissue lysate 20 μg

  Lane 2: Human kidney tissue lysate 20 μg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Observed band size: 25 kDa

  Exposure time: 180s

• 

  Immunohistochemistry - Anti-PSPH antibody [EPR26995-88A] (ab306577)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling Phosphoserine phosphatase with ab306577 at 1/500 (1.078 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
  
  Negative control: no staining in human skeletal muscle.
  
  The section was incubated with ab306577 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  
  Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
  
  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• 

  Immunohistochemistry - Anti-PSPH antibody [EPR26995-88A] (ab306577)

  Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Phosphoserine phosphatase with ab306577 at 1/500 (1.078 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining in human colon. The section was incubated with ab306577 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  
  Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
  
  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.