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Rabbit Recombinant Monoclonal PSPH antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

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Images

Western blot - Anti-PSPH antibody [EPR26995-88A] - BSA and Azide free (AB306578), expandable thumbnail
  • Western blot - Anti-PSPH antibody [EPR26995-88A] - BSA and Azide free (AB306578), expandable thumbnail
  • Immunohistochemistry - Anti-PSPH antibody [EPR26995-88A] - BSA and Azide free (AB306578), expandable thumbnail
  • Western blot - Anti-PSPH antibody [EPR26995-88A] - BSA and Azide free (AB306578), expandable thumbnail
  • Immunohistochemistry - Anti-PSPH antibody [EPR26995-88A] - BSA and Azide free (AB306578), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPICC/IFIHC-PWBFlow Cyt
Human
Not recommended
Not recommended
Tested
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species

Human, Rat, Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Rat, Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Rat, Mouse

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 products for Alternative Version

    Target data

    Function

    Catalyzes the last irreversible step in the biosynthesis of L-serine from carbohydrates, the dephosphorylation of O-phospho-L-serine to L-serine (PubMed:12213811, PubMed:15291819, PubMed:9222972, PubMed:14673469, PubMed:25080166). L-serine can then be used in protein synthesis, to produce other amino acids, in nucleotide metabolism or in glutathione synthesis, or can be racemized to D-serine, a neuromodulator (PubMed:14673469). May also act on O-phospho-D-serine (Probable).

    Alternative names

    Phosphoserine phosphatase, PSP, PSPase, L-3-phosphoserine phosphatase, O-phosphoserine phosphohydrolase, PSPH

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    Rabbit Recombinant Monoclonal PSPH antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

    Alternative names

    Phosphoserine phosphatase, PSP, PSPase, L-3-phosphoserine phosphatase, O-phosphoserine phosphohydrolase, PSPH

    Key facts

    Isotype

    IgG

    Form

    Liquid

    Clonality

    Monoclonal

    Immunogen
    • The exact immunogen used to generate this antibody is proprietary information.
    Carrier free

    Yes

    Clone number

    EPR26995-88A

    Purification technique

    Affinity purification Protein A

    Concentration
    Loading...

    Storage

    Shipped at conditions

    Blue Ice

    Appropriate long-term storage conditions

    +4°C

    Storage information

    Do Not Freeze

    Notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free batch production

    For more information, read more on recombinant antibodies.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    Supplementary info

    Activity summary

    Phosphoserine phosphatase (PSPH) also known as O-phosphoserine phosphohydrolase plays an essential role in serine biosynthesis by catalyzing the conversion of O-phospho-L-serine to L-serine. PSPH is a protein with a molecular weight of approximately 25 kDa. It is expressed across a wide range of tissues with notable expression in the liver kidney and brain. The enzyme activity of PSPH ensures the availability of serine a non-essential amino acid involved in multiple biochemical processes.

    Biological function summary

    Phosphoserine phosphatase contributes significantly to cellular metabolism and growth by its involvement in the serine biosynthesis pathway. This pathway is important for providing serine which acts as a precursor for proteins nucleotides and other biologically important molecules. PSPH does not function as part of a larger complex; however its enzymatic action is critical for maintaining levels of serine when dietary intake is low. In addition to its role in metabolism PSPH influences cell proliferation and differentiation through providing the necessary serine reserves.

    Pathways

    Phosphoserine phosphatase integrates into the serine biosynthesis pathway and shows a connection to the glycolytic pathway. The enzyme catalyzes one of the final steps in the phosphorylated serine synthesis process that branches from glycolysis. This pathway features interactions with enzymes like 3-phosphoglycerate dehydrogenase (PHGDH) and phosphoserine aminotransferase 1 (PSAT1) which work sequentially to produce 3-phosphoserine from 3-phosphoglycerate. PSPH follows these enzymes completing the conversion to serine and connects the serine biosynthesis cycle to broader metabolic activities.

    Associated diseases and disorders

    Altered expression or function of phosphoserine phosphatase associates with conditions such as cancer and neurodegenerative diseases. Within oncogenesis elevated activity of PSPH can supply cancer cells with serine needed for rapid proliferation and this enzyme interplays with other metabolic proteins like PHGDH in cancer metabolism. In neurodegenerative disorders deficient PSPH activity disrupts serine availability leading to harmful effects on the nervous system. Understanding PSPH's role in these conditions can provide insights into therapeutic strategies targeting serine metabolism.

    Product promise

    We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

    In the unlikely event of one of our products not working as expected, you are covered by our product promise.

    Full details and terms and conditions can be found here:
    Terms & Conditions.

    6 product images

    • Western blot - Anti-PSPH antibody [EPR26995-88A] - BSA and Azide free (ab306578), expandable thumbnail

      Western blot - Anti-PSPH antibody [EPR26995-88A] - BSA and Azide free (ab306578)

      This data was developed using Anti-PSPH antibody [EPR26995-88A] ab306577, the same antibody clone in a different buffer formulation.

      Western blot: Anti-PSPH antibody [EPR26995-88A] (Anti-PSPH antibody [EPR26995-88A] ab306577) staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, Anti-PSPH antibody [EPR26995-88A] ab306577 was shown to bind specifically to PSPH. A band was observed at 25 kDa in wild-type A549 cell lysates with no signal observed at this size in PSPH knockout cell line. To generate this image, wild-type and PSPH knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

      All lanes: Western blot - Anti-PSPH antibody [EPR26995-88A] (Anti-PSPH antibody [EPR26995-88A] ab306577) at 1/1000 dilution

      Lane 1: Wild-type A549 cell lysate at 20 µg

      Lane 2: PSPH knockout A549 cell lysate at 20 µg

      Lane 3: Caco-2 cell lysate at 20 µg

      Lane 4: A549 Nuclear cell lysate at 20 µg

      Secondary

      Lanes 1 - 4: Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution

      Lanes 1 - 4: Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

      Performed under reducing conditions.

      Observed band size: 25 kDa

    • Western blot - Anti-PSPH antibody [EPR26995-88A] - BSA and Azide free (ab306578), expandable thumbnail

      Western blot - Anti-PSPH antibody [EPR26995-88A] - BSA and Azide free (ab306578)

      This data was developed using Anti-PSPH antibody [EPR26995-88A] ab306577, the same antibody clone in a different buffer formulation.
      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Low expression: MCF7 (PMID: 32783398) and A549 (PMID: 30874300).

      This blot was developed using a high sensitivity ECL substrate.

      Exposure time: 59 seconds.

      All lanes: Western blot - Anti-PSPH antibody [EPR26995-88A] (Anti-PSPH antibody [EPR26995-88A] ab306577) at 1/1000 dilution

      Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 20 μg

      Lane 2: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate 20 μg

      Lane 3: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 20 μg

      Lane 4: A549 (human lung carcinoma epithelial cell) whole cell lysate 20 μg

      Secondary

      All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

      Developed using the ECL technique.

      Observed band size: 25 kDa

      Exposure time: 59s

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Low expression: MCF7 (PMID: 32783398) and A549 (PMID: 30874300).

      This blot was developed using a high sensitivity ECL substrate.

      Exposure time: 59 seconds.

    • Immunohistochemistry - Anti-PSPH antibody [EPR26995-88A] - BSA and Azide free (ab306578), expandable thumbnail

      Immunohistochemistry - Anti-PSPH antibody [EPR26995-88A] - BSA and Azide free (ab306578)

      This data was developed using Anti-PSPH antibody [EPR26995-88A] ab306577, the same antibody clone in a different buffer formulation.
      Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Phosphoserine phosphatase with Anti-PSPH antibody [EPR26995-88A] ab306577 at 1/500 (1.078 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining in human kidney. The section was incubated with Anti-PSPH antibody [EPR26995-88A] ab306577 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Western blot - Anti-PSPH antibody [EPR26995-88A] - BSA and Azide free (ab306578), expandable thumbnail

      Western blot - Anti-PSPH antibody [EPR26995-88A] - BSA and Azide free (ab306578)

      This data was developed using Anti-PSPH antibody [EPR26995-88A] ab306577, the same antibody clone in a different buffer formulation.
      Blocking and diluting buffer and concentration: 5% NFDM/TBST  

      Exposure time: 

      Lane 1: 180 seconds

      Lane 2: 15 seconds

      All lanes: Western blot - Anti-PSPH antibody [EPR26995-88A] (Anti-PSPH antibody [EPR26995-88A] ab306577) at 1/1000 dilution

      Lane 1: Human cerebellum tissue lysate 20 μg

      Lane 2: Human kidney tissue lysate 20 μg

      Secondary

      All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

      Observed band size: 25 kDa

      Exposure time: 180s

      Blocking and diluting buffer and concentration: 5% NFDM/TBST  

      Exposure time: 

      Lane 1: 180 seconds

      Lane 2: 15 seconds

    • Immunohistochemistry - Anti-PSPH antibody [EPR26995-88A] - BSA and Azide free (ab306578), expandable thumbnail

      Immunohistochemistry - Anti-PSPH antibody [EPR26995-88A] - BSA and Azide free (ab306578)

      This data was developed using Anti-PSPH antibody [EPR26995-88A] ab306577, the same antibody clone in a different buffer formulation.
      Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling Phosphoserine phosphatase with Anti-PSPH antibody [EPR26995-88A] ab306577 at 1/500 (1.078 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

      Negative control: no staining in human skeletal muscle.

      The section was incubated with Anti-PSPH antibody [EPR26995-88A] ab306577 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunohistochemistry - Anti-PSPH antibody [EPR26995-88A] - BSA and Azide free (ab306578), expandable thumbnail

      Immunohistochemistry - Anti-PSPH antibody [EPR26995-88A] - BSA and Azide free (ab306578)

      This data was developed using Anti-PSPH antibody [EPR26995-88A] ab306577, the same antibody clone in a different buffer formulation.
      Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Phosphoserine phosphatase with Anti-PSPH antibody [EPR26995-88A] ab306577 at 1/500 (1.078 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining in human colon. The section was incubated with Anti-PSPH antibody [EPR26995-88A] ab306577 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

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