AB236629

Anti-PSRC2 antibody [EPR20913-32]

20ul selling size
RabMAb
Recombinant
KO Validated
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal zfc3h1 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

2 Images

Lab

Western blot - Anti-PSRC2 antibody [EPR20913-32] (AB236629)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 15 seconds

All lanes:

Western blot - Anti-PSRC2 antibody [EPR20913-32] (ab236629) at 1/1000 dilution

Lane 1:

Mouse testis tissue lysate at 20 µg

Lane 2:

Rat thymus tissue lysate at 20 µg

Lane 3:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 226 kDa

Observed band size: 260 kDa

false

Lab

Western blot - Anti-PSRC2 antibody [EPR20913-32] (AB236629)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Lysates were kindly provided by collaborator.

ab236629 Anti-PSRC2 antibody [EPR20913-32] was shown to react with PSRC2 in mouse lymph node tissue lysate in Western blot. Loss of signal was observed when PSRC2 knockout sample was used. Wild-type and PSRC2 knockout samples were subjected to SDS-PAGE. ab236629 and ab129002 (Rabbit anti-Vinculin loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/5000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100, 000 dilution for 1 hour at room temperature before imaging. he blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.

Exposure time : 15 seconds

All lanes:

Western blot - Anti-PSRC2 antibody [EPR20913-32] (ab236629) at 1/1000 dilution

Lane 1:

Mouse lymph node(WT) tissue lysate at 10 µg

Lane 2:

PSRC2 knockout mouse lymph node tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 226 kDa

Observed band size: 260 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20913-32

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PSRC2 also known as Proline/Serine-rich Coiled-coil 2 is a protein with a known mass of approximately 28 kDa. This protein is expressed in a variety of tissues with notable presence in the brain and testis. Mechanically PSRC2 participates in cellular processes through its role in cytoskeleton organization. It exhibits specific binding properties which facilitate its involvement in microtubule dynamics aiding in the stabilization or destabilization of these structures during cell division.

Biological function summary

The coordination within microtubule dynamics which PSRC2 involves itself can significantly influence cellular mitosis and overall cell cycle progression. PSRC2 may also act as part of a larger protein complex contributing to the efficient alignment and separation of chromosomes during mitotic spindle formation. This functionality highlights its pivotal role in ensuring accurate cell division and genomic stability.

Pathways

The microtubule regulatory functions of PSRC2 integrate it within the cellular cytoskeleton organization pathways. It connects closely with the mitotic spindle checkpoint pathway working alongside other proteins such as tubulins and members of the Aurora kinase family. PSRC2's role supports the fidelity of cell cycle transitions ensuring that spindle assembly and chromosome segregation proceed without interruption.

Disruptions in the function of PSRC2 can have implications for cancer progression particularly where abnormal cell division characterizes tumor growth. Aberrations in PSRC2 expression levels or its interaction network with proteins like Aurora kinases may correlate with tumorigenesis. Additionally it holds a potential link with neurodegenerative conditions given its expression in the brain where defective microtubule functions could lead to neuropathologies.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

See full target information zfc3h1

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 20:444 PubMed36224596

2022

BMSC exosome-enriched acellular fish scale scaffolds promote bone regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Yangyufan Wang,Bin Kong,Xiang Chen,Rui Liu,Yuanjin Zhao,Zhuxiao Gu,Qing Jiang

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PSRC2 antibody [EPR20913-32]

Western blot - Anti-PSRC2 antibody [EPR20913-32] (AB236629)

Western blot - Anti-PSRC2 antibody [EPR20913-32] (AB236629)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise