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AB232635

Anti-PSS-2 antibody [EPR14663] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal PSS-2 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

PSS2, PTDSS2, Phosphatidylserine synthase 2, PSS-2, PtdSer synthase 2, Serine-exchange enzyme II

2 Images
Immunoprecipitation - Anti-PSS-2 antibody [EPR14663] - BSA and Azide free (AB232635)
  • IP

Supplier Data

Immunoprecipitation - Anti-PSS-2 antibody [EPR14663] - BSA and Azide free (AB232635)

Immunoprecipitation analysis of HepG2 cells labeling PSS-2
using ab183504 at 1/50 dilution. A Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183504).

All lanes:

Immunoprecipitation - Anti-PSS-2 antibody [EPR14663] - C-terminal (<a href='/en-us/products/primary-antibodies/pss-2-antibody-epr14663-c-terminal-ab183504'>ab183504</a>)

Predicted band size: 56 kDa

Observed band size: 52 kDa

false

Immunoprecipitation - Anti-PSS-2 antibody [EPR14663] - BSA and Azide free (AB232635)
  • IP

Unknown

Immunoprecipitation - Anti-PSS-2 antibody [EPR14663] - BSA and Azide free (AB232635)

ab183504 (purified ) at 1/30 dilution (2ug) immunoprecipitating PSS-2 in HeLa whole cell lysate.

Lane 1 (input) : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10ug
Lane 2 (+) : ab183504 & HeLa whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab183504 in HeLa whole cell lysate

For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.

Blocking and diluting buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183504)

All lanes:

Immunoprecipitation - Anti-PSS-2 antibody [EPR14663] - C-terminal (<a href='/en-us/products/primary-antibodies/pss-2-antibody-epr14663-c-terminal-ab183504'>ab183504</a>)

Predicted band size: 56 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14663

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab232635 is the carrier-free version of ab183504.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PSS-2 also known as Phospholipid Scramblase 2 functions mechanically as a lipid scramblase enzyme. It mediates the bidirectional movement of phospholipids across the bilayer of cellular membranes facilitating the collapse of lipid asymmetry. PSS-2 has a molecular weight of approximately 37 kDa. It is expressed in various tissues with significant presence in the liver which indicates its importance for lipid metabolism in this organ.
Biological function summary

Phospholipid scramblase 2 contributes to lipid homeostasis and membrane dynamics. PSS-2 forms complexes with several membrane proteins playing a role in processes such as apoptosis and cell signaling. By facilitating phospholipid translocation PSS-2 helps maintain cellular membrane composition which is critical for membrane integrity and function. This activity impacts cellular processes including cell shape motility and interaction with the environment.

Pathways

The activity of phospholipid scramblase 2 integrates into lipid metabolism and apoptotic pathways. Within the lipid metabolism pathway it cooperates closely with fatty acid-binding proteins to regulate lipid distribution and metabolism. Its involvement in the apoptotic pathway partners with caspases where it contributes to phosphatidylserine externalization an important apoptosis signal. This coordination places PSS-2 as a significant player in maintaining cellular health.

Dysregulation of phospholipid scramblase 2 associates with liver disorders and cardiovascular disease. Alterations in PSS-2 expression relate to non-alcoholic fatty liver disease due to its central role in hepatic lipid metabolism. Furthermore the interaction with proteins such as annexins links PSS-2 to cardiovascular health as these interactions influence thrombosis and inflammation. Understanding PSS-2's contributions in these contexts could lead to potential therapeutic targets for managing these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes a base-exchange reaction in which the polar head group of phosphatidylethanolamine (PE) or phosphatidylcholine (PC) is replaced by L-serine (PubMed : 19014349). Catalyzes the conversion of phosphatatidylethanolamine and does not act on phosphatidylcholine (PubMed : 19014349). Can utilize both phosphatidylethanolamine (PE) plasmalogen and diacyl PE as substrate and the latter is six times better utilized, indicating the importance of an ester linkage at the sn-1 position (By similarity). Although it shows no sn-1 fatty acyl preference, exhibits significant preference towards docosahexaenoic acid (22 : 6n-3) compared with 18 : 1 or 20 : 4 at the sn-2 position (By similarity).
See full target information PTDSS2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:9664 PubMed39511206

2024

mRNA delivery enabled by metal-organic nanoparticles.

Applications

Unspecified application

Species

Unspecified reactive species

Yuang Gu,Jingqu Chen,Zhaoran Wang,Chang Liu,Tianzheng Wang,Chan-Jin Kim,Helena Durikova,Soraia Fernandes,Darryl N Johnson,Robert De Rose,Christina Cortez-Jugo,Frank Caruso
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com