AB183504

Anti-PSS-2 antibody [EPR14663] - C-terminal

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal PSS-2 antibody. C-terminal. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

PSS2, PTDSS2, Phosphatidylserine synthase 2, PSS-2, PtdSer synthase 2, Serine-exchange enzyme II

5 Images

Lab

Western blot - Anti-PSS-2 antibody [EPR14663] - C-terminal (AB183504)

All lanes:

Western blot - Anti-PSS-2 antibody [EPR14663] - C-terminal (ab183504) at 1/5000 dilution

All lanes:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 56 kDa

Observed band size: 52 kDa

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Supplier Data

Immunoprecipitation - Anti-PSS-2 antibody [EPR14663] - C-terminal (AB183504)

Immunoprecipitation analysis of HepG2 cells labeling PSS-2
using ab183504 at 1/50 dilution (lane 1). A Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody.

Rabbit monoclonal IgG (ab172730) instead of ab183504 in HepG2 whole cell lysate (Lane 2)

All lanes:

Immunoprecipitation - Anti-PSS-2 antibody [EPR14663] - C-terminal (ab183504)

Predicted band size: 56 kDa

Observed band size: 52 kDa

false

Unknown

Immunoprecipitation - Anti-PSS-2 antibody [EPR14663] - C-terminal (AB183504)

ab183504 (purified ) at 1/30 dilution (2ug) immunoprecipitating PSS-2 in HeLa whole cell lysate.
Lane 1 (input) : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10ug
Lane 2 (+) : ab183504 & HeLa whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab183504 in HeLa whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-PSS-2 antibody [EPR14663] - C-terminal (ab183504)

Predicted band size: 56 kDa

false

Supplier Data

Western blot - Anti-PSS-2 antibody [EPR14663] - C-terminal (AB183504)

All lanes:

Western blot - Anti-PSS-2 antibody [EPR14663] - C-terminal (ab183504) at 1/10000 dilution

All lanes:

Hela cell line lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

Predicted band size: 56 kDa

Observed band size: 52 kDa

false

Supplier Data

Western blot - Anti-PSS-2 antibody [EPR14663] - C-terminal (AB183504)

All lanes:

Western blot - Anti-PSS-2 antibody [EPR14663] - C-terminal (ab183504) at 1/2000 dilution

Lane 1:

HepG2 cell line lysate at 20 µg

Lane 2:

Human fetal brain tissue lysate at 20 µg

Lane 3:

Human fetal liver tissue lysate at 20 µg

Secondary

Lane 1:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

Lanes 2 - 3:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated

Predicted band size: 56 kDa

Observed band size: 52 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14663

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30 - 1/70", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PSS-2 also known as Phospholipid Scramblase 2 functions mechanically as a lipid scramblase enzyme. It mediates the bidirectional movement of phospholipids across the bilayer of cellular membranes facilitating the collapse of lipid asymmetry. PSS-2 has a molecular weight of approximately 37 kDa. It is expressed in various tissues with significant presence in the liver which indicates its importance for lipid metabolism in this organ.

Biological function summary

Phospholipid scramblase 2 contributes to lipid homeostasis and membrane dynamics. PSS-2 forms complexes with several membrane proteins playing a role in processes such as apoptosis and cell signaling. By facilitating phospholipid translocation PSS-2 helps maintain cellular membrane composition which is critical for membrane integrity and function. This activity impacts cellular processes including cell shape motility and interaction with the environment.

Pathways

The activity of phospholipid scramblase 2 integrates into lipid metabolism and apoptotic pathways. Within the lipid metabolism pathway it cooperates closely with fatty acid-binding proteins to regulate lipid distribution and metabolism. Its involvement in the apoptotic pathway partners with caspases where it contributes to phosphatidylserine externalization an important apoptosis signal. This coordination places PSS-2 as a significant player in maintaining cellular health.

Dysregulation of phospholipid scramblase 2 associates with liver disorders and cardiovascular disease. Alterations in PSS-2 expression relate to non-alcoholic fatty liver disease due to its central role in hepatic lipid metabolism. Furthermore the interaction with proteins such as annexins links PSS-2 to cardiovascular health as these interactions influence thrombosis and inflammation. Understanding PSS-2's contributions in these contexts could lead to potential therapeutic targets for managing these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes a base-exchange reaction in which the polar head group of phosphatidylethanolamine (PE) or phosphatidylcholine (PC) is replaced by L-serine (PubMed : 19014349). Catalyzes the conversion of phosphatatidylethanolamine and does not act on phosphatidylcholine (PubMed : 19014349). Can utilize both phosphatidylethanolamine (PE) plasmalogen and diacyl PE as substrate and the latter is six times better utilized, indicating the importance of an ester linkage at the sn-1 position (By similarity). Although it shows no sn-1 fatty acyl preference, exhibits significant preference towards docosahexaenoic acid (22 : 6n-3) compared with 18 : 1 or 20 : 4 at the sn-2 position (By similarity).

See full target information PTDSS2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell stem cell 24:621-636.e16 PubMed30930145

2019

The Mitochondrial Transacylase, Tafazzin, Regulates for AML Stemness by Modulating Intracellular Levels of Phospholipids.

Applications

Unspecified application

Species

Unspecified reactive species

Ayesh K Seneviratne,Mingjing Xu,Juan J Aristizabal Henao,Val A Fajardo,Zhenyue Hao,Veronique Voisin,G Wei Xu,Rose Hurren,S Kim,Neil MacLean,Xiaoming Wang,Marcela Gronda,Danny Jeyaraju,Yulia Jitkova,Troy Ketela,Michael Mullokandov,David Sharon,Geethu Thomas,Raphaël Chouinard-Watkins,James R Hawley,Caitlin Schafer,Helen Loo Yau,Zaza Khuchua,Ahmed Aman,Rima Al-Awar,Atan Gross,Steven M Claypool,Richard P Bazinet,Mathieu Lupien,Steven Chan,Daniel D De Carvalho,Mark D Minden,Gary D Bader,Ken D Stark,Paul LeBlanc,Aaron D Schimmer

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PSS-2 antibody [EPR14663] - C-terminal

Western blot - Anti-PSS-2 antibody [EPR14663] - C-terminal (AB183504)

Immunoprecipitation - Anti-PSS-2 antibody [EPR14663] - C-terminal (AB183504)

Immunoprecipitation - Anti-PSS-2 antibody [EPR14663] - C-terminal (AB183504)

Western blot - Anti-PSS-2 antibody [EPR14663] - C-terminal (AB183504)

Western blot - Anti-PSS-2 antibody [EPR14663] - C-terminal (AB183504)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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