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Anti-PTBP1 antibody [EPR9048(B)] (ab133734) is a rabbit monoclonal antibody detecting PTBP1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF. Suitable for Human, Mouse.



- KO validated for confirmed specificity

- Biophysical QC for unrivalled batch-batch consistency

- Over 20 publications

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Images

Western blot - Anti-PTBP1 antibody [EPR9048(B)] (AB133734), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTBP1 antibody [EPR9048(B)] (AB133734), expandable thumbnail
  • Western blot - Anti-PTBP1 antibody [EPR9048(B)] (AB133734), expandable thumbnail
  • Western blot - Anti-PTBP1 antibody [EPR9048(B)] (AB133734), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PTBP1 antibody [EPR9048(B)] (AB133734), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow Cyt (Intra)IP
Human
Tested
Tested
Tested
Tested
Not recommended
Mouse
Expected
Expected
Expected
Expected
Not recommended
Rat
Predicted
Predicted
Predicted
Predicted
Not recommended

Tested
Tested

Species
Human
Dilution info
1/10000 - 1/50000
Notes

-

Expected
Expected

Species
Mouse
Dilution info
1/10000 - 1/50000
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

Perform antigen retrieval before commencing with IHC staining protocol.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
1/100 - 1/250
Notes

Perform antigen retrieval before commencing with IHC staining protocol.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/250 - 1/500
Notes

-

Expected
Expected

Species
Mouse
Dilution info
1/250 - 1/500
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/2000
Notes

-

Expected
Expected

Species
Mouse
Dilution info
1/2000
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Activates exon skipping of its own pre-mRNA during muscle cell differentiation. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA. Represses the splicing of MAPT/Tau exon 10 (PubMed:15009664). Binds to polypyrimidine-rich controlling element (PCE) of CFTR and promotes exon skipping of CFTR exon 9, thereby antagonizing TIA1 and its role in exon inclusion of CFTR exon 9 (PubMed:14966131). Plays a role in the splicing of pyruvate kinase PKM by binding repressively to a polypyrimidine tract flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed:20010808). In case of infection by picornaviruses, binds to the viral internal ribosome entry site (IRES) and stimulates the IRES-mediated translation (PubMed:21518806).

Alternative names

PTB, PTBP1, Polypyrimidine tract-binding protein 1, 57 kDa RNA-binding protein PPTB-1, Heterogeneous nuclear ribonucleoprotein I, hnRNP I

Recommended products

Anti-PTBP1 antibody [EPR9048(B)] (ab133734) is a rabbit monoclonal antibody detecting PTBP1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF. Suitable for Human, Mouse.



- KO validated for confirmed specificity

- Biophysical QC for unrivalled batch-batch consistency

- Over 20 publications

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR9048(B)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-PTBP1 antibody [EPR9048(B)] (ab133734) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse samples.

What is the molecular weight of PTBP1?


Anti-PTBP1 [EPR9048(B)] (ab133734) specifically detects a band for PTBP1 (UniProt: P26599) at a molecular weight of 57kDa.

Trusted by the scientific community


Anti-PTBP1 [EPR9048(B)] (ab133734) was first used in a scientific publication in 2012 and has been cited over 20 times in peer-reviewed journals.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed


The specificity of Anti-PTBP1 antibody [EPR9048(B)] (ab133734) has been confirmed by Western blot testing in PTBP1 Knockout HAP1 cell line, Human PTBP1 knockout HeLa cell line ab265155.



Other related products


We have a range of other formats of antibody clone [EPR9048(B)] also available for your convenience:
ab133734, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-PTBP1 antibody [EPR9048(B)] ab201020, Carrier free - Anti-PTBP1 antibody [EPR9048(B)] - BSA and Azide free ab240079, PE - PE Anti-PTBP1 antibody [EPR9048(B)] ab305838, APC - APC Anti-PTBP1 antibody [EPR9048(B)] ab305839, HRP - HRP Anti-PTBP1 antibody [EPR9048(B)] ab305840, Alkaline Phosphatase - Alkaline Phosphatase Anti-PTBP1 antibody [EPR9048(B)] ab308831, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-PTBP1 antibody [EPR9048(B)] ab309768, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-PTBP1 antibody [EPR9048(B)] ab310562, Alexa Fluor® 555 - Alexa Fluor® 555 Anti-PTBP1 antibody [EPR9048(B)] ab312091, Alexa Fluor® 568 - Alexa Fluor® 568 Anti-PTBP1 antibody [EPR9048(B)] ab312571, Alexa Fluor® 750 - Alexa Fluor® 750 Anti-PTBP1 antibody [EPR9048(B)] ab321066



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PTBP1 also known as polypyrimidine tract-binding protein 1 or hnRNP I is a protein involved in RNA binding. It weighs approximately 57 kDa and is known for its expression in diverse cell types especially in the nucleus of eukaryotic cells. PTBP1 contains four RNA recognition motifs which allow it to interact with polypyrimidine-rich sequences in pre-mRNA. This interaction plays an important role in RNA splicing and the regulation of alternative splicing events.

Biological function summary

PTBP1 influences the stability transport and translation of mRNA molecules. It is part of the heterogeneous nuclear ribonucleoprotein complex which plays a fundamental role in the processing and modification of pre-mRNA into mature mRNA. This activity impacts the expression of a wide range of genes affecting cellular function and growth. PTBP1 regulates mRNA metabolism contributing significantly to post-transcriptional gene regulation.

Pathways

The involvement of PTBP1 spans the management of alternative splicing and the modulation of gene expression. It participates importantly in the alternative splicing pathway alongside proteins like PTBP2 which perform overlapping functions in neurons. PTBP1 affects exon inclusion or exclusion influencing the genetic diversity presented in protein forms within cells. It also plays a role in the exon junction complex pathway impacting post-translational modifications and protein interactions.

Associated diseases and disorders

Variations in PTBP1 function are linked to conditions such as cancer and neurological disorders. Altered expression levels or mutations can contribute to carcinogenesis through the misregulation of splice variants specific to oncogenes and tumor suppressor genes. In neurological disorders PTBP1 interacts with proteins like FUS affecting the regulation of neuronal-specific alternative splicing. Misbalance in these interactions can have significant pathological implications contributing to diseases such as amyotrophic lateral sclerosis (ALS) and glioblastomas.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-PTBP1 antibody [EPR9048(B)] (ab133734), expandable thumbnail

    Western blot - Anti-PTBP1 antibody [EPR9048(B)] (ab133734)

    Lanes 1- 2: Merged signal (red and green). Green - ab133734 observed at 57 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.

    ab133734 was shown to react with PTBP1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line Human PTBP1 knockout HeLa cell line ab265155 (knockout cell lysate Human PTBP1 knockout HeLa cell lysate ab257614) was used. Wild-type HeLa and PTBP1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab133734 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-PTBP1 antibody [EPR9048(B)] (ab133734) at 1/10000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: PTBP1 knockout HeLa cell lysate at 20 µg

    Lane 2: Western blot - Human PTBP1 knockout HeLa cell line (Human PTBP1 knockout HeLa cell line ab265155)

    Performed under reducing conditions.

    Predicted band size: 57 kDa

    Observed band size: 57 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTBP1 antibody [EPR9048(B)] (ab133734), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTBP1 antibody [EPR9048(B)] (ab133734)

    Immunohistochemical analysis of PTBP1 in paraffin embedded Human breast carcinoma tissue stained with ab133734 at a 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-PTBP1 antibody [EPR9048(B)] (ab133734), expandable thumbnail

    Western blot - Anti-PTBP1 antibody [EPR9048(B)] (ab133734)

    Lanes 1 - 2: Merged signal (red and green). Green - ab133734 observed at 57 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.

    ab133734 was shown to recognize PTBP1 in wild-type HAP1 cells as signal was lost at the expected MW in PTBP1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and PTBP1 knockout samples were subjected to SDS-PAGE. ab133734 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-PTBP1 antibody [EPR9048(B)] (ab133734) at 1/1000 dilution

    Lane 1: Wild-type HAP1 whole cell lysate at 20 µg

    Lane 2: PTBP1 knockout HAP1 whole cell lysate at 20 µg

    Predicted band size: 57 kDa

  • Western blot - Anti-PTBP1 antibody [EPR9048(B)] (ab133734), expandable thumbnail

    Western blot - Anti-PTBP1 antibody [EPR9048(B)] (ab133734)

    All lanes: Western blot - Anti-PTBP1 antibody [EPR9048(B)] (ab133734) at 1/10000 dilution

    Lane 1: Human fetal liver lysate at 10 µg

    Lane 2: Jurkat cell lysate at 10 µg

    Lane 3: Daudi cell lysate at 10 µg

    Lane 4: A549 cell lysate at 10 µg

    Lane 5: BxPC3 cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

    Predicted band size: 57 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PTBP1 antibody [EPR9048(B)] (ab133734), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PTBP1 antibody [EPR9048(B)] (ab133734)

    Immunofluorescent staining of PTBP1 in A549 cells, using ab133734 at a 1/250 dilution.

  • Flow Cytometry (Intracellular) - Anti-PTBP1 antibody [EPR9048(B)] (ab133734), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-PTBP1 antibody [EPR9048(B)] (ab133734)

    Intracellular Flow Cytometry analysis of A549 (Human lung carcinoma epithelial cell) cells labeling PTBP1 (red) with purified ab133734 at a 1/2000 dilution (1ug/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

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Product protocols

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