Rabbit Recombinant Monoclonal PTBP1 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse samples.
Images
![Western blot - Anti-PTBP1 antibody [EPR9048(B)] - BSA and Azide free (AB240079), expandable thumbnail](https://content.abcam.com/products/images/ptbp1-antibody-epr9048b-bsa-and-azide-free-ab240079--western-blot-img1462.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PTBP1 antibody [EPR9048(B)] - BSA and Azide free (AB240079)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTBP1 antibody [EPR9048(B)] - BSA and Azide free (AB240079), expandable thumbnail](https://content.abcam.com/products/images/ptbp1-antibody-epr9048b-bsa-and-azide-free-ab240079--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img83877.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTBP1 antibody [EPR9048(B)] - BSA and Azide free (AB240079)")
![Western blot - Anti-PTBP1 antibody [EPR9048(B)] - BSA and Azide free (AB240079), expandable thumbnail](https://content.abcam.com/products/images/ptbp1-antibody-epr9048b-bsa-and-azide-free-ab240079--western-blot-img62174.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PTBP1 antibody [EPR9048(B)] - BSA and Azide free (AB240079)")
![Immunocytochemistry/ Immunofluorescence - Anti-PTBP1 antibody [EPR9048(B)] - BSA and Azide free (AB240079), expandable thumbnail](https://content.abcam.com/products/images/ptbp1-antibody-epr9048b-bsa-and-azide-free-ab240079--immunocytochemistry-immunofluorescence-img84620.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-PTBP1 antibody [EPR9048(B)] - BSA and Azide free (AB240079)")
![Flow Cytometry (Intracellular) - Anti-PTBP1 antibody [EPR9048(B)] - BSA and Azide free (AB240079), expandable thumbnail](https://content.abcam.com/products/images/ptbp1-antibody-epr9048b-bsa-and-azide-free-ab240079--flow-cytometry-intracellular-img413905.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-PTBP1 antibody [EPR9048(B)] - BSA and Azide free (AB240079)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Tested | Tested |
| Mouse | Expected | Not recommended | Expected | Expected | Expected |
| Rat | Predicted | Not recommended | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform antigen retrieval before commencing with IHC staining protocol. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform antigen retrieval before commencing with IHC staining protocol. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
8 products for Alternative Version
Target data
Function
Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Activates exon skipping of its own pre-mRNA during muscle cell differentiation. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA. Represses the splicing of MAPT/Tau exon 10 (PubMed:15009664). Binds to polypyrimidine-rich controlling element (PCE) of CFTR and promotes exon skipping of CFTR exon 9, thereby antagonizing TIA1 and its role in exon inclusion of CFTR exon 9 (PubMed:14966131). Plays a role in the splicing of pyruvate kinase PKM by binding repressively to a polypyrimidine tract flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed:20010808). In case of infection by picornaviruses, binds to the viral internal ribosome entry site (IRES) and stimulates the IRES-mediated translation (PubMed:21518806).
Alternative names
PTB, PTBP1, Polypyrimidine tract-binding protein 1, 57 kDa RNA-binding protein PPTB-1, Heterogeneous nuclear ribonucleoprotein I, hnRNP I
Recommended products
Rabbit Recombinant Monoclonal PTBP1 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR9048(B)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab240079 is the carrier-free version of Anti-PTBP1 antibody [EPR9048(B)] ab133734.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PTBP1 also known as polypyrimidine tract-binding protein 1 or hnRNP I is a protein involved in RNA binding. It weighs approximately 57 kDa and is known for its expression in diverse cell types especially in the nucleus of eukaryotic cells. PTBP1 contains four RNA recognition motifs which allow it to interact with polypyrimidine-rich sequences in pre-mRNA. This interaction plays an important role in RNA splicing and the regulation of alternative splicing events.
- Biological function summary
PTBP1 influences the stability transport and translation of mRNA molecules. It is part of the heterogeneous nuclear ribonucleoprotein complex which plays a fundamental role in the processing and modification of pre-mRNA into mature mRNA. This activity impacts the expression of a wide range of genes affecting cellular function and growth. PTBP1 regulates mRNA metabolism contributing significantly to post-transcriptional gene regulation.
- Pathways
The involvement of PTBP1 spans the management of alternative splicing and the modulation of gene expression. It participates importantly in the alternative splicing pathway alongside proteins like PTBP2 which perform overlapping functions in neurons. PTBP1 affects exon inclusion or exclusion influencing the genetic diversity presented in protein forms within cells. It also plays a role in the exon junction complex pathway impacting post-translational modifications and protein interactions.
- Associated diseases and disorders
Variations in PTBP1 function are linked to conditions such as cancer and neurological disorders. Altered expression levels or mutations can contribute to carcinogenesis through the misregulation of splice variants specific to oncogenes and tumor suppressor genes. In neurological disorders PTBP1 interacts with proteins like FUS affecting the regulation of neuronal-specific alternative splicing. Misbalance in these interactions can have significant pathological implications contributing to diseases such as amyotrophic lateral sclerosis (ALS) and glioblastomas.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-PTBP1 antibody [EPR9048(B)] - BSA and Azide free (ab240079), expandable thumbnail](https://content.abcam.com/products/images/ptbp1-antibody-epr9048b-bsa-and-azide-free-ab240079--western-blot-img1462.jpg "Western blot - Anti-PTBP1 antibody [EPR9048(B)] - BSA and Azide free (ab240079)")
Western blot - Anti-PTBP1 antibody [EPR9048(B)] - BSA and Azide free (ab240079)
This data was developed using the same antibody clone in a different buffer formulation (Anti-PTBP1 antibody [EPR9048(B)] ab133734).
Lanes 1- 2: Merged signal (red and green). Green - Anti-PTBP1 antibody [EPR9048(B)] ab133734 observed at 57 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.
Anti-PTBP1 antibody [EPR9048(B)] ab133734 was shown to react with PTBP1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line Human PTBP1 knockout HeLa cell line ab265155 (knockout cell lysate Human PTBP1 knockout HeLa cell lysate ab257614) was used. Wild-type HeLa and PTBP1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. Anti-PTBP1 antibody [EPR9048(B)] ab133734 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-PTBP1 antibody [EPR9048(B)] (Anti-PTBP1 antibody [EPR9048(B)] ab133734) at 1/10000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: PTBP1 knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human PTBP1 knockout HeLa cell line (Human PTBP1 knockout HeLa cell line ab265155)
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 57 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTBP1 antibody [EPR9048(B)] - BSA and Azide free (ab240079), expandable thumbnail](https://content.abcam.com/products/images/ptbp1-antibody-epr9048b-bsa-and-azide-free-ab240079--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img83877.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTBP1 antibody [EPR9048(B)] - BSA and Azide free (ab240079)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTBP1 antibody [EPR9048(B)] - BSA and Azide free (ab240079)
Immunohistochemical analysis of PTBP1 in paraffin embedded Human breast carcinoma tissue stained with Anti-PTBP1 antibody [EPR9048(B)] ab133734 at a 1/100 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PTBP1 antibody [EPR9048(B)] ab133734).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Western blot - Anti-PTBP1 antibody [EPR9048(B)] - BSA and Azide free (ab240079), expandable thumbnail](https://content.abcam.com/products/images/ptbp1-antibody-epr9048b-bsa-and-azide-free-ab240079--western-blot-img62174.jpg "Western blot - Anti-PTBP1 antibody [EPR9048(B)] - BSA and Azide free (ab240079)")
Western blot - Anti-PTBP1 antibody [EPR9048(B)] - BSA and Azide free (ab240079)
Lanes 1 - 2: Merged signal (red and green). Green - Anti-PTBP1 antibody [EPR9048(B)] ab133734 observed at 57 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.Anti-PTBP1 antibody [EPR9048(B)] ab133734 was shown to recognize PTBP1 in wild-type HAP1 cells as signal was lost at the expected MW in PTBP1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and PTBP1 knockout samples were subjected to SDS-PAGE. Anti-PTBP1 antibody [EPR9048(B)] ab133734 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PTBP1 antibody [EPR9048(B)] ab133734).
All lanes: Western blot - Anti-PTBP1 antibody [EPR9048(B)] (Anti-PTBP1 antibody [EPR9048(B)] ab133734) at 1/1000 dilution
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lane 2: PTBP1 knockout HAP1 whole cell lysate at 20 µg
Predicted band size: 57 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-PTBP1 antibody [EPR9048(B)] - BSA and Azide free (ab240079), expandable thumbnail](https://content.abcam.com/products/images/ptbp1-antibody-epr9048b-bsa-and-azide-free-ab240079--immunocytochemistry-immunofluorescence-img84620.jpg "Immunocytochemistry/ Immunofluorescence - Anti-PTBP1 antibody [EPR9048(B)] - BSA and Azide free (ab240079)")
Immunocytochemistry/ Immunofluorescence - Anti-PTBP1 antibody [EPR9048(B)] - BSA and Azide free (ab240079)
Immunofluorescent staining of PTBP1 in A549 cells, using Anti-PTBP1 antibody [EPR9048(B)] ab133734 at a 1/250 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PTBP1 antibody [EPR9048(B)] ab133734).
![Flow Cytometry (Intracellular) - Anti-PTBP1 antibody [EPR9048(B)] - BSA and Azide free (ab240079), expandable thumbnail](https://content.abcam.com/products/images/ptbp1-antibody-epr9048b-bsa-and-azide-free-ab240079--flow-cytometry-intracellular-img413905.jpg "Flow Cytometry (Intracellular) - Anti-PTBP1 antibody [EPR9048(B)] - BSA and Azide free (ab240079)")
Flow Cytometry (Intracellular) - Anti-PTBP1 antibody [EPR9048(B)] - BSA and Azide free (ab240079)
Intracellular Flow Cytometry analysis of A549 (Human lung carcinoma epithelial cell) cells labeling PTBP1 (red) with purified Anti-PTBP1 antibody [EPR9048(B)] ab133734 at a 1/2000 dilution (1ug/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PTBP1 antibody [EPR9048(B)] ab133734).
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