Anti-PTBP1 antibody [EPR9049(B)] - BSA and Azide free

• WB

Lab

Western blot - Anti-PTBP1 antibody [EPR9049(B)] - BSA and Azide free (AB248749)

This data was developed using the same antibody clone in a different buffer formulation (ab134950).

Lanes 1 - 2 : Merged signal (red and green). Green - ab134950 observed at 57 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab134950 was shown to specifically react with PTBP1 in wild-type HAP1 cells as signal was lost in PTBP1 knockout cells. Wild-type and PTBP1 knockout samples were subjected to SDS-PAGE. ab134950 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PTBP1 antibody [EPR9049(B)] - BSA and Azide free (ab248749) at 1000 µg

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

PTBP1 knockout HAP1 whole cell lysate at 20 µg

Predicted band size: 57 kDa

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• WB

Lab

Western blot - Anti-PTBP1 antibody [EPR9049(B)] - BSA and Azide free (AB248749)

This data was developed using the same antibody clone in a different buffer formulation (ab134950).

Lanes 1- 2 : Merged signal (red and green). Green - ab134950 observed at 57 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab134950 was shown to react with PTBP1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265155 (knockout cell lysate ab257614) was used. Wild-type HeLa and PTBP1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab134950 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PTBP1 antibody [EPR9049(B)] (<a href='/en-us/products/primary-antibodies/ptbp1-antibody-epr9049b-ab134950'>ab134950</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

PTBP1 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human PTBP1 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-ptbp1-knockout-hela-cell-line-ab265155'>ab265155</a>)

Predicted band size: 57 kDa

Observed band size: 57 kDa

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