JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB154787

Anti-PTBP2 antibody [EPR9890]

Be the first to review this product! Submit a review

|

(7 Publications)

Rabbit Recombinant Monoclonal PTBP2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Rat, Mouse samples. Cited in 7 publications.

View Alternative Names

NPTB, PTB, PTBLP, PTBP2, Polypyrimidine tract-binding protein 2, Neural polypyrimidine tract-binding protein, Neurally-enriched homolog of PTB, PTB-like protein

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTBP2 antibody [EPR9890] (AB154787)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTBP2 antibody [EPR9890] (AB154787)

Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling PTBP2 with ab154787 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-PTBP2 antibody [EPR9890] (AB154787)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PTBP2 antibody [EPR9890] (AB154787)

Immunofluorescent analysis of HeLa cells labeling PTBP2 with ab154787 at 1/250 dilution.

Western blot - Anti-PTBP2 antibody [EPR9890] (AB154787)
  • WB

Unknown

Western blot - Anti-PTBP2 antibody [EPR9890] (AB154787)

All lanes:

Western blot - Anti-PTBP2 antibody [EPR9890] (ab154787) at 1/1000 dilution

Lane 1:

C6 cell lysate at 10 µg

Lane 2:

Neuro-2a cell lysate at 10 µg

Lane 3:

SH-SY5Y cell lysate at 10 µg

Lane 4:

Y79 (Human retinoblastoma cell line) cell lysate at 10 µg

Lane 5:

HeLa cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 57 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9890

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen used for this product shares 91% homology with PTBP1 and 85% homology with PTBP3. Cross-reactivity with this protein has not been confirmed experimentally.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PTBP2 also known as Polypyrimidine Tract Binding Protein 2 or nPTB is an RNA-binding protein with an approximate molecular mass of 57 kDa. It functions mechanically through the regulation of alternative splicing a critical process in the post-transcriptional modification of pre-mRNA. PTBP2 has a prominent presence in neuronal tissues where it influences the maturation and differentiation of neurons by modulating gene expression. It shares structural similarities with its paralog PTBP1 but is expressed particularly in the nervous system.
Biological function summary

PTBP2 influences various neural functions by modulating the splicing of target RNA transcripts. It contributes to the dynamic regulation of splicing events during neurogenesis and neural development forming part of splicing regulatory networks. PTBP2 does not function as part of a larger protein complex but interacts directly with RNA substrates impacting their stability and translation efficiency. This protein is essential for specific splicing events necessary for neurodevelopmental transitions.

Pathways

PTBP2 affects neural development and differentiation through pathways like axon guidance and synaptic transmission. Its role in alternative splicing enables the proper expression of proteins involved in these pathways such as NRG1 and DSCAM. PTBP2 works closely with related splicing proteins and RNA-binding proteins coordinating the production of specific protein isoforms required for neuronal circuit formation and maintenance.

PTBP2 has connections with neurodevelopmental conditions such as autism spectrum disorders (ASD) and neurodegenerative diseases like amyotrophic lateral sclerosis (ALS). In these contexts abnormal expression or splicing activity of PTBP2 can lead to dysregulation of genes involved in neural connectivity and survival. The interactions between PTBP2 and proteins such as FUS and TDP-43 are significant in understanding its role in neurodegenerative pathways. These associations highlight the importance of precise PTBP2 function in maintaining neural health and function.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

RNA-binding protein which binds to intronic polypyrimidine tracts and mediates negative regulation of exons splicing. May antagonize in a tissue-specific manner the ability of NOVA1 to activate exon selection. In addition to its function in pre-mRNA splicing, plays also a role in the regulation of translation.. Isoform 5. Reduced affinity for RNA.
See full target information PTBP2
style
left-column

Publications (7)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:108258 PubMed38026174

2023

RBFOX2 regulated EYA3 isoforms partner with SIX4 or ZBTB1 to control transcription during myogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Hannah J Wiedner,R Eric Blue,Matheus Sadovsky,C Allie Mills,Xander H T Wehrens,Laura E Herring,Jimena Giudice

Science advances 9:eadi0286 PubMed37506203

2023

PTBP1 regulates injury responses and sensory pathways in adult peripheral neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Stefanie Alber,Pierluigi Di Matteo,Matthew D Zdradzinski,Irene Dalla Costa,Katalin F Medzihradszky,Riki Kawaguchi,Agostina Di Pizio,Philip Freund,Nicolas Panayotis,Letizia Marvaldi,Ella Doron-Mandel,Nataliya Okladnikov,Ida Rishal,Reinat Nevo,Giovanni Coppola,Seung Joon Lee,Pabitra K Sahoo,Alma L Burlingame,Jeffery L Twiss,Mike Fainzilber

RNA (New York, N.Y.) 28:523-540 PubMed35082143

2022

Alternative splicing regulation of membrane trafficking genes during myogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Emma R Hinkle,Hannah J Wiedner,Eduardo V Torres,Micaela Jackson,Adam J Black,R Eric Blue,Sarah E Harris,Bryan B Guzman,Gabrielle M Gentile,Eunice Y Lee,Yi-Hsuan Tsai,Joel Parker,Daniel Dominguez,Jimena Giudice

Cells 10: PubMed34944075

2021

hnRNP Q and hnRNP A1 Regulate the Translation of Cofilin in Response to Transient Oxygen-Glucose Deprivation in Hippocampal Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Sung Wook Kim,In Kyung Hong,Mingee Kim,Yun Seon Song,Kyong-Tai Kim

Frontiers in genetics 12:752495 PubMed34707642

2021

A Novel Meiosis-Related lncRNA, Rbakdn, Contributes to Spermatogenesis by Stabilizing Ptbp2.

Applications

Unspecified application

Species

Unspecified reactive species

Wensheng Liu,Yinan Zhao,Xiaohua Liu,Xiaoya Zhang,Jiancheng Ding,Yang Li,Yingpu Tian,Haibin Wang,Wen Liu,Zhongxian Lu

Molecular therapy. Nucleic acids 11:79-90 PubMed29858092

2018

Supplemental Treatment for Huntington's Disease with miR-132 that Is Deficient in Huntington's Disease Brain.

Applications

Unspecified application

Species

Unspecified reactive species

Masashi Fukuoka,Masaki Takahashi,Hiromi Fujita,Tomoko Chiyo,H Akiko Popiel,Shoko Watanabe,Hirokazu Furuya,Miho Murata,Keiji Wada,Takashi Okada,Yoshitaka Nagai,Hirohiko Hohjoh

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 29:1113-23 PubMed25466893

2014

Hepatitis B virus core protein inhibits Fas-mediated apoptosis of hepatoma cells via regulation of mFas/FasL and sFas expression.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Liu,Yan-Ting Lin,Xiao-Li Yan,Ya-Lan Ding,Yun-Li Wu,Wan-Nan Chen,Xu Lin
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB154853

Anti-PTBP2 antibody [EPR9891]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-PTBP2 antibody [EPR9891] (AB154853)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB133734

Anti-PTBP1 antibody [EPR9048(B)]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTBP1 antibody [EPR9048(B)] (AB133734)

  • 0
  • 0
Primary Antibodies

AB1791

Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade

Lab Essentials

ChIP - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)

  • 5
  • 233
Primary Antibodies

AB18207

Anti-beta III Tubulin antibody - Neuronal Marker

BOND RX™ Validated|Lab Essentials|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody - Neuronal Marker (AB18207)

  • 5
  • 67
Primary Antibodies

AB207176

Anti-Neurofilament heavy polypeptide antibody [EPR20020]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (AB207176)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com