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Rabbit Recombinant Monoclonal PTBP2 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Rat, Mouse samples.

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Images

Western blot - Anti-PTBP2 antibody [EPR9890] - BSA and Azide free (AB249098), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTBP2 antibody [EPR9890] - BSA and Azide free (AB249098), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PTBP2 antibody [EPR9890] - BSA and Azide free (AB249098), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFIPFlow CytWB
Human
Tested
Tested
Not recommended
Not recommended
Tested
Mouse
Expected
Expected
Not recommended
Not recommended
Tested
Rat
Expected
Expected
Not recommended
Not recommended
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species

Rat, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Rat, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Rat, Human, Mouse

Dilution info

-

Notes

-

Target data

Function

RNA-binding protein which binds to intronic polypyrimidine tracts and mediates negative regulation of exons splicing. May antagonize in a tissue-specific manner the ability of NOVA1 to activate exon selection. In addition to its function in pre-mRNA splicing, plays also a role in the regulation of translation. Isoform 5 has a reduced affinity for RNA.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal PTBP2 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Rat, Mouse samples.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR9890

Purification technique

Affinity purification

Specificity

The immunogen used for this product shares 91% homology with PTBP1 and 85% homology with PTBP3. Cross-reactivity with this protein has not been confirmed experimentally.

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab249098 is the carrier-free version of Anti-PTBP2 antibody [EPR9890] ab154787.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

PTBP2 also known as Polypyrimidine Tract Binding Protein 2 or nPTB is an RNA-binding protein with an approximate molecular mass of 57 kDa. It functions mechanically through the regulation of alternative splicing a critical process in the post-transcriptional modification of pre-mRNA. PTBP2 has a prominent presence in neuronal tissues where it influences the maturation and differentiation of neurons by modulating gene expression. It shares structural similarities with its paralog PTBP1 but is expressed particularly in the nervous system.

Biological function summary

PTBP2 influences various neural functions by modulating the splicing of target RNA transcripts. It contributes to the dynamic regulation of splicing events during neurogenesis and neural development forming part of splicing regulatory networks. PTBP2 does not function as part of a larger protein complex but interacts directly with RNA substrates impacting their stability and translation efficiency. This protein is essential for specific splicing events necessary for neurodevelopmental transitions.

Pathways

PTBP2 affects neural development and differentiation through pathways like axon guidance and synaptic transmission. Its role in alternative splicing enables the proper expression of proteins involved in these pathways such as NRG1 and DSCAM. PTBP2 works closely with related splicing proteins and RNA-binding proteins coordinating the production of specific protein isoforms required for neuronal circuit formation and maintenance.

Associated diseases and disorders

PTBP2 has connections with neurodevelopmental conditions such as autism spectrum disorders (ASD) and neurodegenerative diseases like amyotrophic lateral sclerosis (ALS). In these contexts abnormal expression or splicing activity of PTBP2 can lead to dysregulation of genes involved in neural connectivity and survival. The interactions between PTBP2 and proteins such as FUS and TDP-43 are significant in understanding its role in neurodegenerative pathways. These associations highlight the importance of precise PTBP2 function in maintaining neural health and function.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-PTBP2 antibody [EPR9890] - BSA and Azide free (ab249098), expandable thumbnail

    Western blot - Anti-PTBP2 antibody [EPR9890] - BSA and Azide free (ab249098)

    This data was developed using Anti-PTBP2 antibody [EPR9890] ab154787, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-PTBP2 antibody [EPR9890] (Anti-PTBP2 antibody [EPR9890] ab154787) at 1/1000 dilution

    Lane 1: C6 cell lysate at 10 µg

    Lane 2: Neuro-2a cell lysate at 10 µg

    Lane 3: SH-SY5Y cell lysate at 10 µg

    Lane 4: Y79 (Human retinoblastoma cell line) cell lysate at 10 µg

    Lane 5: HeLa cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 57 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTBP2 antibody [EPR9890] - BSA and Azide free (ab249098), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTBP2 antibody [EPR9890] - BSA and Azide free (ab249098)

    This data was developed using Anti-PTBP2 antibody [EPR9890] ab154787, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling PTBP2 with Anti-PTBP2 antibody [EPR9890] ab154787 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-PTBP2 antibody [EPR9890] - BSA and Azide free (ab249098), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PTBP2 antibody [EPR9890] - BSA and Azide free (ab249098)

    This data was developed using Anti-PTBP2 antibody [EPR9890] ab154787, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells labeling PTBP2 with Anti-PTBP2 antibody [EPR9890] ab154787 at 1/250 dilution.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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