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AB249155

Anti-PTBP2 antibody [EPR9891] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal PTBP2 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 1 publication.

View Alternative Names

NPTB, PTB, PTBLP, PTBP2, Polypyrimidine tract-binding protein 2, Neural polypyrimidine tract-binding protein, Neurally-enriched homolog of PTB, PTB-like protein

1 Images
Western blot - Anti-PTBP2 antibody [EPR9891] - BSA and Azide free (AB249155)
  • WB

Unknown

Western blot - Anti-PTBP2 antibody [EPR9891] - BSA and Azide free (AB249155)

This data was developed using ab154853, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PTBP2 antibody [EPR9891] (<a href='/en-us/products/primary-antibodies/ptbp2-antibody-epr9891-ab154853'>ab154853</a>) at 1/1000 dilution

Lane 1:

Human fetal brain lysate at 10 µg

Lane 2:

Y79 cell lysate at 10 µg

Lane 3:

Neuro 2a cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 57 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9891

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen used for this product shares 93% homology with PTBP1 and PTBP3. Cross-reactivity with this protein has not been confirmed experimentally.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab249155 is the carrier-free version of ab154853.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PTBP2 also known as Polypyrimidine Tract Binding Protein 2 or nPTB is an RNA-binding protein with an approximate molecular mass of 57 kDa. It functions mechanically through the regulation of alternative splicing a critical process in the post-transcriptional modification of pre-mRNA. PTBP2 has a prominent presence in neuronal tissues where it influences the maturation and differentiation of neurons by modulating gene expression. It shares structural similarities with its paralog PTBP1 but is expressed particularly in the nervous system.
Biological function summary

PTBP2 influences various neural functions by modulating the splicing of target RNA transcripts. It contributes to the dynamic regulation of splicing events during neurogenesis and neural development forming part of splicing regulatory networks. PTBP2 does not function as part of a larger protein complex but interacts directly with RNA substrates impacting their stability and translation efficiency. This protein is essential for specific splicing events necessary for neurodevelopmental transitions.

Pathways

PTBP2 affects neural development and differentiation through pathways like axon guidance and synaptic transmission. Its role in alternative splicing enables the proper expression of proteins involved in these pathways such as NRG1 and DSCAM. PTBP2 works closely with related splicing proteins and RNA-binding proteins coordinating the production of specific protein isoforms required for neuronal circuit formation and maintenance.

PTBP2 has connections with neurodevelopmental conditions such as autism spectrum disorders (ASD) and neurodegenerative diseases like amyotrophic lateral sclerosis (ALS). In these contexts abnormal expression or splicing activity of PTBP2 can lead to dysregulation of genes involved in neural connectivity and survival. The interactions between PTBP2 and proteins such as FUS and TDP-43 are significant in understanding its role in neurodegenerative pathways. These associations highlight the importance of precise PTBP2 function in maintaining neural health and function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

RNA-binding protein which binds to intronic polypyrimidine tracts and mediates negative regulation of exons splicing. May antagonize in a tissue-specific manner the ability of NOVA1 to activate exon selection. In addition to its function in pre-mRNA splicing, plays also a role in the regulation of translation.. Isoform 5. Reduced affinity for RNA.
See full target information PTBP2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Developmental cell 60:2744-2760.e9 PubMed40460835

2025

ATG9A and ARFIP2 cooperate to control PI4P levels for lysosomal repair.

Applications

Unspecified application

Species

Unspecified reactive species

Stefano De Tito,Eugenia Almacellas,Daniel Dai Yu,Emily Millard,Wenxin Zhang,Cecilia de Heus,Christophe Queval,Javier H Hervás,Enrica Pellegrino,Ioanna Panagi,Ditte Fogde,Teresa L M Thurston,Judith Klumperman,Maximiliano Gutierrez,Sharon A Tooze
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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