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AB238338

Anti-PTCH2 antibody

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(2 Publications)

Rabbit Polyclonal PTCH2 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PTCH2 aa 750-1000.

View Alternative Names

UNQ560/PRO1121/PRO57079, PTCH2, Protein patched homolog 2, PTC2

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-PTCH2 antibody (AB238338)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PTCH2 antibody (AB238338)

HEK-293 (Human epithelial cell line from embryonic kidney) cells stained for PTCH2 (green) using ab238338 at 1/66 dilution in ICC/IF. Secondary antibody is an Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L).

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTCH2 antibody (AB238338)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTCH2 antibody (AB238338)

Paraffin-embedded human brain tissue stained for PTCH2 using ab238338 at 1/200 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at room temperature. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human PTCH2 aa 750-1000. The exact immunogen used to generate this antibody is proprietary information.

Q9Y6C5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PTCH2 also known as Patched 2 is a protein with a mass of approximately 145 kDa. It is a member of the Patched family of proteins and functions as a transmembrane receptor. PTCH2 is predominantly found on the surface of cells in tissues such as skin brain and testes. The protein serves an important role in regulating the signaling of the Hedgehog pathway by binding to its ligand Sonic Hedgehog which controls the activity of the smoothened (SMO) receptor.
Biological function summary

PTCH2 participates in the negative regulation of the Hedgehog signaling pathway. It forms a receptor complex with other proteins to maintain proper cell communication and growth control. PTCH2 is part of a larger complex that includes Patched 1 (PTCH1) contributing to embryonic development and tissue patterning. Its regulation of cellular growth and differentiation processes is critical for these activities.

Pathways

PTCH2 is a critical component of the Hedgehog signaling pathway and the development pathway. It closely interacts with PTCH1 and SMO in regulating signal transduction. Communication within these pathways is essential for tissue patterning and organogenesis. PTCH2's modulation of SMO results in downstream effects which impact the transcription of target genes controlling cell proliferation.

PTCH2 has a link to basal cell carcinoma and medulloblastoma. Its aberrant signaling and mutations may lead to uncontrolled cellular proliferation contributing to the onset of these cancers. The protein's interaction with PTCH1 also affects cancer susceptibility since both proteins can influence the progression and risk of developing tumors associated with the Hedgehog pathway.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in the control of cellular growth (PubMed : 18285427). May have a role in epidermal development. May act as a receptor for Sonic hedgehog (SHH).
See full target information PTCH2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 29:15 PubMed39492940

2024

UL16‑binding protein 1 is a significant prognostic and diagnostic marker for breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaowei Zhang,Shuhong Dai,Liang Li,Pengyun Wang,Mingxin Dong

PloS one 14:e0225511 PubMed31756206

2019

Expression profile of sonic hedgehog signaling-related molecules in basal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hye Sung Kim,Young Sil Kim,Chul Lee,Myung Soo Shin,Jae Wang Kim,Bo Gun Jang
View all publications
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