Goat Polyclonal PTCHD1 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human PTCHD1 aa 250-300.
Images
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human PTCHD1 aa 250-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q96NR3
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
| Chicken | Predicted |
| Cow | Predicted |
| Dog | Predicted |
| Pig | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.50000-2.00000 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Cow, Dog, Pig | Dilution info - | Notes - |
Target data
Function
Required for the development and function of the thalamic reticular nucleus (TRN), a part of the thalamus that is critical for thalamocortical transmission, generation of sleep rhythms, sensorimotor processing and attention. Can bind cholesterol in vitro (PubMed:36769003).
Alternative names
Patched domain-containing protein 1, PTCHD1
Recommended products
Goat Polyclonal PTCHD1 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human PTCHD1 aa 250-300.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human PTCHD1 aa 250-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q96NR3
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab109407 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PTCHD1 also known as Patched domain-containing protein 1 is a protein with a mass of approximately 139 kDa. This protein functions as a receptor that interacts with and regulates the Hedgehog signaling pathway. PTCHD1 is widely expressed in the human brain particularly in both neuronal and glial cells. This expression pattern suggests a significant role in neural development and function. The protein structure includes a large extracellular domain allowing it to bind with various ligands associated with cell signaling processes.
- Biological function summary
PTCHD1 plays an important role in modulating the Hedgehog signaling pathway which is essential for cell differentiation and growth. This protein is thought to work as a part of a complex involving the regulation of cell membrane-associated signaling proteins. Its presence ensures correct signaling dynamics influencing cellular processes such as proliferation and tissue patterning during development. PTCHD1's function highlights its potential impact on the proper organization and operation of the neural environments.
- Pathways
PTCHD1 is significantly involved in the Hedgehog signaling and its regulation. This pathway is important for embryonic development and various postnatal processes. PTCHD1 interacts with other proteins in this pathway such as Smoothened (SMO) and the GLI family of transcription factors coordinating the transmission of signals that govern cellular fate decisions. Through these interactions PTCHD1 influences processes from growth and differentiation to apoptosis.
- Associated diseases and disorders
Alterations in PTCHD1 expression or function relate to neurodevelopmental disorders such as autism spectrum disorders (ASD) and intellectual disabilities. Studies have shown that PTCHD1 mutations can disrupt normal Hedgehog signaling influencing brain development and function. Additionally PTCHD1's interaction with other proteins like SHANK3 highlights its significance in maintaining synaptic integrity and function further associating it with ASD and related conditions. Understanding these connections can aid in developing targeted therapies for these complex disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-PTCHD1 antibody (ab109407)
An additional band of unknown identity was also consistently observed at 50kDa. This band was successfully blocked by incubation with the immunizing peptide.
All lanes: Western blot - Anti-PTCHD1 antibody (ab109407) at 0.5 µg/mL
All lanes: Human Brain Cerebellum lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 101 kDa
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