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AB109407

Anti-PTCHD1 antibody

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(1 Review)

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(3 Publications)

Goat Polyclonal PTCHD1 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human PTCHD1 aa 250-300.

View Alternative Names

Patched domain-containing protein 1, PTCHD1

1 Images
Western blot - Anti-PTCHD1 antibody (AB109407)
  • WB

Unknown

Western blot - Anti-PTCHD1 antibody (AB109407)

An additional band of unknown identity was also consistently observed at 50kDa. This band was successfully blocked by incubation with the immunizing peptide.

All lanes:

Western blot - Anti-PTCHD1 antibody (ab109407) at 0.5 µg/mL

All lanes:

Human Brain Cerebellum lysate in RIPA buffer at 35 µg

Predicted band size: 101 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human PTCHD1 aa 250-300. The exact immunogen used to generate this antibody is proprietary information.

Q96NR3

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab109407 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PTCHD1 also known as Patched domain-containing protein 1 is a protein with a mass of approximately 139 kDa. This protein functions as a receptor that interacts with and regulates the Hedgehog signaling pathway. PTCHD1 is widely expressed in the human brain particularly in both neuronal and glial cells. This expression pattern suggests a significant role in neural development and function. The protein structure includes a large extracellular domain allowing it to bind with various ligands associated with cell signaling processes.
Biological function summary

PTCHD1 plays an important role in modulating the Hedgehog signaling pathway which is essential for cell differentiation and growth. This protein is thought to work as a part of a complex involving the regulation of cell membrane-associated signaling proteins. Its presence ensures correct signaling dynamics influencing cellular processes such as proliferation and tissue patterning during development. PTCHD1's function highlights its potential impact on the proper organization and operation of the neural environments.

Pathways

PTCHD1 is significantly involved in the Hedgehog signaling and its regulation. This pathway is important for embryonic development and various postnatal processes. PTCHD1 interacts with other proteins in this pathway such as Smoothened (SMO) and the GLI family of transcription factors coordinating the transmission of signals that govern cellular fate decisions. Through these interactions PTCHD1 influences processes from growth and differentiation to apoptosis.

Alterations in PTCHD1 expression or function relate to neurodevelopmental disorders such as autism spectrum disorders (ASD) and intellectual disabilities. Studies have shown that PTCHD1 mutations can disrupt normal Hedgehog signaling influencing brain development and function. Additionally PTCHD1's interaction with other proteins like SHANK3 highlights its significance in maintaining synaptic integrity and function further associating it with ASD and related conditions. Understanding these connections can aid in developing targeted therapies for these complex disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for the development and function of the thalamic reticular nucleus (TRN), a part of the thalamus that is critical for thalamocortical transmission, generation of sleep rhythms, sensorimotor processing and attention. Can bind cholesterol in vitro (PubMed : 36769003).
See full target information PTCHD1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell stem cell 30:677-688.e5 PubMed37019105

2023

Generation of ventralized human thalamic organoids with thalamic reticular nucleus.

Applications

Unspecified application

Species

Unspecified reactive species

Ferdi Ridvan Kiral,Bilal Cakir,Yoshiaki Tanaka,Jonghun Kim,Woo Sub Yang,Fabien Wehbe,Young-Jin Kang,Mei Zhong,Gizem Sancer,Sang-Hun Lee,Yangfei Xiang,In-Hyun Park

Stem cells international 2023:1662182 PubMed39280589

2023

Hypoxia-Elicited Mesenchymal Stem Cell-Derived Small Extracellular Vesicles Alleviate Myocardial Infarction by Promoting Angiogenesis through the miR-214/Sufu Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lianbo Shao,Yihuan Chen,Jingjing Li,Jingfan Chao,Ziying Yang,Yinglong Ding,Han Shen,Yueqiu Chen,Zhenya Shen

Nature communications 11:2588 PubMed32444594

2020

KAT3-dependent acetylation of cell type-specific genes maintains neuronal identity in the adult mouse brain.

Applications

Unspecified application

Species

Unspecified reactive species

Michal Lipinski,Rafael Muñoz-Viana,Beatriz Del Blanco,Angel Marquez-Galera,Juan Medrano-Relinque,José M Caramés,Andrzej A Szczepankiewicz,Jordi Fernandez-Albert,Carmen M Navarrón,Roman Olivares,Grzegorz M Wilczyński,Santiago Canals,Jose P Lopez-Atalaya,Angel Barco
View all publications
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