Rabbit Recombinant Monoclonal PTDSS1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Liquid
Monoclonal
IP | WB | ICC/IF | IHC-P | Flow Cyt | |
---|---|---|---|---|---|
Human | Tested | Tested | Not recommended | Not recommended | Not recommended |
Mouse | Expected | Tested | Not recommended | Not recommended | Not recommended |
Rat | Predicted | Predicted | Not recommended | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes The observed band size of ab157222 is 42kda, this is attributed to the high content of hydrophobic amino acids in PSS1 (PMID: 8898108 and PMID: 10938271). |
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes The observed band size of ab157222 is 42kda, this is attributed to the high content of hydrophobic amino acids in PSS1 (PMID: 8898108 and PMID: 10938271). |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
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Catalyzes a base-exchange reaction in which the polar head group of phosphatidylethanolamine (PE) or phosphatidylcholine (PC) is replaced by L-serine (PubMed:19014349, PubMed:24241535). Catalyzes mainly the conversion of phosphatidylcholine (PubMed:19014349, PubMed:24241535). Also converts, in vitro and to a lesser extent, phosphatidylethanolamine (PubMed:19014349, PubMed:24241535).
KIAA0024, PSSA, PTDSS1, PSSA, KIAA0024, Phosphatidylserine synthase 1, PSS-1, PtdSer synthase 1, Serine-exchange enzyme I
Rabbit Recombinant Monoclonal PTDSS1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Liquid
Monoclonal
EPR8118(2)
Affinity purification Protein A
Blue Ice
+4°C
-20°C
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This supplementary information is collated from multiple sources and compiled automatically.
PTDSS1 also known as phosphatidylserine synthase 1 catalyzes the conversion of phosphatidylcholine to phosphatidylserine. This enzyme has a molecular mass of approximately 55 kDa and predominantly resides in the endoplasmic reticulum. It plays a role in lipid metabolism and is expressed in various tissues but is particularly active in the brain and liver. The activity of PTDSS1 is essential for maintaining the balance between different phospholipids in cellular membranes.
Phosphatidylserine synthase 1 contributes to the production of phosphatidylserine an important phospholipid found in cell membranes and involved in cell signaling and apoptosis. PTDSS1 is not part of any known complex but regulates phosphatidylserine levels that aid in cellular functions such as lipid signaling and membrane structure. This regulation is essential as disruptions can influence cell function and viability.
PTDSS1 is involved in the phospholipid biosynthesis pathway which is important for maintaining the function of cellular membranes. It influences pathways associated with cell survival and apoptosis due to its role in balancing phospholipid composition. PTDSS1 interplays with other proteins in these pathways including kinases and phosphatases which modulate cell signaling and membrane dynamics.
PTDSS1 alterations have connections to conditions such as neurological disorders and metabolic syndromes. Disruption in phosphatidylserine levels can contribute to neurodegenerative diseases where PTDSS1 interacts with proteins involved in cell death and survival pathways. Additionally abnormalities in PTDSS1 expression or function might link to metabolic disorders affecting lipid metabolism and cellular energy balance.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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Terms & Conditions.
The observed band size of ab157222 is 42kda, this is attributed to the high content of hydrophobic amino acids in PSS1 (PMID: 8898108 and PMID: 10938271).
All lanes: Western blot - Anti-PTDSS1 antibody [EPR8118(2)] (ab157222) at 1/1000 dilution
Lane 1: Human fetal liver tissue lysate at 10 µg
Lane 2: RAW 264.7 cell lysate at 10 µg
Lane 3: NIH/3T3 cell lysate at 10 µg
Lane 4: Human fetal brain tissue lysate at 10 µg
Lane 5: A549 cell lysate at 10 µg
Lane 6: 293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg
All lanes: Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 56 kDa
Immunoprecipitation : 293T cell lysate immunoprecipitated with ab157222 at 1/10
Western Blot: ab157222 at 1/1000 labeling PTDSS1 in immunoprecipitation pellet from 293T cell lysate
Secondary Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size : 56 kDa
The observed band size of ab157222 is 42kda, this is attributed to the high content of hydrophobic amino acids in PSS1 (PMID: 8898108 and PMID: 10938271).
All lanes: Immunoprecipitation - Anti-PTDSS1 antibody [EPR8118(2)] (ab157222)
Predicted band size: 56 kDa
Observed band size: 17 kDa (Recombinant protein fragment)
Blocking buffer and concentration 5% NFDM/TBST
Diluting buffer and concentration 5% NFDM /TBST
All lanes: Western blot - Anti-PTDSS1 antibody [EPR8118(2)] (ab157222) at 1/1000 dilution
All lanes: His-tagged human PTDSS1 (aa2-35-GGGGS-aa2-35-GGGGS-aa2-35) recombinant protein 15ng
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 56 kDa
Exposure time: 1s
This data was developed using ab157222, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Observed band size: 17 kDa (Recombinant protein fragment).
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