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Rabbit Recombinant Monoclonal PTDSS1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications.

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Images

Western blot - Anti-PTDSS1 antibody [EPR8118(2)] (AB157222), expandable thumbnail
  • Immunoprecipitation - Anti-PTDSS1 antibody [EPR8118(2)] (AB157222), expandable thumbnail
  • Western blot - Anti-PTDSS1 antibody [EPR8118(2)] (AB157222), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFIHC-PFlow Cyt
Human
Tested
Tested
Not recommended
Not recommended
Not recommended
Mouse
Expected
Tested
Not recommended
Not recommended
Not recommended
Rat
Predicted
Predicted
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

1/10 - 1/100

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000 - 1/10000

Notes

The observed band size of ab157222 is 42kda, this is attributed to the high content of hydrophobic amino acids in PSS1 (PMID: 8898108 and PMID: 10938271).

Species

Mouse

Dilution info

1/1000 - 1/10000

Notes

The observed band size of ab157222 is 42kda, this is attributed to the high content of hydrophobic amino acids in PSS1 (PMID: 8898108 and PMID: 10938271).

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Human, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Catalyzes a base-exchange reaction in which the polar head group of phosphatidylethanolamine (PE) or phosphatidylcholine (PC) is replaced by L-serine (PubMed:19014349, PubMed:24241535). Catalyzes mainly the conversion of phosphatidylcholine (PubMed:19014349, PubMed:24241535). Also converts, in vitro and to a lesser extent, phosphatidylethanolamine (PubMed:19014349, PubMed:24241535).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal PTDSS1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR8118(2)

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

PTDSS1 also known as phosphatidylserine synthase 1 catalyzes the conversion of phosphatidylcholine to phosphatidylserine. This enzyme has a molecular mass of approximately 55 kDa and predominantly resides in the endoplasmic reticulum. It plays a role in lipid metabolism and is expressed in various tissues but is particularly active in the brain and liver. The activity of PTDSS1 is essential for maintaining the balance between different phospholipids in cellular membranes.

Biological function summary

Phosphatidylserine synthase 1 contributes to the production of phosphatidylserine an important phospholipid found in cell membranes and involved in cell signaling and apoptosis. PTDSS1 is not part of any known complex but regulates phosphatidylserine levels that aid in cellular functions such as lipid signaling and membrane structure. This regulation is essential as disruptions can influence cell function and viability.

Pathways

PTDSS1 is involved in the phospholipid biosynthesis pathway which is important for maintaining the function of cellular membranes. It influences pathways associated with cell survival and apoptosis due to its role in balancing phospholipid composition. PTDSS1 interplays with other proteins in these pathways including kinases and phosphatases which modulate cell signaling and membrane dynamics.

Associated diseases and disorders

PTDSS1 alterations have connections to conditions such as neurological disorders and metabolic syndromes. Disruption in phosphatidylserine levels can contribute to neurodegenerative diseases where PTDSS1 interacts with proteins involved in cell death and survival pathways. Additionally abnormalities in PTDSS1 expression or function might link to metabolic disorders affecting lipid metabolism and cellular energy balance.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-PTDSS1 antibody [EPR8118(2)] (ab157222), expandable thumbnail

    Western blot - Anti-PTDSS1 antibody [EPR8118(2)] (ab157222)

    The observed band size of ab157222 is 42kda, this is attributed to the high content of hydrophobic amino acids in PSS1 (PMID: 8898108 and PMID: 10938271).

    All lanes: Western blot - Anti-PTDSS1 antibody [EPR8118(2)] (ab157222) at 1/1000 dilution

    Lane 1: Human fetal liver tissue lysate at 10 µg

    Lane 2: RAW 264.7 cell lysate at 10 µg

    Lane 3: NIH/3T3 cell lysate at 10 µg

    Lane 4: Human fetal brain tissue lysate at 10 µg

    Lane 5: A549 cell lysate at 10 µg

    Lane 6: 293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

    Predicted band size: 56 kDa

  • Immunoprecipitation - Anti-PTDSS1 antibody [EPR8118(2)] (ab157222), expandable thumbnail

    Immunoprecipitation - Anti-PTDSS1 antibody [EPR8118(2)] (ab157222)

    Immunoprecipitation : 293T cell lysate immunoprecipitated with ab157222 at 1/10

    Western Blot: ab157222 at 1/1000 labeling PTDSS1 in immunoprecipitation pellet from 293T cell lysate

    Secondary Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

    Predicted band size : 56 kDa

    The observed band size of ab157222 is 42kda, this is attributed to the high content of hydrophobic amino acids in PSS1 (PMID: 8898108 and PMID: 10938271).

    All lanes: Immunoprecipitation - Anti-PTDSS1 antibody [EPR8118(2)] (ab157222)

    Predicted band size: 56 kDa

  • Western blot - Anti-PTDSS1 antibody [EPR8118(2)] (ab157222), expandable thumbnail

    Western blot - Anti-PTDSS1 antibody [EPR8118(2)] (ab157222)

    Observed band size: 17 kDa (Recombinant protein fragment)

    Blocking buffer and concentration 5% NFDM/TBST
    Diluting buffer and concentration 5% NFDM /TBST

    All lanes: Western blot - Anti-PTDSS1 antibody [EPR8118(2)] (ab157222) at 1/1000 dilution

    All lanes: His-tagged human PTDSS1 (aa2-35-GGGGS-aa2-35-GGGGS-aa2-35) recombinant protein 15ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 56 kDa

    Exposure time: 1s

    This data was developed using ab157222, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Observed band size: 17 kDa (Recombinant protein fragment).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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