JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB157222

Anti-PTDSS1 antibody [EPR8118(2)]

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Recombinant Monoclonal PTDSS1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications.

View Alternative Names

KIAA0024, PSSA, PTDSS1, Phosphatidylserine synthase 1, PSS-1, PtdSer synthase 1, Serine-exchange enzyme I

3 Images
Immunoprecipitation - Anti-PTDSS1 antibody [EPR8118(2)] (AB157222)
  • IP

Unknown

Immunoprecipitation - Anti-PTDSS1 antibody [EPR8118(2)] (AB157222)

Immunoprecipitation : 293T cell lysate immunoprecipitated with ab157222 at 1/10

Western Blot : ab157222 at 1/1000 labeling PTDSS1 in immunoprecipitation pellet from 293T cell lysate

Secondary Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size : 56 kDa

The observed band size of ab157222 is 42kda, this is attributed to the high content of hydrophobic amino acids in PSS1 (PMID : 8898108 and PMID : 10938271).

All lanes:

Immunoprecipitation - Anti-PTDSS1 antibody [EPR8118(2)] (ab157222)

Predicted band size: 56 kDa

false

Western blot - Anti-PTDSS1 antibody [EPR8118(2)] (AB157222)
  • WB

Unknown

Western blot - Anti-PTDSS1 antibody [EPR8118(2)] (AB157222)

The observed band size of ab157222 is 42kda, this is attributed to the high content of hydrophobic amino acids in PSS1 (PMID : 8898108 and PMID : 10938271).

All lanes:

Western blot - Anti-PTDSS1 antibody [EPR8118(2)] (ab157222) at 1/1000 dilution

Lane 1:

Human fetal liver tissue lysate at 10 µg

Lane 2:

RAW 264.7 cell lysate at 10 µg

Lane 3:

NIH/3T3 cell lysate at 10 µg

Lane 4:

Human fetal brain tissue lysate at 10 µg

Lane 5:

A549 cell lysate at 10 µg

Lane 6:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 56 kDa

false

Western blot - Anti-PTDSS1 antibody [EPR8118(2)] (AB157222)
  • WB

Lab

Western blot - Anti-PTDSS1 antibody [EPR8118(2)] (AB157222)

Observed band size : 17 kDa (Recombinant protein fragment)

Blocking buffer and concentration 5% NFDM/TBST
Diluting buffer and concentration 5% NFDM /TBST

All lanes:

Western blot - Anti-PTDSS1 antibody [EPR8118(2)] (ab157222) at 1/1000 dilution

All lanes:

His-tagged human PTDSS1 (aa2-35-GGGGS-aa2-35-GGGGS-aa2-35) recombinant protein 15ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 56 kDa

false

Exposure time: 1s

  • Carrier free

    Anti-PTDSS1 antibody [EPR8118(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8118(2)

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p>The observed band size of ab157222 is 42kda, this is attributed to the high content of hydrophobic amino acids in PSS1 (PMID: 8898108 and PMID: 10938271).</p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p>The observed band size of ab157222 is 42kda, this is attributed to the high content of hydrophobic amino acids in PSS1 (PMID: 8898108 and PMID: 10938271).</p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }
style
left-column
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PTDSS1 also known as phosphatidylserine synthase 1 catalyzes the conversion of phosphatidylcholine to phosphatidylserine. This enzyme has a molecular mass of approximately 55 kDa and predominantly resides in the endoplasmic reticulum. It plays a role in lipid metabolism and is expressed in various tissues but is particularly active in the brain and liver. The activity of PTDSS1 is essential for maintaining the balance between different phospholipids in cellular membranes.
Biological function summary

Phosphatidylserine synthase 1 contributes to the production of phosphatidylserine an important phospholipid found in cell membranes and involved in cell signaling and apoptosis. PTDSS1 is not part of any known complex but regulates phosphatidylserine levels that aid in cellular functions such as lipid signaling and membrane structure. This regulation is essential as disruptions can influence cell function and viability.

Pathways

PTDSS1 is involved in the phospholipid biosynthesis pathway which is important for maintaining the function of cellular membranes. It influences pathways associated with cell survival and apoptosis due to its role in balancing phospholipid composition. PTDSS1 interplays with other proteins in these pathways including kinases and phosphatases which modulate cell signaling and membrane dynamics.

PTDSS1 alterations have connections to conditions such as neurological disorders and metabolic syndromes. Disruption in phosphatidylserine levels can contribute to neurodegenerative diseases where PTDSS1 interacts with proteins involved in cell death and survival pathways. Additionally abnormalities in PTDSS1 expression or function might link to metabolic disorders affecting lipid metabolism and cellular energy balance.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Catalyzes a base-exchange reaction in which the polar head group of phosphatidylethanolamine (PE) or phosphatidylcholine (PC) is replaced by L-serine (PubMed : 19014349, PubMed : 24241535). Catalyzes mainly the conversion of phosphatidylcholine (PubMed : 19014349, PubMed : 24241535). Also converts, in vitro and to a lesser extent, phosphatidylethanolamine (PubMed : 19014349, PubMed : 24241535).
See full target information PTDSS1
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of cell biology 223: PubMed38048228

2023

Phosphatidylserine synthesis controls oncogenic B cell receptor signaling in B cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jumpei Omi,Taiga Kato,Yohei Yoshihama,Koki Sawada,Nozomu Kono,Junken Aoki

Cell stem cell 24:621-636.e16 PubMed30930145

2019

The Mitochondrial Transacylase, Tafazzin, Regulates for AML Stemness by Modulating Intracellular Levels of Phospholipids.

Applications

Unspecified application

Species

Unspecified reactive species

Ayesh K Seneviratne,Mingjing Xu,Juan J Aristizabal Henao,Val A Fajardo,Zhenyue Hao,Veronique Voisin,G Wei Xu,Rose Hurren,S Kim,Neil MacLean,Xiaoming Wang,Marcela Gronda,Danny Jeyaraju,Yulia Jitkova,Troy Ketela,Michael Mullokandov,David Sharon,Geethu Thomas,Raphaël Chouinard-Watkins,James R Hawley,Caitlin Schafer,Helen Loo Yau,Zaza Khuchua,Ahmed Aman,Rima Al-Awar,Atan Gross,Steven M Claypool,Richard P Bazinet,Mathieu Lupien,Steven Chan,Daniel D De Carvalho,Mark D Minden,Gary D Bader,Ken D Stark,Paul LeBlanc,Aaron D Schimmer
View all publications
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
style
right-column

Associated Products

Select an associated product type
Alternative Product
Primary Antibodies

AB237019

Anti-PTDSS1 antibody

primary-antibodies

ptdss1-antibody-ab237019

0

(0 reviews)

style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com