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AB249322

Anti-PTDSS1 antibody [EPR8118(2)] - BSA and Azide free

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Rabbit Recombinant Monoclonal PTDSS1 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Recombinant fragment - Human samples.

View Alternative Names

KIAA0024, PSSA, PTDSS1, Phosphatidylserine synthase 1, PSS-1, PtdSer synthase 1, Serine-exchange enzyme I

3 Images
Immunoprecipitation - Anti-PTDSS1 antibody [EPR8118(2)] - BSA and Azide free (AB249322)
  • IP

Unknown

Immunoprecipitation - Anti-PTDSS1 antibody [EPR8118(2)] - BSA and Azide free (AB249322)

This data was developed using ab157222, the same antibody clone in a different buffer formulation.

Immunoprecipitation : 293T cell lysate immunoprecipitated with ab157222 at 1/10

Western Blot : ab157222 at 1/1000 labeling PTDSS1 in immunoprecipitation pellet from 293T cell lysate

Secondary Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size : 56 kDa

The observed band size of ab157222 is 42kda, this is attributed to the high content of hydrophobic amino acids in PSS1 (PMID : 8898108 and PMID : 10938271).

All lanes:

Immunoprecipitation - Anti-PTDSS1 antibody [EPR8118(2)] (<a href='/en-us/products/primary-antibodies/ptdss1-antibody-epr81182-ab157222'>ab157222</a>)

Predicted band size: 56 kDa

false

Western blot - Anti-PTDSS1 antibody [EPR8118(2)] - BSA and Azide free (AB249322)
  • WB

Unknown

Western blot - Anti-PTDSS1 antibody [EPR8118(2)] - BSA and Azide free (AB249322)

This data was developed using ab157222, the same antibody clone in a different buffer formulation.

The observed band size of ab157222 is 42kda, this is attributed to the high content of hydrophobic amino acids in PSS1 (PMID : 8898108 and 10938271).

All lanes:

Western blot - Anti-PTDSS1 antibody [EPR8118(2)] (<a href='/en-us/products/primary-antibodies/ptdss1-antibody-epr81182-ab157222'>ab157222</a>) at 1/1000 dilution

Lane 1:

Human fetal liver tissue lysate at 10 µg

Lane 2:

RAW 264.7 cell lysate at 10 µg

Lane 3:

NIH/3T3 cell lysate at 10 µg

Lane 4:

Human fetal brain tissue lysate at 10 µg

Lane 5:

A549 cell lysate at 10 µg

Lane 6:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 56 kDa

false

Western blot - Anti-PTDSS1 antibody [EPR8118(2)] - BSA and Azide free (AB249322)
  • WB

Lab

Western blot - Anti-PTDSS1 antibody [EPR8118(2)] - BSA and Azide free (AB249322)

This data was developed using ab157222, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Observed band size : 17 kDa (Recombinant protein fragment).

All lanes:

Western blot - Anti-PTDSS1 antibody [EPR8118(2)] (<a href='/en-us/products/primary-antibodies/ptdss1-antibody-epr81182-ab157222'>ab157222</a>) at 1/1000 dilution

All lanes:

His-tagged human PTDSS1 (aa2-35-GGGGS-aa2-35-GGGGS-aa2-35) recombinant protein 15ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 56 kDa

false

Exposure time: 1s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8118(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

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Biochemicals

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Reactivity data

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Product details

ab249322 is the carrier-free version of ab157222.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PTDSS1 also known as phosphatidylserine synthase 1 catalyzes the conversion of phosphatidylcholine to phosphatidylserine. This enzyme has a molecular mass of approximately 55 kDa and predominantly resides in the endoplasmic reticulum. It plays a role in lipid metabolism and is expressed in various tissues but is particularly active in the brain and liver. The activity of PTDSS1 is essential for maintaining the balance between different phospholipids in cellular membranes.
Biological function summary

Phosphatidylserine synthase 1 contributes to the production of phosphatidylserine an important phospholipid found in cell membranes and involved in cell signaling and apoptosis. PTDSS1 is not part of any known complex but regulates phosphatidylserine levels that aid in cellular functions such as lipid signaling and membrane structure. This regulation is essential as disruptions can influence cell function and viability.

Pathways

PTDSS1 is involved in the phospholipid biosynthesis pathway which is important for maintaining the function of cellular membranes. It influences pathways associated with cell survival and apoptosis due to its role in balancing phospholipid composition. PTDSS1 interplays with other proteins in these pathways including kinases and phosphatases which modulate cell signaling and membrane dynamics.

PTDSS1 alterations have connections to conditions such as neurological disorders and metabolic syndromes. Disruption in phosphatidylserine levels can contribute to neurodegenerative diseases where PTDSS1 interacts with proteins involved in cell death and survival pathways. Additionally abnormalities in PTDSS1 expression or function might link to metabolic disorders affecting lipid metabolism and cellular energy balance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes a base-exchange reaction in which the polar head group of phosphatidylethanolamine (PE) or phosphatidylcholine (PC) is replaced by L-serine (PubMed : 19014349, PubMed : 24241535). Catalyzes mainly the conversion of phosphatidylcholine (PubMed : 19014349, PubMed : 24241535). Also converts, in vitro and to a lesser extent, phosphatidylethanolamine (PubMed : 19014349, PubMed : 24241535).
See full target information PTDSS1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com