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AB137337

Anti-PTEN antibody

4

(1 Review)

|

(8 Publications)

Rabbit Polyclonal PTEN antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Rat, Mouse, Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PTEN aa 150-400.

View Alternative Names

MMAC1, TEP1, PTEN, Inositol polyphosphate 3-phosphatase, Mutated in multiple advanced cancers 1, Phosphatase and tensin homolog

9 Images
Immunocytochemistry/ Immunofluorescence - Anti-PTEN antibody (AB137337)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PTEN antibody (AB137337)

Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labelling PTEN with ab137337 (Green) at 1/500 dilution. Alpha-tubulin filaments were labeled red.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody (AB137337)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody (AB137337)

Paraffin-embedded human ovarian cancer tissue stained for PTEN protein at cytoplasm with ab137337 at a 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody (AB137337)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody (AB137337)

Paraffin-embedded human normal colon tissue stained for PTEN protein at cytoplasm with ab137337 at a 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, 20 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody (AB137337)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody (AB137337)

Paraffin-embedded rat middle brain tissue stained for PTEN protein at cytosol and nucleus with ab137337 at a 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : (EDTA based, pH 8.0) buffer, 15min.

Western blot - Anti-PTEN antibody (AB137337)
  • WB

Supplier Data

Western blot - Anti-PTEN antibody (AB137337)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-PTEN antibody (ab137337) at 1/500 dilution

All lanes:

293T Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 47 kDa

false

Western blot - Anti-PTEN antibody (AB137337)
  • WB

Supplier Data

Western blot - Anti-PTEN antibody (AB137337)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-PTEN antibody (ab137337) at 1/500 dilution

All lanes:

HL-60 Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 47 kDa

false

Western blot - Anti-PTEN antibody (AB137337)
  • WB

Supplier Data

Western blot - Anti-PTEN antibody (AB137337)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-PTEN antibody (ab137337) at 1/500 dilution

All lanes:

PC12 Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 47 kDa

false

Western blot - Anti-PTEN antibody (AB137337)
  • WB

Unknown

Western blot - Anti-PTEN antibody (AB137337)

10% SDS PAGE

All lanes:

Western blot - Anti-PTEN antibody (ab137337) at 1/1000 dilution

All lanes:

NIH 3T3 whole cell lysate at 30 µg

Predicted band size: 47 kDa,50 kDa

false

Western blot - Anti-PTEN antibody (AB137337)
  • WB

CiteAb

Western blot - Anti-PTEN antibody (AB137337)

PTEN western blot using anti-PTEN antibody ab137337. Publication image and figure legend from Zuluaga Tamayo, M., Barzegari, A., et al., 2017, Oxid Med Cell Longev, PubMed 29090040.

ab137337 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab137337 please see the product overview.

The molecular protection mechanism of CD-A complex against ROS. CD-A complex activates (a, b) Nrf2/HO-1/NQO1 and (c, d) PTEN/PI3K/AKT endogenous pathways. (e) Schematic representation of the molecular pathway of CD-A protective action on endothelial cells under oxidative stress. Levels not connected by the same letter are significantly different (p < 0.01).

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human PTEN aa 150-400. The exact immunogen used to generate this antibody is proprietary information.

P60484

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PTEN protein also known as phosphatase and tensin homolog is a phosphatase enzyme with a molecular mass of approximately 47 kDa. It acts mechanically by removing phosphate groups from phosphatidylinositol (345)-trisphosphate (PIP3) converting it to phosphatidylinositol (45)-bisphosphate. PTEN is ubiquitously expressed in various tissues with pronounced presence in the brain lung kidney and testis. This enzyme is an important regulator of cellular functions through its impact on signaling pathways.
Biological function summary

PTEN plays important roles in cellular processes like apoptosis cell proliferation and migration. It negatively regulates the PI3K/AKT signaling pathway critical for cell survival and growth. PTEN is not part of a larger protein complex but interacts with various other proteins modulating its activity. It maintains cellular homeostasis by balancing growth-promoting signals with cell cycle arrest and apoptotic pathways.

Pathways

PTEN is an important component in the PI3K/AKT and mTOR signaling pathways. These pathways regulate cell growth metabolism and survival and are interlinked with insulin signaling and cancer progression. PTEN's function directly interacts with proteins such as AKT and mTOR serving as a checkpoint that ensures controlled cellular proliferation. This positions PTEN as a tumor suppressor inhibiting uncontrolled cell growth via modulation of these pathways.

PTEN mutations or deletions are strongly associated with various types of cancers including breast and prostate cancer. PTEN interaction with the PI3K/AKT pathway influences cancer development often through loss-of-function mutations leading to unrestrained cellular growth. Beyond cancer PTEN mutations also relate to neurological disorders like Autism Spectrum Disorder where it affects signaling pathways involving proteins like mTOR. Understanding PTEN's role aids in unravelling the mechanistic underpinnings of these diseases paving the way for targeted therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins (PubMed : 9187108, PubMed : 9256433, PubMed : 9616126). Also functions as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring of PtdIns(3,4,5)P3/phosphatidylinositol 3,4,5-trisphosphate, PtdIns(3,4)P2/phosphatidylinositol 3,4-diphosphate and PtdIns3P/phosphatidylinositol 3-phosphate with a preference for PtdIns(3,4,5)P3 (PubMed : 16824732, PubMed : 26504226, PubMed : 9593664, PubMed : 9811831). Furthermore, this enzyme can also act as a cytosolic inositol 3-phosphatase acting on Ins(1,3,4,5,6)P5/inositol 1,3,4,5,6 pentakisphosphate and possibly Ins(1,3,4,5)P4/1D-myo-inositol 1,3,4,5-tetrakisphosphate (PubMed : 11418101, PubMed : 15979280). Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival (PubMed : 31492966, PubMed : 37279284). The unphosphorylated form cooperates with MAGI2 to suppress AKT1 activation (PubMed : 11707428). In motile cells, suppresses the formation of lateral pseudopods and thereby promotes cell polarization and directed movement (PubMed : 22279049). Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation (PubMed : 22279049). Required for growth factor-induced epithelial cell migration; growth factor stimulation induces PTEN phosphorylation which changes its binding preference from the p85 regulatory subunit of the PI3K kinase complex to DLC1 and results in translocation of the PTEN-DLC1 complex to the posterior of migrating cells to promote RHOA activation (PubMed : 26166433). Meanwhile, TNS3 switches binding preference from DLC1 to p85 and the TNS3-p85 complex translocates to the leading edge of migrating cells to activate RAC1 activation (PubMed : 26166433). Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation (By similarity). Involved in the regulation of synaptic function in excitatory hippocampal synapses. Recruited to the postsynaptic membrane upon NMDA receptor activation, is required for the modulation of synaptic activity during plasticity. Enhancement of lipid phosphatase activity is able to drive depression of AMPA receptor-mediated synaptic responses, activity required for NMDA receptor-dependent long-term depression (LTD) (By similarity). May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue. The nuclear monoubiquitinated form possesses greater apoptotic potential, whereas the cytoplasmic nonubiquitinated form induces less tumor suppressive ability (PubMed : 10468583, PubMed : 18716620).. Isoform alpha. Functional kinase, like isoform 1 it antagonizes the PI3K-AKT/PKB signaling pathway. Plays a role in mitochondrial energetic metabolism by promoting COX activity and ATP production, via collaboration with isoform 1 in increasing protein levels of PINK1.
See full target information PTEN
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

CNS neuroscience & therapeutics 30:e70033 PubMed39267282

2024

Transcranial direct current stimulation enhances the protective effect of isoflurane preconditioning on cerebral ischemia/reperfusion injury: A new mechanism associated with the nuclear protein Akirin2.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangyi Kong,Wenyuan Lyu,Xiaojie Lin,Hao Feng,Lin Xu,Chengwei Li,Xinyi Sun,Chunlong Lin,Jianjun Li,Penghui Wei

Experimental and therapeutic medicine 26:463 PubMed37664676

2023

Effects of DJ‑1 on apoptosis and mitophagy of glomerular podocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Xiao,Junjie Tan,Li Yu,Guosheng Liu,Shengyou Yu

Genetics and molecular biology 45:e20220023 PubMed35993557

2022

Circular RNA circENTPD7 suppresses the accumulation of PTEN to promote cell proliferation in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hongwei Yu,Yibin Zhang,Lu Zhang,Rufei Yang,Zhiwei Liao,Tongchong Zhou

Cell & bioscience 12:98 PubMed35765066

2022

DNA methyltransferase 1 knockdown reverses PTEN and VDR by mediating demethylation of promoter and protects against renal injuries in hepatitis B virus-associated glomerulonephritis.

Applications

Unspecified application

Species

Unspecified reactive species

Haochen Guan,Nan Zhu,Gang Tang,Yi Du,Ling Wang,Weijie Yuan

Scientific reports 12:2776 PubMed35177768

2022

Human adipose mesenchymal stem cells modulate inflammation and angiogenesis through exosomes.

Applications

Unspecified application

Species

Unspecified reactive species

June Seok Heo,Sinyoung Kim

Cancer biology & therapy 22:465-477 PubMed34486497

2021

Transcription factor KLF2 enhances the sensitivity of breast cancer cells to cisplatin by suppressing kinase WEE1.

Applications

Unspecified application

Species

Unspecified reactive species

Ruiqing Li,Jiejing Chen,Xiaokang Gao,Guoqin Jiang

Cancer biology & therapy 21:452-462 PubMed32089062

2020

Knockdown of lncRNA CCAT1 enhances sensitivity of paclitaxel in prostate cancer via regulating miR-24-3p and FSCN1.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohui Li,Xingtao Han,Pengtao Wei,Jinhui Yang,Jiantao Sun

Oxidative medicine and cellular longevity 2017:8073798 PubMed29090040

2017

Oxidative Stress Regulation on Endothelial Cells by Hydrophilic Astaxanthin Complex: Chemical, Biological, and Molecular Antioxidant Activity Evaluation.

Applications

Unspecified application

Species

Unspecified reactive species

M Zuluaga,A Barzegari,D Letourneur,V Gueguen,G Pavon-Djavid
View all publications
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