Anti-PTEN antibody [EPR9941-2]

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-PTEN antibody [EPR9941-2] (AB170941)

Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling PTEN with purified ab170941 at 1/120 dilution (10ug/ml) (red). Cells were fixed with 80% methanol and permeabilised with 0.1% Tween-20. A Goat anti rabbit IgG (Alexa Fluor^® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) (ab172730) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [EPR9941-2] (AB170941)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human pancreas tissue sections labeling PTEN with Purified ab170941 at 1 : 50 dilution (5.3 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control : PBS instead of the primary antibody.Hematoxylinwas used as a counterstain.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [EPR9941-2] (AB170941)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling PTEN with unpurifed ab170941 at 1/50 dilution.

Heat mediated antigen retrieval was performed using citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [EPR9941-2] (AB170941)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat cerebrum tissue sections labeling PTEN with Purified ab170941 at 1 : 50 dilution (5.3 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control : PBS instead of the primary antibody.Hematoxylinwas used as a counterstain

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [EPR9941-2] (AB170941)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebrum tissue sections labeling PTEN with Purified ab170941 at 1 : 50 dilution (5.3 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control : PBS instead of the primary antibody.Hematoxylinwas used as a counterstain

• WB

Lab

Western blot - Anti-PTEN antibody [EPR9941-2] (AB170941)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : PTEN knockout HAP1 cell lysate (20 μg)
Lanes 1 and 2 : Merged signal (red and green). Green - ab170941 observed at 47 kDa. Red - loading control, ab8245, observed at 37 kDa.

Unpurified ab170941 was shown to specifically react with PTEN in wild-type HAP1 cells. No band was observed when PTEN knockout samples were used. Wild-type and PTEN knockout samples were subjected to SDS-PAGE, ab170941 and ab8245 (loading control to GAPDH) were diluted 1/500 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1hr at room temperature before imaging.

All lanes:

Western blot - Anti-PTEN antibody [EPR9941-2] (ab170941)

Predicted band size: 47 kDa

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• WB

Unknown

Western blot - Anti-PTEN antibody [EPR9941-2] (AB170941)

Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PTEN antibody [EPR9941-2] (ab170941) at 0.05 µg/mL

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) whole cell lysates at 20 µg

Lane 3:

Rat brain lysates at 20 µg

Lane 4:

Mouse brain lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 47 kDa

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• WB

Supplier Data

Western blot - Anti-PTEN antibody [EPR9941-2] (AB170941)

All lanes:

Western blot - Anti-PTEN antibody [EPR9941-2] (ab170941) at 1/1000 dilution

Lane 1:

HeLa cell lysates at 10 µg

Lane 2:

MCF7 cell lysates at 10 µg

Lane 3:

293T cell lysates at 10 µg

Predicted band size: 47 kDa

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• WB

CiteAb

Western blot - Anti-PTEN antibody [EPR9941-2] (AB170941)

PTEN western blot using anti-PTEN antibody [EPR9941-2] ab170941. Publication image and figure legend from Wu, D. & Wang, C., 2020, Front Neurol, PubMed 32411077.

ab170941 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab170941 please see the product overview.

Effect of miR-155 on the expression of PTEN, Caspase-3 and Caspase-9 in U87-MG cells. (A) Effect of miR-155 on the expression of PTEN, caspase-3 and caspase-9 mRNA in U87-MG cells. (B) Effect of miR-155 on PTEN, caspase-3 and caspase-9 protein expression in U87-MG cells. (C) Gray statistical value of WB experiment; *p < 0.05 vs. control group, **p < 0.01 vs. control group.

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