Anti-PTEN antibody [EPR9941-2]

9 product images

• 

  Western blot - Anti-PTEN antibody [EPR9941-2] (ab170941)

  Blocking and diluting buffer: 5% NFDM/TBST.

  All lanes: Western blot - Anti-PTEN antibody [EPR9941-2] (ab170941) at 0.05 µg/mL

  Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

  Lane 2: 293T (Human embryonic kidney epithelial cell) whole cell lysates at 20 µg

  Lane 3: Rat brain lysates at 20 µg

  Lane 4: Mouse brain lysates at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Predicted band size: 47 kDa

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [EPR9941-2] (ab170941)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat cerebrum tissue sections labeling PTEN with Purified ab170941 at 1:50 dilution (5.3 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [EPR9941-2] (ab170941)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebrum tissue sections labeling PTEN with Purified ab170941 at 1:50 dilution (5.3 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [EPR9941-2] (ab170941)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human pancreas tissue sections labeling PTEN with Purified ab170941 at 1:50 dilution (5.3 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain.

• 

  Western blot - Anti-PTEN antibody [EPR9941-2] (ab170941)

  Lane 1: Wild-type HAP1 cell lysate (20 μg)
  Lane 2: PTEN knockout HAP1 cell lysate (20 μg)
  Lanes 1 and 2: Merged signal (red and green). Green - ab170941 observed at 47 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
  

  
  Unpurified ab170941 was shown to specifically react with PTEN in wild-type HAP1 cells. No band was observed when PTEN knockout samples were used. Wild-type and PTEN knockout samples were subjected to SDS-PAGE, ab170941 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted 1/500 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10,000 dilution for 1hr at room temperature before imaging.

  All lanes: Western blot - Anti-PTEN antibody [EPR9941-2] (ab170941)

  Predicted band size: 47 kDa

• 

  Flow Cytometry (Intracellular) - Anti-PTEN antibody [EPR9941-2] (ab170941)

  Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling PTEN with purified ab170941 at 1/120 dilution (10ug/ml) (red). Cells were fixed with 80% methanol and permeabilised with 0.1% Tween-20. A Goat anti rabbit IgG (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
  

• 

  Western blot - Anti-PTEN antibody [EPR9941-2] (ab170941)

  All lanes: Western blot - Anti-PTEN antibody [EPR9941-2] (ab170941) at 1/1000 dilution

  Lane 1: HeLa cell lysates at 10 µg

  Lane 2: MCF7 cell lysates at 10 µg

  Lane 3: 293T cell lysates at 10 µg

  Predicted band size: 47 kDa

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [EPR9941-2] (ab170941)

  Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling PTEN with unpurifed ab170941 at 1/50 dilution.

  Heat mediated antigen retrieval was performed using citrate buffer pH 6 before commencing with IHC staining protocol.

• 

  Image collected and cropped by CiteAb under a CC-BY license from the publication

  Western blot - Anti-PTEN antibody [EPR9941-2] (ab170941)

  PTEN western blot using anti-PTEN antibody [EPR9941-2] ab170941. Publication image and figure legend from Wu, D. & Wang, C., 2020, Front Neurol, PubMed 32411077.

  
  

  ab170941 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab170941 please see the product overview.

  Effect of miR-155 on the expression of PTEN, Caspase-3 and Caspase-9 in U87-MG cells. (A) Effect of miR-155 on the expression of PTEN, caspase-3 and caspase-9 mRNA in U87-MG cells. (B) Effect of miR-155 on PTEN, caspase-3 and caspase-9 protein expression in U87-MG cells. (C) Gray statistical value of WB experiment; *P < 0.05 vs. control group, **P < 0.01 vs. control group.