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Rabbit Recombinant Monoclonal PTEN antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 12 publications.


Images

Western blot - Anti-PTEN antibody [EPR9941] (AB154812), expandable thumbnail
  • Western blot - Anti-PTEN antibody [EPR9941] (AB154812), expandable thumbnail
  • Western blot - Anti-PTEN antibody [EPR9941] (AB154812), expandable thumbnail
  • Western blot - Anti-PTEN antibody [EPR9941] (AB154812), expandable thumbnail
  • Western blot - Anti-PTEN antibody [EPR9941] (AB154812), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Tested
Rat
Tested

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

For unpurified use at 1/40.

Species
Rat
Dilution info
1/1000 - 1/10000
Notes

For unpurified use at 1/40.

Species
Human
Dilution info
1/1000 - 1/10000
Notes

For unpurified use at 1/40.

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Target data

Function

Dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins (PubMed:9187108, PubMed:9256433, PubMed:9616126). Also functions as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring of PtdIns(3,4,5)P3/phosphatidylinositol 3,4,5-trisphosphate, PtdIns(3,4)P2/phosphatidylinositol 3,4-diphosphate and PtdIns3P/phosphatidylinositol 3-phosphate with a preference for PtdIns(3,4,5)P3 (PubMed:16824732, PubMed:26504226, PubMed:9593664, PubMed:9811831). Furthermore, this enzyme can also act as a cytosolic inositol 3-phosphatase acting on Ins(1,3,4,5,6)P5/inositol 1,3,4,5,6 pentakisphosphate and possibly Ins(1,3,4,5)P4/1D-myo-inositol 1,3,4,5-tetrakisphosphate (PubMed:11418101, PubMed:15979280). Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival (PubMed:31492966, PubMed:37279284). The unphosphorylated form cooperates with MAGI2 to suppress AKT1 activation (PubMed:11707428). In motile cells, suppresses the formation of lateral pseudopods and thereby promotes cell polarization and directed movement (PubMed:22279049). Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation (PubMed:22279049). Required for growth factor-induced epithelial cell migration; growth factor stimulation induces PTEN phosphorylation which changes its binding preference from the p85 regulatory subunit of the PI3K kinase complex to DLC1 and results in translocation of the PTEN-DLC1 complex to the posterior of migrating cells to promote RHOA activation (PubMed:26166433). Meanwhile, TNS3 switches binding preference from DLC1 to p85 and the TNS3-p85 complex translocates to the leading edge of migrating cells to activate RAC1 activation (PubMed:26166433). Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation (By similarity). Involved in the regulation of synaptic function in excitatory hippocampal synapses. Recruited to the postsynaptic membrane upon NMDA receptor activation, is required for the modulation of synaptic activity during plasticity. Enhancement of lipid phosphatase activity is able to drive depression of AMPA receptor-mediated synaptic responses, activity required for NMDA receptor-dependent long-term depression (LTD) (By similarity). May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue. The nuclear monoubiquitinated form possesses greater apoptotic potential, whereas the cytoplasmic nonubiquitinated form induces less tumor suppressive ability (PubMed:10468583, PubMed:18716620). Isoform alpha. Functional kinase, like isoform 1 it antagonizes the PI3K-AKT/PKB signaling pathway. Plays a role in mitochondrial energetic metabolism by promoting COX activity and ATP production, via collaboration with isoform 1 in increasing protein levels of PINK1.

Alternative names

MMAC1, TEP1, PTEN, Inositol polyphosphate 3-phosphatase, Mutated in multiple advanced cancers 1, Phosphatase and tensin homolog

Recommended products

Rabbit Recombinant Monoclonal PTEN antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 12 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR9941
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The PTEN protein also known as phosphatase and tensin homolog is a phosphatase enzyme with a molecular mass of approximately 47 kDa. It acts mechanically by removing phosphate groups from phosphatidylinositol (345)-trisphosphate (PIP3) converting it to phosphatidylinositol (45)-bisphosphate. PTEN is ubiquitously expressed in various tissues with pronounced presence in the brain lung kidney and testis. This enzyme is an important regulator of cellular functions through its impact on signaling pathways.

Biological function summary

PTEN plays important roles in cellular processes like apoptosis cell proliferation and migration. It negatively regulates the PI3K/AKT signaling pathway critical for cell survival and growth. PTEN is not part of a larger protein complex but interacts with various other proteins modulating its activity. It maintains cellular homeostasis by balancing growth-promoting signals with cell cycle arrest and apoptotic pathways.

Pathways

PTEN is an important component in the PI3K/AKT and mTOR signaling pathways. These pathways regulate cell growth metabolism and survival and are interlinked with insulin signaling and cancer progression. PTEN's function directly interacts with proteins such as AKT and mTOR serving as a checkpoint that ensures controlled cellular proliferation. This positions PTEN as a tumor suppressor inhibiting uncontrolled cell growth via modulation of these pathways.

Associated diseases and disorders

PTEN mutations or deletions are strongly associated with various types of cancers including breast and prostate cancer. PTEN interaction with the PI3K/AKT pathway influences cancer development often through loss-of-function mutations leading to unrestrained cellular growth. Beyond cancer PTEN mutations also relate to neurological disorders like Autism Spectrum Disorder where it affects signaling pathways involving proteins like mTOR. Understanding PTEN's role aids in unravelling the mechanistic underpinnings of these diseases paving the way for targeted therapeutic interventions.

Product promise

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6 product images

  • Western blot - Anti-PTEN antibody [EPR9941] (ab154812), expandable thumbnail

    Western blot - Anti-PTEN antibody [EPR9941] (ab154812)

    Lanes 1- 2: Merged signal (red and green). Green - ab154812 observed at 47 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.

    ab154812 was shown to react with PTEN in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line Human PTEN knockout HeLa cell line ab255419 (knockout cell lysate Human PTEN knockout HeLa cell lysate ab263829) was used. Wild-type HeLa and PTEN knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab154812 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-PTEN antibody [EPR9941] (ab154812) at 1/10000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: PTEN knockout HeLa cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 47 kDa

    Observed band size: 47 kDa

  • Western blot - Anti-PTEN antibody [EPR9941] (ab154812), expandable thumbnail

    Western blot - Anti-PTEN antibody [EPR9941] (ab154812)

    Lanes 1 and 2: Merged signal (red and green). Green - ab154812 observed at 51 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.


    ab154812 was shown to specifically recognize PTEN in wild-type HAP1 cells. No band was observed when PTEN knockout samples were used. Wild-type and PTEN knockout samples were subjected to SDS-PAGE, ab154812 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10,000 dilution for 1hr at room temperature before imaging.

    All lanes: Western blot - Anti-PTEN antibody [EPR9941] (ab154812) at 1/1000 dilution

    Lane 1: Wild-type HAP1 cell lysate at 20 µg

    Lane 2: PTEN knockout HAP1 cell lysate at 20 µg

    Predicted band size: 47 kDa

  • Western blot - Anti-PTEN antibody [EPR9941] (ab154812), expandable thumbnail

    Western blot - Anti-PTEN antibody [EPR9941] (ab154812)

    All lanes: Western blot - Anti-PTEN antibody [EPR9941] (ab154812) at 1/1000 dilution

    Lane 1: Mouse brain Lysate at 10 µg

    Lane 2: Rat brain Lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

    Predicted band size: 47 kDa

    Observed band size: 54 kDa

  • Western blot - Anti-PTEN antibody [EPR9941] (ab154812), expandable thumbnail

    Western blot - Anti-PTEN antibody [EPR9941] (ab154812)

    All lanes: Western blot - Anti-PTEN antibody [EPR9941] (ab154812) at 1/1000 dilution

    Lane 1: HeLa cell Lysate at 10 µg

    Lane 2: Neuro-2a cell Lysate at 10 µg

    Lane 3: Rat spleen cell Lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

    Predicted band size: 47 kDa

    Observed band size: 54 kDa

  • Western blot - Anti-PTEN antibody [EPR9941] (ab154812), expandable thumbnail

    Western blot - Anti-PTEN antibody [EPR9941] (ab154812)

    All lanes: Western blot - Anti-PTEN antibody [EPR9941] (ab154812) at 1/1000 dilution

    Lane 1: HeLa cell lysate at 10 µg

    Lane 2: MCF7 cell lysate at 10 µg

    Lane 3: 293T cell lysate at 10 µg

    Lane 4: A431 cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 47 kDa

  • Western blot - Anti-PTEN antibody [EPR9941] (ab154812), expandable thumbnail

    Image collected and cropped by CiteAb under a CC-BY license from the publication

    Western blot - Anti-PTEN antibody [EPR9941] (ab154812)

    PTEN western blot using anti-PTEN antibody [EPR9941] ab154812. Publication image and figure legend from Noll, A., Illuzzi, G., et al., 2016, Cancer Cell Int, PubMed 27375368.


    ab154812 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab154812 please see the product overview.

    PARG deficiency is not synthetic lethal with PTEN deficiency. a The PTEN-mutated MDA-MB-468 cell line is not sensitive to PARG depletion. Clonogenic survival (left panel) of MDA-MB-468 cells after siCTL and siPARG transfection. Results are depicted as box plots showing distribution of data from 5 individual experiments. Relative cell number 72 h post-siRNA transfection (right panel) is counted from 5 individual experiments. PARG depletion was verified by western blot (middle panel). b Clonogenic survival (left panel) of MDA-MB-231 cells after single or combined siRNA-mediated depletion of BRCA1, PARG and PTEN. Results are depicted as box plots showing distribution of data from 5 independent experiments. Percentage of viable cells relative to siCTL-transfected cells 72 h post-transfection (right panel) is counted from 5 individual experiments. BRCA1, PARG and PTEN siRNA-mediated depletions compared to untransfected or siCTL transfected cells were verified by western blot (middle panel). The arrow points to BRCA1 signal above a non-specific band (#). p: 0.01 < * < 0.05; 0.05 < ns, not significant

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