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AB154812

Anti-PTEN antibody [EPR9941]

  • 20ul selling size
  • RabMAb
  • Recombinant
  • KO Validated
  • What is this?

4

(1 Review)

|

(13 Publications)

Rabbit Recombinant Monoclonal PTEN antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 13 publications.

View Alternative Names

MMAC1, TEP1, PTEN, Inositol polyphosphate 3-phosphatase, Mutated in multiple advanced cancers 1, Phosphatase and tensin homolog

6 Images
Western blot - Anti-PTEN antibody [EPR9941] (AB154812)
  • WB

Unknown

Western blot - Anti-PTEN antibody [EPR9941] (AB154812)

All lanes:

Western blot - Anti-PTEN antibody [EPR9941] (ab154812) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

MCF7 cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Lane 4:

A431 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 47 kDa

false

Western blot - Anti-PTEN antibody [EPR9941] (AB154812)
  • WB

Lab

Western blot - Anti-PTEN antibody [EPR9941] (AB154812)

Lanes 1 and 2 : Merged signal (red and green). Green - ab154812 observed at 51 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab154812 was shown to specifically recognize PTEN in wild-type HAP1 cells. No band was observed when PTEN knockout samples were used. Wild-type and PTEN knockout samples were subjected to SDS-PAGE, ab154812 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1hr at room temperature before imaging.

All lanes:

Western blot - Anti-PTEN antibody [EPR9941] (ab154812) at 1/1000 dilution

Lane 1:

Wild-type HAP1 cell lysate at 20 µg

Lane 2:

PTEN knockout HAP1 cell lysate at 20 µg

Predicted band size: 47 kDa

false

Western blot - Anti-PTEN antibody [EPR9941] (AB154812)
  • WB

Lab

Western blot - Anti-PTEN antibody [EPR9941] (AB154812)

Lanes 1- 2 : Merged signal (red and green). Green - ab154812 observed at 47 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab154812 was shown to react with PTEN in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab255419 (knockout cell lysate ab263829) was used. Wild-type HeLa and PTEN knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab154812 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PTEN antibody [EPR9941] (ab154812) at 1/10000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

PTEN knockout HeLa cell lysate at 20 µg

Predicted band size: 47 kDa

Observed band size: 47 kDa

false

Western blot - Anti-PTEN antibody [EPR9941] (AB154812)
  • WB

Supplier Data

Western blot - Anti-PTEN antibody [EPR9941] (AB154812)

All lanes:

Western blot - Anti-PTEN antibody [EPR9941] (ab154812) at 1/1000 dilution

Lane 1:

Mouse brain Lysate at 10 µg

Lane 2:

Rat brain Lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

Predicted band size: 47 kDa

Observed band size: 54 kDa

false

Western blot - Anti-PTEN antibody [EPR9941] (AB154812)
  • WB

Supplier Data

Western blot - Anti-PTEN antibody [EPR9941] (AB154812)

All lanes:

Western blot - Anti-PTEN antibody [EPR9941] (ab154812) at 1/1000 dilution

Lane 1:

HeLa cell Lysate at 10 µg

Lane 2:

Neuro-2a cell Lysate at 10 µg

Lane 3:

Rat spleen cell Lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

Predicted band size: 47 kDa

Observed band size: 54 kDa

false

Western blot - Anti-PTEN antibody [EPR9941] (AB154812)
  • WB

CiteAb

Western blot - Anti-PTEN antibody [EPR9941] (AB154812)

PTEN western blot using anti-PTEN antibody [EPR9941] ab154812. Publication image and figure legend from Noll, A., Illuzzi, G., et al., 2016, Cancer Cell Int, PubMed 27375368.

ab154812 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab154812 please see the product overview.

PARG deficiency is not synthetic lethal with PTEN deficiency. a The PTEN-mutated MDA-MB-468 cell line is not sensitive to PARG depletion. Clonogenic survival (left panel) of MDA-MB-468 cells after siCTL and siPARG transfection. Results are depicted as box plots showing distribution of data from 5 individual experiments. Relative cell number 72 h post-siRNA transfection (right panel) is counted from 5 individual experiments. PARG depletion was verified by western blot (middle panel). b Clonogenic survival (left panel) of MDA-MB-231 cells after single or combined siRNA-mediated depletion of BRCA1, PARG and PTEN. Results are depicted as box plots showing distribution of data from 5 independent experiments. Percentage of viable cells relative to siCTL-transfected cells 72 h post-transfection (right panel) is counted from 5 individual experiments. BRCA1, PARG and PTEN siRNA-mediated depletions compared to untransfected or siCTL transfected cells were verified by western blot (middle panel). The arrow points to BRCA1 signal above a non-specific band (#). p : 0.01 < * < 0.05; 0.05 < ns, not significant

false

  • Carrier free

    Anti-PTEN antibody [EPR9941] - BSA and Azide free

  • Carrier free

    Anti-PTEN antibody [EPR9941] - BSA and Azide free (Capture)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9941

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PTEN protein also known as phosphatase and tensin homolog is a phosphatase enzyme with a molecular mass of approximately 47 kDa. It acts mechanically by removing phosphate groups from phosphatidylinositol (345)-trisphosphate (PIP3) converting it to phosphatidylinositol (45)-bisphosphate. PTEN is ubiquitously expressed in various tissues with pronounced presence in the brain lung kidney and testis. This enzyme is an important regulator of cellular functions through its impact on signaling pathways.
Biological function summary

PTEN plays important roles in cellular processes like apoptosis cell proliferation and migration. It negatively regulates the PI3K/AKT signaling pathway critical for cell survival and growth. PTEN is not part of a larger protein complex but interacts with various other proteins modulating its activity. It maintains cellular homeostasis by balancing growth-promoting signals with cell cycle arrest and apoptotic pathways.

Pathways

PTEN is an important component in the PI3K/AKT and mTOR signaling pathways. These pathways regulate cell growth metabolism and survival and are interlinked with insulin signaling and cancer progression. PTEN's function directly interacts with proteins such as AKT and mTOR serving as a checkpoint that ensures controlled cellular proliferation. This positions PTEN as a tumor suppressor inhibiting uncontrolled cell growth via modulation of these pathways.

PTEN mutations or deletions are strongly associated with various types of cancers including breast and prostate cancer. PTEN interaction with the PI3K/AKT pathway influences cancer development often through loss-of-function mutations leading to unrestrained cellular growth. Beyond cancer PTEN mutations also relate to neurological disorders like Autism Spectrum Disorder where it affects signaling pathways involving proteins like mTOR. Understanding PTEN's role aids in unravelling the mechanistic underpinnings of these diseases paving the way for targeted therapeutic interventions.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins (PubMed : 9187108, PubMed : 9256433, PubMed : 9616126). Also functions as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring of PtdIns(3,4,5)P3/phosphatidylinositol 3,4,5-trisphosphate, PtdIns(3,4)P2/phosphatidylinositol 3,4-diphosphate and PtdIns3P/phosphatidylinositol 3-phosphate with a preference for PtdIns(3,4,5)P3 (PubMed : 16824732, PubMed : 26504226, PubMed : 9593664, PubMed : 9811831). Furthermore, this enzyme can also act as a cytosolic inositol 3-phosphatase acting on Ins(1,3,4,5,6)P5/inositol 1,3,4,5,6 pentakisphosphate and possibly Ins(1,3,4,5)P4/1D-myo-inositol 1,3,4,5-tetrakisphosphate (PubMed : 11418101, PubMed : 15979280). Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival (PubMed : 31492966, PubMed : 37279284). The unphosphorylated form cooperates with MAGI2 to suppress AKT1 activation (PubMed : 11707428). In motile cells, suppresses the formation of lateral pseudopods and thereby promotes cell polarization and directed movement (PubMed : 22279049). Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation (PubMed : 22279049). Required for growth factor-induced epithelial cell migration; growth factor stimulation induces PTEN phosphorylation which changes its binding preference from the p85 regulatory subunit of the PI3K kinase complex to DLC1 and results in translocation of the PTEN-DLC1 complex to the posterior of migrating cells to promote RHOA activation (PubMed : 26166433). Meanwhile, TNS3 switches binding preference from DLC1 to p85 and the TNS3-p85 complex translocates to the leading edge of migrating cells to activate RAC1 activation (PubMed : 26166433). Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation (By similarity). Involved in the regulation of synaptic function in excitatory hippocampal synapses. Recruited to the postsynaptic membrane upon NMDA receptor activation, is required for the modulation of synaptic activity during plasticity. Enhancement of lipid phosphatase activity is able to drive depression of AMPA receptor-mediated synaptic responses, activity required for NMDA receptor-dependent long-term depression (LTD) (By similarity). May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue. The nuclear monoubiquitinated form possesses greater apoptotic potential, whereas the cytoplasmic nonubiquitinated form induces less tumor suppressive ability (PubMed : 10468583, PubMed : 18716620).. Isoform alpha. Functional kinase, like isoform 1 it antagonizes the PI3K-AKT/PKB signaling pathway. Plays a role in mitochondrial energetic metabolism by promoting COX activity and ATP production, via collaboration with isoform 1 in increasing protein levels of PINK1.
See full target information PTEN

Publications (13)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37298523

2023

miRNA-132/212 Deficiency Disrupts Selective Corticosterone Modulation of Dorsal vs. Ventral Hippocampal Metaplasticity.

Applications

Unspecified application

Species

Unspecified reactive species

Shima Kouhnavardi,Maureen Cabatic,M Carmen Mañas-Padilla,Marife-Astrid Malabanan,Tarik Smani,Ana Cicvaric,Edison Alejandro Muñoz Aranzalez,Xaver Koenig,Ernst Urban,Gert Lubec,Estela Castilla-Ortega,Francisco J Monje

Cancers 14: PubMed36139555

2022

Single Shot vs. Cocktail: A Comparison of Mono- and Combinative Application of miRNA-Targeted Mesyl Oligonucleotides for Efficient Antitumor Therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Svetlana Gaponova,Olga Patutina,Aleksandra Sen'kova,Ekaterina Burakova,Innokenty Savin,Andrey Markov,Elena Shmendel,Mikhail Maslov,Dmitry Stetsenko,Valentin Vlassov,Marina Zenkova

Cell death discovery 7:212 PubMed34381025

2021

Human umbilical cord mesenchymal stem cells-derived extracellular vesicles facilitate the repair of spinal cord injury via the miR-29b-3p/PTEN/Akt/mTOR axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Xiao,Weiwei Li,Dingchao Rong,Zhenchao Xu,Zhen Zhang,Hongru Ye,Liqiong Xie,Yunqi Wu,Yilu Zhang,Xiyang Wang

Proceedings of the National Academy of Sciences of the United States of America 117:32370-32379 PubMed33288723

2020

Mesyl phosphoramidate backbone modified antisense oligonucleotides targeting miR-21 with enhanced in vivo therapeutic potency.

Applications

Unspecified application

Species

Unspecified reactive species

Olga A Patutina,Svetlana K Gaponova Miroshnichenko,Aleksandra V Sen'kova,Innokenty A Savin,Daniil V Gladkikh,Ekaterine A Burakova,Alesya A Fokina,Mikhail A Maslov,Elena V Shmendel',Mattew J A Wood,Valentin V Vlassov,Sidney Altman,Dmitry A Stetsenko,Marina A Zenkova

Asian Pacific journal of cancer prevention : APJCP 21:391-397 PubMed32102516

2020

MIR-107, MIR-223-3P and MIR-21-5P Reveals Potential Biomarkers in Penile Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jaqueline Diniz Pinho,Gyl Eanes Barros Silva,Antonio Augusto Lima Teixeira Júnior,Marta Regina de Castro Belfort,Juliana Melo Mendes,Isabela Wernerck da Cunha,Luciana Gonçalves Quintana,José de Ribamar Rodrigues Calixto,Leudivan Ribeiro Nogueira,Ronald Wagner Pereira Coelho,André Salim Khayat

Scientific reports 9:2384 PubMed30787346

2019

Oncosuppressor-Mutated Cells as a Liquid Biopsy Test for Cancer-Screening.

Applications

Unspecified application

Species

Unspecified reactive species

Mohamed Abdouh,Zu-Hua Gao,Vincenzo Arena,Manuel Arena,Miguel N Burnier,Goffredo Orazio Arena

Scientific reports 9:528 PubMed30679653

2019

A role for miR-132 in learned safety.

Applications

Unspecified application

Species

Unspecified reactive species

Marianne Ronovsky,Alice Zambon,Ana Cicvaric,Vincent Boehm,Bastian Hoesel,Bernhard A Moser,Jiaye Yang,Johannes A Schmid,Wulf E Haubensak,Francisco J Monje,Daniela D Pollak

Nature methods 15:909-912 PubMed30377371

2018

A high-throughput pipeline for validation of antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

Krzysztof Sikorski,Adi Mehta,Marit Inngjerdingen,Flourina Thakor,Simon Kling,Tomas Kalina,Tuula A Nyman,Maria Ekman Stensland,Wei Zhou,Gustavo A de Souza,Lars Holden,Jan Stuchly,Markus Templin,Fridtjof Lund-Johansen

Journal of cellular physiology 233:4688-4706 PubMed29115668

2018

miR-26a promoted endometrial epithelium cells (EECs) proliferation and induced stromal cells (ESCs) apoptosis via the PTEN-PI3K/AKT pathway in dairy goats.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Zhang,Xiaorui Liu,Junze Liu,Xingna Ma,Zhanqin Zhou,Yuxuan Song,Binyun Cao

American journal of physiology. Lung cellular and 313:L230-L239 PubMed28522564

2017

Decreased phosphatase PTEN amplifies PI3K signaling and enhances proinflammatory cytokine release in COPD.

Applications

Unspecified application

Species

Unspecified reactive species

Satoru Yanagisawa,Jonathan R Baker,Chaitanya Vuppusetty,Peter Fenwick,Louise E Donnelly,Kazuhiro Ito,Peter J Barnes
View all publications

Product promise

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