Anti-PTEN antibody [EPR9941]
- 20ul selling size
- RabMAb
- Recombinant
- KO Validated
- What is this?
4
(1 Review)
|
(13 Publications)
Rabbit Recombinant Monoclonal PTEN antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 13 publications.
View Alternative Names
MMAC1, TEP1, PTEN, Inositol polyphosphate 3-phosphatase, Mutated in multiple advanced cancers 1, Phosphatase and tensin homolog
- WB
Unknown
Western blot - Anti-PTEN antibody [EPR9941] (AB154812)
All lanes:
Western blot - Anti-PTEN antibody [EPR9941] (ab154812) at 1/1000 dilution
Lane 1:
HeLa cell lysate at 10 µg
Lane 2:
MCF7 cell lysate at 10 µg
Lane 3:
293T cell lysate at 10 µg
Lane 4:
A431 cell lysate at 10 µg
Secondary
All lanes:
Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 47 kDa
false
- WB
Lab
Western blot - Anti-PTEN antibody [EPR9941] (AB154812)
Lanes 1 and 2 : Merged signal (red and green). Green - ab154812 observed at 51 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab154812 was shown to specifically recognize PTEN in wild-type HAP1 cells. No band was observed when PTEN knockout samples were used. Wild-type and PTEN knockout samples were subjected to SDS-PAGE, ab154812 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1hr at room temperature before imaging.
All lanes:
Western blot - Anti-PTEN antibody [EPR9941] (ab154812) at 1/1000 dilution
Lane 1:
Wild-type HAP1 cell lysate at 20 µg
Lane 2:
PTEN knockout HAP1 cell lysate at 20 µg
Predicted band size: 47 kDa
false
- WB
Lab
Western blot - Anti-PTEN antibody [EPR9941] (AB154812)
Lanes 1- 2 : Merged signal (red and green). Green - ab154812 observed at 47 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab154812 was shown to react with PTEN in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab255419 (knockout cell lysate ab263829) was used. Wild-type HeLa and PTEN knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab154812 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-PTEN antibody [EPR9941] (ab154812) at 1/10000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
PTEN knockout HeLa cell lysate at 20 µg
Predicted band size: 47 kDa
Observed band size: 47 kDa
false
- WB
Supplier Data
Western blot - Anti-PTEN antibody [EPR9941] (AB154812)
All lanes:
Western blot - Anti-PTEN antibody [EPR9941] (ab154812) at 1/1000 dilution
Lane 1:
Mouse brain Lysate at 10 µg
Lane 2:
Rat brain Lysate at 10 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution
Predicted band size: 47 kDa
Observed band size: 54 kDa
false
- WB
Supplier Data
Western blot - Anti-PTEN antibody [EPR9941] (AB154812)
All lanes:
Western blot - Anti-PTEN antibody [EPR9941] (ab154812) at 1/1000 dilution
Lane 1:
HeLa cell Lysate at 10 µg
Lane 2:
Neuro-2a cell Lysate at 10 µg
Lane 3:
Rat spleen cell Lysate at 10 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution
Predicted band size: 47 kDa
Observed band size: 54 kDa
false
- WB
CiteAb
Western blot - Anti-PTEN antibody [EPR9941] (AB154812)
PTEN western blot using anti-PTEN antibody [EPR9941] ab154812. Publication image and figure legend from Noll, A., Illuzzi, G., et al., 2016, Cancer Cell Int, PubMed 27375368.
ab154812 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab154812 please see the product overview.
PARG deficiency is not synthetic lethal with PTEN deficiency. a The PTEN-mutated MDA-MB-468 cell line is not sensitive to PARG depletion. Clonogenic survival (left panel) of MDA-MB-468 cells after siCTL and siPARG transfection. Results are depicted as box plots showing distribution of data from 5 individual experiments. Relative cell number 72 h post-siRNA transfection (right panel) is counted from 5 individual experiments. PARG depletion was verified by western blot (middle panel). b Clonogenic survival (left panel) of MDA-MB-231 cells after single or combined siRNA-mediated depletion of BRCA1, PARG and PTEN. Results are depicted as box plots showing distribution of data from 5 independent experiments. Percentage of viable cells relative to siCTL-transfected cells 72 h post-transfection (right panel) is counted from 5 individual experiments. BRCA1, PARG and PTEN siRNA-mediated depletions compared to untransfected or siCTL transfected cells were verified by western blot (middle panel). The arrow points to BRCA1 signal above a non-specific band (#). p : 0.01 < * < 0.05; 0.05 < ns, not significant
false
Related conjugates and formulations (2)
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Anti-PTEN antibody [EPR9941] - BSA and Azide free
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Anti-PTEN antibody [EPR9941] - BSA and Azide free (Capture)
Reactivity data
Product details
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PTEN plays important roles in cellular processes like apoptosis cell proliferation and migration. It negatively regulates the PI3K/AKT signaling pathway critical for cell survival and growth. PTEN is not part of a larger protein complex but interacts with various other proteins modulating its activity. It maintains cellular homeostasis by balancing growth-promoting signals with cell cycle arrest and apoptotic pathways.
Pathways
PTEN is an important component in the PI3K/AKT and mTOR signaling pathways. These pathways regulate cell growth metabolism and survival and are interlinked with insulin signaling and cancer progression. PTEN's function directly interacts with proteins such as AKT and mTOR serving as a checkpoint that ensures controlled cellular proliferation. This positions PTEN as a tumor suppressor inhibiting uncontrolled cell growth via modulation of these pathways.
Product protocols
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Target data
Publications (13)
Recent publications for all applications. Explore the full list and refine your search
International journal of molecular sciences 24: PubMed37298523
2023
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Cancers 14: PubMed36139555
2022
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Cell death discovery 7:212 PubMed34381025
2021
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Proceedings of the National Academy of Sciences of the United States of America 117:32370-32379 PubMed33288723
2020
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Asian Pacific journal of cancer prevention : APJCP 21:391-397 PubMed32102516
2020
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Scientific reports 9:2384 PubMed30787346
2019
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Scientific reports 9:528 PubMed30679653
2019
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Nature methods 15:909-912 PubMed30377371
2018
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Journal of cellular physiology 233:4688-4706 PubMed29115668
2018
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American journal of physiology. Lung cellular and 313:L230-L239 PubMed28522564
2017
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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