Name: Anti-PTEN antibody [Y184]
SKU: ab32199
Rating: 5 (15 reviews)

Anti-PTEN antibody [Y184] (ab32199) is a rabbit monoclonal antibody that is used to detect PTEN in Western Blot. Suitable for Human, Mouse samples.

- Specificity confirmed with PTEN knockout cell line validation

Images

Western blot - Anti-PTEN antibody [Y184] (AB32199), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product promiseTestedExpectedPredictedNot recommended

	WB
Human	Tested
Mouse	Tested
Rat	Predicted

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/10000	Notes For unpurified, use 1/500.
Species Human	Dilution info 1/10000	Notes For unpurified, use 1/500.

Predicted
Predicted

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes -

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Target data

See full target information PTEN

Function

Dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins (PubMed:9187108, PubMed:9256433, PubMed:9616126). Also functions as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring of PtdIns(3,4,5)P3/phosphatidylinositol 3,4,5-trisphosphate, PtdIns(3,4)P2/phosphatidylinositol 3,4-diphosphate and PtdIns3P/phosphatidylinositol 3-phosphate with a preference for PtdIns(3,4,5)P3 (PubMed:16824732, PubMed:26504226, PubMed:9593664, PubMed:9811831). Furthermore, this enzyme can also act as a cytosolic inositol 3-phosphatase acting on Ins(1,3,4,5,6)P5/inositol 1,3,4,5,6 pentakisphosphate and possibly Ins(1,3,4,5)P4/1D-myo-inositol 1,3,4,5-tetrakisphosphate (PubMed:11418101, PubMed:15979280). Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival (PubMed:31492966, PubMed:37279284). The unphosphorylated form cooperates with MAGI2 to suppress AKT1 activation (PubMed:11707428). In motile cells, suppresses the formation of lateral pseudopods and thereby promotes cell polarization and directed movement (PubMed:22279049). Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation (PubMed:22279049). Required for growth factor-induced epithelial cell migration; growth factor stimulation induces PTEN phosphorylation which changes its binding preference from the p85 regulatory subunit of the PI3K kinase complex to DLC1 and results in translocation of the PTEN-DLC1 complex to the posterior of migrating cells to promote RHOA activation (PubMed:26166433). Meanwhile, TNS3 switches binding preference from DLC1 to p85 and the TNS3-p85 complex translocates to the leading edge of migrating cells to activate RAC1 activation (PubMed:26166433). Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation (By similarity). Involved in the regulation of synaptic function in excitatory hippocampal synapses. Recruited to the postsynaptic membrane upon NMDA receptor activation, is required for the modulation of synaptic activity during plasticity. Enhancement of lipid phosphatase activity is able to drive depression of AMPA receptor-mediated synaptic responses, activity required for NMDA receptor-dependent long-term depression (LTD) (By similarity). May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue. The nuclear monoubiquitinated form possesses greater apoptotic potential, whereas the cytoplasmic nonubiquitinated form induces less tumor suppressive ability (PubMed:10468583, PubMed:18716620). Isoform alpha. Functional kinase, like isoform 1 it antagonizes the PI3K-AKT/PKB signaling pathway. Plays a role in mitochondrial energetic metabolism by promoting COX activity and ATP production, via collaboration with isoform 1 in increasing protein levels of PINK1.

Alternative names

MMAC1, TEP1, PTEN, Inositol polyphosphate 3-phosphatase, Mutated in multiple advanced cancers 1, Phosphatase and tensin homolog

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: Y184
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

Western blot - Anti-PTEN antibody [Y184] (ab32199)
Lanes 1 and 5: PTEN knockout HAP1 cell lysate (20 μg)
Lanes 2 and 6: Wild-type HAP1 cell lysate (20 μg)
Lane 2: Green signal from target – ab32199 observed at 47 kDa
Lanes 3 and 4: Red signal from loading control – Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa
Lanes 5 and 6: Merged (red and green) signal
ab32199 was shown to specifically recognize PTEN in wild-type HAP1 cells along with additional cross reactive bands. No band was observed when PTEN knockout samples were used. Wild-type and PTEN knockout samples were subjected to SDS-PAGE, ab32199 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted to 1/500 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging.
All lanes: Western blot - Anti-PTEN antibody [Y184] (ab32199)
Predicted band size: 47 kDa
Western blot - Anti-PTEN antibody [Y184] (ab32199)
Lanes 1: PTEN knockout HAP1 cell lysate (20 μg)
Lanes 2: Wild-type HAP1 cell lysate (20 μg)
Green signal from target
Red signal from loading control – Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa
This western blot image is a comparison between ab32199 and a competitor's top cited mouse monoclonal antibody.
All lanes: Western blot - Anti-PTEN antibody [Y184] (ab32199)
Predicted band size: 47 kDa
Western blot - Anti-PTEN antibody [Y184] (ab32199)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-PTEN antibody [Y184] (ab32199) at 1/10000 dilution
All lanes: Brain lysate at 10 µg
Secondary
All lanes: Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 47 kDa
Observed band size: 54 kDa
Western blot - Anti-PTEN antibody [Y184] (ab32199)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-PTEN antibody [Y184] (ab32199) at 1/10000 dilution
Lane 1: MCF7 whole cell lysate at 10 µg
Lane 2: HEK293 whole cell lysate at 10 µg
Secondary
All lanes: Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 47 kDa
Observed band size: 54 kDa
Western blot - Anti-PTEN antibody [Y184] (ab32199)
All lanes: Western blot - Anti-PTEN antibody [Y184] (ab32199) at 1/500 dilution
All lanes: MCF7 cell lysate
Predicted band size: 47 kDa
Observed band size: 54 kDa
This image is courtesy of an anonymous customer review
Western blot - Anti-PTEN antibody [Y184] (ab32199)
Treatment:
Lane 1 - control siRNA 24 hours
Lane 2 - PTEN siRNA 24 hours
Lane 3 - control siRNA 48 hours
Lane 4 - PTEN siRNA 48 hours
All lanes: Western blot - Anti-PTEN antibody [Y184] (ab32199) at 1/500 dilution
All lanes: Mouse primary bone marrow derived macrophage whole cell lysate at 50 µg
Secondary
All lanes: HRP-conjugated goat anti-rabbit IgG polyclonal
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 50 kDa
Exposure time: 30s
This image is courtesy of an anonymous customer review.
Western blot - Anti-PTEN antibody [Y184] (ab32199)
All lanes: Western blot - Anti-PTEN antibody [Y184] (ab32199) at 1/500 dilution
All lanes: RBL-2H3 whole cell lysate
Secondary
All lanes: Goat Anti-rabbit HRP at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 47 kDa

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Anti-PTEN antibody [Y184]

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