Rabbit Recombinant Monoclonal PTEN phospho T366 antibody. Suitable for IHC-P, Dot, WB and reacts with Human, Mouse, Rat, Synthetic peptide samples. Cited in 13 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN (phospho T366) antibody [EP229] (AB109454), expandable thumbnail](https://content.abcam.com/products/images/pten-phospho-t366-antibody-ep229-ab109454--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img139407.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN (phospho T366) antibody [EP229] (AB109454)")
![Western blot - Anti-PTEN (phospho T366) antibody [EP229] (AB109454), expandable thumbnail](https://content.abcam.com/products/images/pten-phospho-t366-antibody-ep229-ab109454--western-blot-img408368.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PTEN (phospho T366) antibody [EP229] (AB109454)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN (phospho T366) antibody [EP229] (AB109454), expandable thumbnail](https://content.abcam.com/products/images/pten-phospho-t366-antibody-ep229-ab109454--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408371.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN (phospho T366) antibody [EP229] (AB109454)")
![Dot Blot - Anti-PTEN (phospho T366) antibody [EP229] (AB109454), expandable thumbnail](https://content.abcam.com/products/images/pten-phospho-t366-antibody-ep229-ab109454--dot-blot-img408372.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-PTEN (phospho T366) antibody [EP229] (AB109454)")
![Western blot - Anti-PTEN (phospho T366) antibody [EP229] (AB109454), expandable thumbnail](https://content.abcam.com/products/images/pten-phospho-t366-antibody-ep229-ab109454--western-blot-img408370.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PTEN (phospho T366) antibody [EP229] (AB109454)")
Publications
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- Experimental and therapeutic medicine 23:522021Gut microbiota relieves inflammation in the substantia nigra of chronic Parkinson's disease by protecting the function of dopamine neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTian Zhang,Tan Wang,Xinxu Chen,Zhenqiang Zhao,Zhibin ChenPubMed 34934429
- Biology open 9:2020Methylated at promoter regions induces asthma occurrence via the PI3K/Akt/NFκB-mediated inflammation in IUGR mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Xing et. al.PubMed 32139393
- Molecular therapy. Nucleic acids 19:814-8262020The MSC-Derived Exosomal lncRNA H19 Promotes Wound Healing in Diabetic Foot Ulcers by Upregulating PTEN via MicroRNA-152-3p.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBo Li,Song Luan,Jing Chen,Yue Zhou,Tingting Wang,Zhijuan Li,Yili Fu,Aixia Zhai,Changlong BiPubMed 31958697
- BMC cancer 19:9282019Immunohistochemical validation of COL3A1, GPR158 and PITHD1 as prognostic biomarkers in early-stage ovarian carcinomas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHanna Engqvist et. al.PubMed 31533654
- PloS one 14:e02138312019Glycogen synthase kinase 3-β inhibition induces lymphangiogenesis through β-catenin-dependent and mTOR-independent pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBenjamin Stump et. al.PubMed 30964887
- Medical science monitor : international medical jo 24:8105-81142018Up-Regulation of Phosphatase in Regenerating Liver-3 (PRL-3) Contributes to Malignant Progression of Hepatocellular Carcinoma by Activating Phosphatase and Tensin Homolog Deleted on Chromosome Ten (PTEN)/Phosphoinositide 3-Kinase (PI3K)/AKT Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBing-Hui Li et. al.PubMed 30418964
- Oncology letters 16:984-9902018miR-222 promotes invasion and migration of ovarian carcinoma by targeting PTEN.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Gong et. al.PubMed 29963173
- Experimental and therapeutic medicine 15:2081-20872018MicroRNA-30a-3p overexpression improves sepsis-induced cell apoptosis and via the PTEN/PI3K/AKT signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuying Yang,Yongqiang Wang,Hongmei Gao,Bing WangPubMed 29434809
- Oncology letters 12:1139-11432016PREX2 promotes the proliferation, invasion and migration of pancreatic cancer cells by modulating the PI3K signaling pathway.
- Oncology letters 11:2223-22282016Upregulation of PREX2 promotes the proliferation and migration of hepatocellular carcinoma cells via PTEN-AKT signaling.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| IHC-P | Flow Cyt | Dot | WB | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Expected | Tested |
| Mouse | Expected | Not recommended | Expected | Tested |
| Rat | Expected | Not recommended | Expected | Tested |
| Synthetic peptide | Not recommended | Not recommended | Tested | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000 | Notes The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Synthetic peptide | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10000 | Notes - |
Species Mouse | Dilution info 1/10000 | Notes - |
Species Rat | Dilution info 1/10000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
Target data
Function
Dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins (PubMed:9187108, PubMed:9256433, PubMed:9616126). Also functions as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring of PtdIns(3,4,5)P3/phosphatidylinositol 3,4,5-trisphosphate, PtdIns(3,4)P2/phosphatidylinositol 3,4-diphosphate and PtdIns3P/phosphatidylinositol 3-phosphate with a preference for PtdIns(3,4,5)P3 (PubMed:16824732, PubMed:26504226, PubMed:9593664, PubMed:9811831). Furthermore, this enzyme can also act as a cytosolic inositol 3-phosphatase acting on Ins(1,3,4,5,6)P5/inositol 1,3,4,5,6 pentakisphosphate and possibly Ins(1,3,4,5)P4/1D-myo-inositol 1,3,4,5-tetrakisphosphate (PubMed:11418101, PubMed:15979280). Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival (PubMed:31492966, PubMed:37279284). The unphosphorylated form cooperates with MAGI2 to suppress AKT1 activation (PubMed:11707428). In motile cells, suppresses the formation of lateral pseudopods and thereby promotes cell polarization and directed movement (PubMed:22279049). Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation (PubMed:22279049). Required for growth factor-induced epithelial cell migration; growth factor stimulation induces PTEN phosphorylation which changes its binding preference from the p85 regulatory subunit of the PI3K kinase complex to DLC1 and results in translocation of the PTEN-DLC1 complex to the posterior of migrating cells to promote RHOA activation (PubMed:26166433). Meanwhile, TNS3 switches binding preference from DLC1 to p85 and the TNS3-p85 complex translocates to the leading edge of migrating cells to activate RAC1 activation (PubMed:26166433). Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation (By similarity). Involved in the regulation of synaptic function in excitatory hippocampal synapses. Recruited to the postsynaptic membrane upon NMDA receptor activation, is required for the modulation of synaptic activity during plasticity. Enhancement of lipid phosphatase activity is able to drive depression of AMPA receptor-mediated synaptic responses, activity required for NMDA receptor-dependent long-term depression (LTD) (By similarity). May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue. The nuclear monoubiquitinated form possesses greater apoptotic potential, whereas the cytoplasmic nonubiquitinated form induces less tumor suppressive ability (PubMed:10468583, PubMed:18716620). Isoform alpha. Functional kinase, like isoform 1 it antagonizes the PI3K-AKT/PKB signaling pathway. Plays a role in mitochondrial energetic metabolism by promoting COX activity and ATP production, via collaboration with isoform 1 in increasing protein levels of PINK1.
Alternative names
MMAC1, TEP1, PTEN, Inositol polyphosphate 3-phosphatase, Mutated in multiple advanced cancers 1, Phosphatase and tensin homolog
Recommended products
Rabbit Recombinant Monoclonal PTEN phospho T366 antibody. Suitable for IHC-P, Dot, WB and reacts with Human, Mouse, Rat, Synthetic peptide samples. Cited in 13 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP229
- Purification technique
- Affinity purification Protein A
- Specificity
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Stable for 12 months at -20°C
Notes
This product has switched from a hybridoma to recombinant production method on 9th June 2023.
PTEN is a protein implicated in several disease, including certain cancers and neurological diseases. PTEN is expressed ubiquitously throughout the body and acts as a phosphatase to dephosphorylate phosphatidylinositol (3,4,5)-trisphosphate. This is important in the inhibition of the Akt signalling pathway, which plays an important role in regulating cellular behaviours such as cell growth, survival, and migration.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The PTEN protein also known as phosphatase and tensin homolog is a phosphatase enzyme with a molecular mass of approximately 47 kDa. It acts mechanically by removing phosphate groups from phosphatidylinositol (345)-trisphosphate (PIP3) converting it to phosphatidylinositol (45)-bisphosphate. PTEN is ubiquitously expressed in various tissues with pronounced presence in the brain lung kidney and testis. This enzyme is an important regulator of cellular functions through its impact on signaling pathways.
- Biological function summary
PTEN plays important roles in cellular processes like apoptosis cell proliferation and migration. It negatively regulates the PI3K/AKT signaling pathway critical for cell survival and growth. PTEN is not part of a larger protein complex but interacts with various other proteins modulating its activity. It maintains cellular homeostasis by balancing growth-promoting signals with cell cycle arrest and apoptotic pathways.
- Pathways
PTEN is an important component in the PI3K/AKT and mTOR signaling pathways. These pathways regulate cell growth metabolism and survival and are interlinked with insulin signaling and cancer progression. PTEN's function directly interacts with proteins such as AKT and mTOR serving as a checkpoint that ensures controlled cellular proliferation. This positions PTEN as a tumor suppressor inhibiting uncontrolled cell growth via modulation of these pathways.
- Associated diseases and disorders
PTEN mutations or deletions are strongly associated with various types of cancers including breast and prostate cancer. PTEN interaction with the PI3K/AKT pathway influences cancer development often through loss-of-function mutations leading to unrestrained cellular growth. Beyond cancer PTEN mutations also relate to neurological disorders like Autism Spectrum Disorder where it affects signaling pathways involving proteins like mTOR. Understanding PTEN's role aids in unravelling the mechanistic underpinnings of these diseases paving the way for targeted therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN (phospho T366) antibody [EP229] (ab109454)
ab109454 at 1/100 dilution staining PTEN in Human breast ductal carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Western blot - Anti-PTEN (phospho T366) antibody [EP229] (ab109454), expandable thumbnail](https://content.abcam.com/products/images/pten-phospho-t366-antibody-ep229-ab109454--western-blot-img408368.jpg "Western blot - Anti-PTEN (phospho T366) antibody [EP229] (ab109454)")
Western blot - Anti-PTEN (phospho T366) antibody [EP229] (ab109454)
This data was developed using the same antibody clone in a different buffer formulation - BSA and Azide free (Anti-PTEN (phospho T366) antibody [EP229] - BSA and Azide free ab208104).
Recommended concentration for ab109454: 1/10000 dilution (0.1ug/ml).
Blocking and diluting buffer and concentration: 5% NFDM/TBST,
Primary and secondary antibodies were incubated for 1h at room temperature.All lanes: Western blot - Anti-PTEN (phospho T366) antibody [EP229] - BSA and Azide free (Anti-PTEN (phospho T366) antibody [EP229] - BSA and Azide free ab208104) at 1/100000 dilution
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg
Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) treated with 100ng/ml Calyculin A for 30 minutes whole cell lysate at 15 µg
Lane 3: HeLa (Human cervix adenocarcinoma epithelial cell) treated with 100ng/ml Calyculin A for 30 minutes whole cell lysate, then the membrane was incubated with Alkaline phosphatase at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Exposure time: 80s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN (phospho T366) antibody [EP229] (ab109454), expandable thumbnail](https://content.abcam.com/products/images/pten-phospho-t366-antibody-ep229-ab109454--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408371.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN (phospho T366) antibody [EP229] (ab109454)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN (phospho T366) antibody [EP229] (ab109454)
This data was developed using the same antibody clone in a different buffer formulation - BSA and Azide free (Anti-PTEN (phospho T366) antibody [EP229] - BSA and Azide free ab208104).
Recommended concentration for ab109454: 1/200 dilution.
The human prostate cancer sections were performed by Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins, then treated with or without alkaline phosphatase. After alkaline phosphatase treatment, the sections were labelled with Anti-PTEN (phospho T366) antibody [EP229] - BSA and Azide free ab208104 at 1/2000 dilution for 30 mins at room temperature. The Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) were used as secondary antibody. The sections Counterstained with hematoxylin. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Human prostate cancer without alkaline phosphatase treatment (image A), no signal was detected when treated with alkaline phosphatase (image B).
![Dot Blot - Anti-PTEN (phospho T366) antibody [EP229] (ab109454), expandable thumbnail](https://content.abcam.com/products/images/pten-phospho-t366-antibody-ep229-ab109454--dot-blot-img408372.jpg "Dot Blot - Anti-PTEN (phospho T366) antibody [EP229] (ab109454)")
Dot Blot - Anti-PTEN (phospho T366) antibody [EP229] (ab109454)
This data was developed using the same antibody clone in a different buffer formulation - BSA and Azide free (Anti-PTEN (phospho T366) antibody [EP229] - BSA and Azide free ab208104).
Recommended concentration for ab109454: 1/1000 dilution.
Dot blot analysis of PTEN (pT366) peptide (Lane 1), and PTEN non-phospho peptide (Lane 2) incubated with Anti-PTEN (phospho T366) antibody [EP229] - BSA and Azide free ab208104 at a dilution of 1/10000.
Goat Anti-Rabbit IgG H&L (HRP) ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100,000.Exposure time: 180s
![Western blot - Anti-PTEN (phospho T366) antibody [EP229] (ab109454), expandable thumbnail](https://content.abcam.com/products/images/pten-phospho-t366-antibody-ep229-ab109454--western-blot-img408370.jpg "Western blot - Anti-PTEN (phospho T366) antibody [EP229] (ab109454)")
Western blot - Anti-PTEN (phospho T366) antibody [EP229] (ab109454)
This data was developed using the same antibody clone in a different buffer formulation - BSA and Azide free (Anti-PTEN (phospho T366) antibody [EP229] - BSA and Azide free ab208104).
Recommended concentration for ab109454: 1/10000 dilution (0.1ug/ml).
Blocking and diluting buffer and concentration: 5% NFDM/TBST,
Primary and secondary antibodies were incubated for 1h at room temperature.All lanes: Western blot - Anti-PTEN (phospho T366) antibody [EP229] - BSA and Azide free (Anti-PTEN (phospho T366) antibody [EP229] - BSA and Azide free ab208104) at 1/100000 dilution
Lane 1: C6 (Rat glial tumor glial cell) whole cell lysate at 15 µg
Lane 2: C6 (Rat glial tumor glial cell) treated with 100 ng/ml Calyculin A for 30 minutes whole cell lysate at 15 µg
Lane 3: C6 (Rat glial tumor glial cell) treated with 100 ng/ml Calyculin A for 30 minutes whole cell lysate, then the membrane was incubated with Alkaline phosphatase at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Exposure time: 20s
![Western blot - Anti-PTEN (phospho T366) antibody [EP229] (ab109454), expandable thumbnail](https://content.abcam.com/products/images/pten-phospho-t366-antibody-ep229-ab109454--western-blot-img408369.jpg "Western blot - Anti-PTEN (phospho T366) antibody [EP229] (ab109454)")
Western blot - Anti-PTEN (phospho T366) antibody [EP229] (ab109454)
This data was developed using the same antibody clone in a different buffer formulation - BSA and Azide free (Anti-PTEN (phospho T366) antibody [EP229] - BSA and Azide free ab208104).
Recommended concentration for ab109454: 1/10000 dilution (0.1ug/ml).
Blocking and diluting buffer and concentration: 5% NFDM/TBST,
Primary and secondary antibodies were incubated for 1h at room temperature.Lanes 1 - 3: Western blot - Anti-PTEN (phospho T366) antibody [EP229] - BSA and Azide free (Anti-PTEN (phospho T366) antibody [EP229] - BSA and Azide free ab208104) at 1/100000 dilution
Lane 1: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 15 µg
Lane 2: NIH/3T3 (Mouse embryonic fibroblast) treated with 100 ng/ml Calyculin A for 30 minutes whole cell lysate at 15 µg
Lane 3: NIH/3T3 (Mouse embryonic fibroblast) treated with 100 ng/ml Calyculin A for 30 minutes whole cell lysate, then the membrane was incubated with Alkaline phosphatase at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Exposure time: 40s
![Western blot - Anti-PTEN (phospho T366) antibody [EP229] (ab109454), expandable thumbnail](https://content.abcam.com/products/images/pten-phospho-t366-antibody-ep229-ab109454--western-blot-img408369.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PTEN (phospho T366) antibody [EP229] (ab109454)")
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