AB182398

Anti-PTF1A antibody [EPR19011]

Name: Anti-PTF1A antibody [EPR19011] (ab182398) | Abcam
Brand: Abcam Limited
SKU: AB182398
Price: 4865 USD
Availability: InStock
Rating: 3 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(1 Publication)

Rabbit Recombinant Monoclonal PTF1A antibody. Suitable for IP, WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

View Alternative Names

BHLHA29, PTF1P48, PTF1A, Pancreas transcription factor 1 subunit alpha, Class A basic helix-loop-helix protein 29, Pancreas-specific transcription factor 1a, bHLH transcription factor p48, p48 DNA-binding subunit of transcription factor PTF1, bHLHa29, PTF1-p48

4 Images

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Immunoprecipitation - Anti-PTF1A antibody [EPR19011] (AB182398)

PTF1A was immunoprecipitated from 1 mg of NIH/3T3 (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab182398 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab182398 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : NIH/3T3 whole cell lysate 10 μg (Input).

Lane 2 : ab182398 IP in NIH/3T3 whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab182398 in NIH/3T3 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-PTF1A antibody [EPR19011] (ab182398)

Predicted band size: 35 kDa,83 kDa

Observed band size: 48 kDa

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Supplier Data

Western blot - Anti-PTF1A antibody [EPR19011] (AB182398)

Blocking/Dilution buffer : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 18347078).

All lanes:

Western blot - Anti-PTF1A antibody [EPR19011] (ab182398) at 1/2000 dilution

Lane 1:

Human fetal heart tissue lysate at 20 µg

Lane 2:

Human fetal kidney tissue lysate at 20 µg

Lane 3:

BxPC-3 (Human pancreas adenocarcinoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/10000 dilution

Predicted band size: 35 kDa,58 kDa

Observed band size: 42 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-PTF1A antibody [EPR19011] (AB182398)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1 : 3 minutes; Lane 2 : 30 seconds; Lane 3, 4, 5 and 6 : 10 seconds.

All lanes:

Western blot - Anti-PTF1A antibody [EPR19011] (ab182398) at 1/2000 dilution

Lane 1:

Rat heart tissue lysate at 20 µg

Lane 2:

Rat pancreas tissue lysate at 20 µg

Lane 3:

C6 (Rat glial tumor cell line) whole cell lysate at 10 µg

Lane 4:

RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 5:

PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg

Lane 6:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 35 kDa,67 kDa

Observed band size: 42 kDa

false

Supplier Data

Western blot - Anti-PTF1A antibody [EPR19011] (AB182398)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PTF1A antibody [EPR19011] (ab182398) at 1/5000 dilution

All lanes:

Mouse pancreas lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 35 kDa

Observed band size: 42 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19011

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PTF1A also known as pancreas specific transcription factor 1a or PTFL is a bHLH (basic Helix-Loop-Helix) transcription factor with a mass of approximately 35 kDa. This protein functions as a DNA-binding protein that regulates gene expression. It is mainly expressed in the pancreas especially within pancreatic progenitor cells and also in certain neural tissues. PTF1A is necessary for the transcriptional activity of genes involved in pancreatic development.

Biological function summary

The PTF1A protein plays a significant role in pancreas development and maintenance. It forms a complex as part of the pancreas transcription factor 1 (PTF1) complex which includes other proteins like RBPJL and the E-box binding protein. This complex binds to specific regulatory regions of DNA to modulate the expression of genes important for acinar cell differentiation and function in the pancreas.

Pathways

PTF1A operates within the Notch signaling pathway and the Wnt signaling pathway. In the Notch pathway PTF1A interacts with proteins such as Notch receptors and target HES genes to influence cell differentiation. Within the Wnt pathway PTF1A impacts genes involved with cell fate decisions during pancreatic development working with signaling proteins like beta-catenin to mediate its effects.

PTF1A mutations are associated with pancreatic agenesis and cerebellar atrophy. Pancreatic agenesis involves the underdevelopment or absence of the pancreas resulting in glucose homeostasis complications. PTF1A can interact with genes such as PDX1 in this context which is another transcription factor involved in pancreatic development. In cerebellar atrophy the disruptions in PTF1A expression can lead to problems with cerebellar development causing neurological symptoms due to its interaction with neural development pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor implicated in the cell fate determination in various organs. Binds to the E-box consensus sequence 5'-CANNTG-3'. Plays a role in early and late pancreas development and differentiation. Important for determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. May be involved in the maintenance of exocrine pancreas-specific gene expression including ELA1 and amylase. Required for the formation of pancreatic acinar and ductal cells. Plays an important role in cerebellar development. Directly regulated by FOXN4 and RORC during retinal development, FOXN4-PTF1A pathway plays a central role in directing the differentiation of retinal progenitors towards horizontal and amacrine fates.

See full target information PTF1A

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of neuroscience : the official journal of the Society for Neuroscience 32:10352-64 PubMed22836268

2012

ARF6 directs axon transport and traffic of integrins and regulates axon growth in adult DRG neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Richard Eva,Sarah Crisp,Jamie R K Marland,Jim C Norman,Venkateswarlu Kanamarlapudi,Charles ffrench-Constant,James W Fawcett

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PTF1A antibody [EPR19011]

Immunoprecipitation - Anti-PTF1A antibody [EPR19011] (AB182398)

Western blot - Anti-PTF1A antibody [EPR19011] (AB182398)

Western blot - Anti-PTF1A antibody [EPR19011] (AB182398)

Western blot - Anti-PTF1A antibody [EPR19011] (AB182398)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise