AB229961

Anti-PTGES2/Gbf1 antibody

Name: Anti-PTGES2/Gbf1 antibody (ab229961) | Abcam
Brand: Abcam Limited
SKU: AB229961
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal PTGES2/Gbf1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PTGES2 aa 50 to C-terminus.

View Alternative Names

C9orf15, PGES2, PTGES2, Prostaglandin E synthase 2, Membrane-associated prostaglandin E synthase-2, Microsomal prostaglandin E synthase 2, Prostaglandin-H(2) E-isomerase, mPGE synthase-2, mPGES-2

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTGES2/Gbf1 antibody (AB229961)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTGES2/Gbf1 antibody (AB229961)

Paraffin-embedded human colon cancer tissue stained for PTGES2/Gbf1 using ab229961 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTGES2/Gbf1 antibody (AB229961)

Paraffin-embedded human placenta tissue stained for PTGES2/Gbf1 using ab229961 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-PTGES2/Gbf1 antibody (AB229961)

All lanes:

Western blot - Anti-PTGES2/Gbf1 antibody (ab229961) at 1/1000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 3:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to Rabbit IgG at 1/10000 dilution

Predicted band size: 42 kDa

Observed band size: 42 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human PTGES2 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9H7Z7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PTGES2 also known as Gbf1 is a member of the prostaglandin E synthase family and functions as an enzyme involved in the conversion of PGH2 to PGE2 an essential step in prostaglandin biosynthesis. The protein has an approximate mass of 40 kDa. PTGES2 is expressed across various tissues with higher levels observed in the brain kidney and liver. The enzyme operates optimally in the presence of reduced glutathione which is necessary for its activity.

Biological function summary

PTGES2 plays an important role in the synthesis of prostaglandins which are lipid compounds that perform critical functions in inflammation and other cellular processes. As part of the prostaglandin synthesis pathway PTGES2 interacts cooperatively with other enzymes such as cyclooxygenase-2 (COX-2) to produce PGE2. PGE2 is significant for regulating immune responses and maintaining homeostasis. Disruptions in prostaglandin production can lead to various inflammatory disorders.

Pathways

PTGES2 is integral to the arachidonic acid metabolic pathway where it acts in unison with COX-2 and other enzymes to convert arachidonic acid into bioactive prostaglandins. Specifically its role in the PGE2 synthesis pathway is well-documented which ties it closely to proteins involved in eicosanoid synthesis. PGE2 the product of this pathway modulates both pain and inflammation showing how PTGES2 impacts physiological processes beyond its enzymatic function.

PTGES2 has been linked to inflammatory conditions such as rheumatoid arthritis and cancer. Its activity correlates with increased levels of PGE2 a molecule known for promoting inflammation and tumorigenesis. Associations with COX-2 have been studied extensively in the context of cancer especially in relation to its impact on cellular proliferation and immune evasion. As our understanding of PTGES2 continues to evolve its potential as a therapeutic target for inflammatory and neoplastic diseases is being actively investigated.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Isomerase that catalyzes the conversion of PGH2 into the more stable prostaglandin E2 (PGE2) (in vitro) (PubMed : 12804604, PubMed : 17585783, PubMed : 18198127). The biological function and the GSH-dependent property of PTGES2 is still under debate (PubMed : 17585783, PubMed : 18198127). In vivo, PTGES2 could form a complex with GSH and heme and would not participate in PGE2 synthesis but would catalyze the degradation of prostaglandin E2 H2 (PGH2) to 12(S)-hydroxy-5(Z),8(E),10(E)-heptadecatrienoic acid (HHT) and malondialdehyde (MDA) (By similarity) (PubMed : 17585783).

See full target information PTGES2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Discover. Oncology 14:108 PubMed37351805

2023

Development and validation of a predictive model in diagnosis and prognosis of primary glioblastoma patients based on Homeobox A family.

Applications

Unspecified application

Species

Unspecified reactive species

Zong-Qing Zheng,Gui-Qiang Yuan,Guo-Guo Zhang,Qian-Qian Nie,Zhong Wang

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PTGES2/Gbf1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTGES2/Gbf1 antibody (AB229961)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTGES2/Gbf1 antibody (AB229961)

Western blot - Anti-PTGES2/Gbf1 antibody (AB229961)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

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