AB97567

Anti-PTGFRN antibody

Name: Anti-PTGFRN antibody (ab97567) | Abcam
Brand: Abcam Limited
SKU: AB97567
Price: 535 USD
Availability: InStock
Rating: 1 (1 reviews)

(1 Review)

(6 Publications)

Rabbit Polyclonal PTGFRN antibody. Suitable for ICC/IF, IHC-P, WB and reacts with Human, Rat samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human PTGFRN aa 800 to C-terminus.

View Alternative Names

CD315, CD9P1, EWIF, FPRP, KIAA1436, PTGFRN, Prostaglandin F2 receptor negative regulator, CD9 partner 1, Glu-Trp-Ile EWI motif-containing protein F, Prostaglandin F2-alpha receptor regulatory protein, Prostaglandin F2-alpha receptor-associated protein, CD9P-1, EWI-F

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-PTGFRN antibody (AB97567)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PTGFRN antibody (AB97567)

Immunofluorescence analysis of methanol-fixed A431, using ab97567 at 1/500 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTGFRN antibody (AB97567)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTGFRN antibody (AB97567)

Immunohistochemical analysis of paraffin-embedded human normal oral tissue, using ab97567 at 1/100 dilution.

Antigen Retrieval : Trilogy™ (EDTA based, pH 8.0) buffer, 15min

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTGFRN antibody (AB97567)

Immunohistochemical analysis of paraffin-embedded rat duodenum tissue, using ab97567 at 1/400 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTGFRN antibody (AB97567)

Immunohistochemical analysis of paraffin-embedded rat brain tissue, using ab97567 at 1/400 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

Supplier Data

Western blot - Anti-PTGFRN antibody (AB97567)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-PTGFRN antibody (ab97567) at 1/2000 dilution

Lane 1:

A549 whole cell lysates at 30 µg

Lane 2:

H1299 whole cell lysates at 30 µg

Lane 3:

HCT116 whole cell lysates at 30 µg

Predicted band size: 99 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human PTGFRN aa 800 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9P2B2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PTGFRN also known as Prostaglandin F2 receptor negative regulator is a membrane protein with a mass of approximately 94 kDa. This protein is expressed on various cell types including immune cells like monocytes and macrophages as well as in the heart and lung tissues. PTGFRN interacts with other membrane proteins to modify signalling pathways particularly those involving G-protein-coupled receptors.

Biological function summary

PTGFRN modulates prostaglandin-mediated signaling serving as a regulatory component that dampens the response to prostaglandin F2 alpha. It is part of a multi-protein complex which includes interactions with proteins like CD9 and integrins. These interactions influence cell adhesion and mobility making PTGFRN relevant in processes like inflammation and tissue repair.

Pathways

PTGFRN plays a significant role in the prostaglandin signaling cascade affecting signal transduction linked with inflammatory responses. It is closely associated with the PI3K/Akt pathway a critical pathway for cellular survival and proliferation. The interactions between PTGFRN and CD9 or integrins facilitate the integration of signals that modify cell growth and immune function.

PTGFRN has been implicated in heart failure and inflammatory lung diseases like chronic obstructive pulmonary disease (COPD). In heart failure PTGFRN can interact with integrins to influence cardiac remodeling and fibrosis. In COPD its regulatory function within the prostaglandin pathway affects inflammatory responses. Understanding these interactions provides insights into potential therapeutic targets for managing these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Inhibits the binding of prostaglandin F2-alpha (PGF2-alpha) to its specific FP receptor, by decreasing the receptor number rather than the affinity constant. Functional coupling with the prostaglandin F2-alpha receptor seems to occur (By similarity). In myoblasts, associates with tetraspanins CD9 and CD81 to prevent myotube fusion during muscle regeneration (By similarity).

See full target information PTGFRN

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of extracellular vesicles 13:e70012 PubMed39508411

2024

PlexinA1 (PLXNA1) as a novel scaffold protein for the engineering of extracellular vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Hang Zhao,Zhi Li,Da Liu,Jiaxun Zhang,Zhicheng You,Yuzhang Shao,Hongyan Li,Jun Yang,Xiang Liu,Miaozhu Wang,Chengen Wu,Jing Chen,Jianwu Wang,Guanyi Kong,Libo Zhao

Bioactive materials 32:488-501 PubMed37965241

2023

Profibrogenic macrophage-targeted delivery of mitochondrial protector via exosome formula for alleviating pulmonary fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zhang,Zhuo Wan,Di Qu,Wenqi Sun,Liang Zhang,Yuan Liang,Lei Pan,Hua Jiang,Zichen Ye,Mengying Wei,Lijun Yuan,Guodong Yang,Faguang Jin

BMC cancer 22:642 PubMed35690717

2022

Integrative analysis of cell adhesion molecules in glioblastoma identified prostaglandin F2 receptor inhibitor (PTGFRN) as an essential gene.

Applications

Unspecified application

Species

Unspecified reactive species

Uchurappa Mala,Tapan Kumar Baral,Kumaravel Somasundaram

International journal of molecular medicine 47: PubMed33760118

2021

Knockdown of long non‑coding RNA DLEU2 suppresses idiopathic pulmonary fibrosis by regulating the microRNA‑369‑3p/TRIM2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Hengzhong Yi,Danlin Luo,Yangbao Xiao,Di Jiang

Cancer letters 462:33-42 PubMed31377205

2019

The Ig superfamily protein PTGFRN coordinates survival signaling in glioblastoma multiforme.

Applications

Unspecified application

Species

Unspecified reactive species

Brittany Aguila,Adina Brett Morris,Raffaella Spina,Eli Bar,Julie Schraner,Robert Vinkler,Jason W Sohn,Scott M Welford

Journal of virology 85:2781-92 PubMed21228236

2011

Rotavirus replication requires a functional proteasome for effective assembly of viroplasms.

Applications

Unspecified application

Species

Unspecified reactive species

R Contin,F Arnoldi,M Mano,O R Burrone

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PTGFRN antibody

Immunocytochemistry/ Immunofluorescence - Anti-PTGFRN antibody (AB97567)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTGFRN antibody (AB97567)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTGFRN antibody (AB97567)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTGFRN antibody (AB97567)

Western blot - Anti-PTGFRN antibody (AB97567)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise