Rabbit Polyclonal PTGIS/PGIS antibody. Suitable for WB, IHC-P, ICC/IF, IHC-Fr and reacts with Mouse, Rat, Human, Cow, Sheep samples. Cited in 16 publications.
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- JCO precision oncology 7:e22006302023Key Molecules in Bladder Cancer Affect Patient Prognosis and Immunotherapy Efficacy: Further Exploration for and .Applications:Unspecified applicationReactive species:Unspecified reactive speciesTailai Zhou,Hengxin Chen,Yinzhao Wang,Sijie Wen,Pinghong Dao,Minfeng ChenPubMed 37437228
- Physiological reports 10:e155052022Identification of exercise-regulated genes in mice exposed to cigarette smoke.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLars Aakerøy,Chew W Cheng,Pavla Sustova,Nathan R Scrimgeour,Sissel Gyrid Freim Wahl,Sigurd Steinshamn,T Scott Bowen,Eivind BrønstadPubMed 36324300
- Frontiers in endocrinology 13:9423682022Identification and analysis of novel endometriosis biomarkers integrative bioinformatics.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSung-Jin Bae,Yunju Jo,Min Kyoung Cho,Jung-Sook Jin,Jin-Young Kim,Jaewon Shim,Yun Hak Kim,Jang-Kyung Park,Dongryeol Ryu,Hyun Joo Lee,Jongkil Joo,Ki-Tae HaPubMed 36339397
- Frontiers in oncology 11:7195642021Single-Cell RNA Sequencing in Multiple Pathologic Types of Renal Cell Carcinoma Revealed Novel Potential Tumor-Specific Markers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCheng Su,Yufang Lv,Wenhao Lu,Zhenyuan Yu,Yu Ye,Bingqian Guo,Deyun Liu,Haibiao Yan,Tianyu Li,Qingyun Zhang,Jiwen Cheng,Zengnan MoPubMed 34722263
- Animals : an open access journal from MDPI 11:2021Hypothyroidism Affects Uterine Function via the Modulation of Prostaglandin Signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIlona Kowalczyk-Zieba,Joanna Staszkiewicz-Chodor,Dorota Boruszewska,Krzysztof Lukaszuk,Joanna Jaworska,Izabela Woclawek-PotockaPubMed 34573602
- Laboratory investigation; a journal of technical methods and pathology 101:1210-12242021Alternative activation of macrophages by prostacyclin synthase ameliorates alcohol induced liver injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXue-Yin Pan,Ling Wang,Hong-Mei You,Miao Cheng,Yang Yang,Cheng Huang,Jun LiPubMed 34112940
- The EMBO journal 39:e1034542020Tissue-resident macrophages actively suppress IL-1beta release via a reactive prostanoid/IL-10 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNatacha Ipseiz,Robert J Pickering,Marcela Rosas,Victoria J Tyrrell,Luke C Davies,Selinda J Orr,Magdalena A Czubala,Dina Fathalla,Avril Ab Robertson,Clare E Bryant,Valerie O'Donnell,Philip R TaylorPubMed 32484988
- International journal of biological sciences 15:2037-20502019Vascular Protection of TPE-CA on Hyperhomocysteinemia-induced Vascular Endothelial Dysfunction through AA Metabolism Modulated CYPs Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui Li et. al.PubMed 31592228
- Chemico-biological interactions 298:104-1112018Pyrrolizidine alkaloid-induced alterations of prostanoid synthesis in human endothelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJohanna Ebmeyer,Jessica Behrend,Mario Lorenz,Georgia Günther,Raymond Reif,Jan G Hengstler,Albert Braeuning,Alfonso Lampen,Stefanie Hessel-PrasPubMed 30465738
- Journal of oncology 2018:63019802018Differential Expression of Prostaglandin I2 Synthase Associated with Arachidonic Acid Pathway in the Oral Squamous Cell Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnelise Russo,Patrícia M Biselli-Chicote,Rosa S Kawasaki-Oyama,Márcia M U Castanhole-Nunes,José V Maniglia,Dalísio de Santi Neto,Érika C Pavarino,Eny M Goloni-BertolloPubMed 30532780
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
- Standard buffer
- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information. Database link O35074
Reactivity data
Select an application| WB | IHC-P | ICC/IF | IHC-Fr | |
|---|---|---|---|---|
| Human | Expected | Tested | Expected | Expected |
| Mouse | Expected | Predicted | Expected | Expected |
| Rat | Expected | Predicted | Expected | Expected |
| Cow | Expected | Predicted | Expected | Expected |
| Sheep | Expected | Predicted | Expected | Expected |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Cow, Sheep | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Cow, Rat, Mouse | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Cow, Sheep | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-Fr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Cow, Sheep | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Catalyzes the biosynthesis and metabolism of eicosanoids. Catalyzes the isomerization of prostaglandin H2 to prostacyclin (= prostaglandin I2), a potent mediator of vasodilation and inhibitor of platelet aggregation. Additionally, displays dehydratase activity, toward hydroperoxyeicosatetraenoates (HPETEs), especially toward (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate (15(S)-HPETE).
Alternative names
Cyp8, Prostacyclin synthase, Hydroperoxy icosatetraenoate dehydratase, Prostaglandin I2 synthase
Recommended products
Rabbit Polyclonal PTGIS/PGIS antibody. Suitable for WB, IHC-P, ICC/IF, IHC-Fr and reacts with Mouse, Rat, Human, Cow, Sheep samples. Cited in 16 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
- Standard buffer
- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information. Database link O35074
- Concentration
- Purification notes
Affinity purified
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein PTGIS also known as prostacyclin synthase (PGIS) is an enzyme involved in the conversion of prostaglandin H2 (PGH2) to prostacyclin (PGI2) a member of the prostaglandin family. It has a molecular mass of approximately 56 kDa. PTGIS is mainly expressed in vascular endothelial cells as well as in other tissues including heart kidney and lung. The protein is important for various physiological functions due to its enzymatic role in prostacyclin synthesis.
- Biological function summary
PTGIS plays a role in vasodilation and inhibiting platelet aggregation. Prostacyclin the product of PTGIS activity functions as a potent vasodilator and inhibitor of platelet activation which are key processes for maintaining vascular health. PTGIS does not form part of a larger protein complex but works independently to generate its product PGI2. By performing these roles PTGIS contributes significantly to cardiovascular homeostasis.
- Pathways
PTGIS is an important component of the prostaglandin biosynthesis pathway. This pathway is important for the formation of several types of prostaglandins which are involved in various aspects of inflammation and vascular function. PTGIS connects closely with cyclooxygenase (COX) enzymes specifically COX-1 and COX-2 which generate PGH2 from arachidonic acid. The interaction with COX enzymes is necessary for the effective production of prostacyclin.
- Associated diseases and disorders
PTGIS has implications in thrombotic diseases and pulmonary hypertension. The enzyme's role in inhibiting platelet aggregation makes it relevant in conditions where clot formation is a risk such as cardiovascular disorders. Its relation to pulmonary hypertension involves dysregulation of prostacyclin production which affects blood pressure in lung arteries. Additionally interactions with proteins like thromboxane synthase which counteracts the effects of prostacyclin are important for understanding the balance between factors promoting and inhibiting thrombosis in these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTGIS/PGIS antibody (ab23668)
ab23668 (4μg/ml) staining PTGIS/PGIS in human adrenal gland using an automated system (DAKO Autostainer Plus). Using this protocol there is apical cytoplasmic/ endoplasmic reticulum staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
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