AB23668

Anti-PTGIS/PGIS antibody

Name: Anti-PTGIS/PGIS antibody (ab23668) | Abcam
Brand: Abcam Limited
SKU: AB23668
Rating: 5 (2 reviews)

(2 Reviews)

(19 Publications)

Rabbit Polyclonal PTGIS/PGIS antibody. Suitable for WB, IHC-P, ICC/IF, IHC-Fr and reacts with Mouse, Rat, Human, Cow, Sheep samples. Cited in 19 publications. Immunogen corresponding to Synthetic Peptide within Mouse Ptgis aa 450-500.

View Alternative Names

Cyp8, Prostacyclin synthase, Hydroperoxy icosatetraenoate dehydratase, Prostaglandin I2 synthase

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTGIS/PGIS antibody (AB23668)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTGIS/PGIS antibody (AB23668)

ab23668 (4μg/ml) staining PTGIS/PGIS in human adrenal gland using an automated system (DAKO Autostainer Plus). Using this protocol there is apical cytoplasmic/ endoplasmic reticulum staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Sheep, Cow, Rat, Mouse

Applications

WB, IHC-P, IHC-Fr, ICC/IF

applications

Immunogen

Synthetic Peptide within Mouse Ptgis aa 450-500. The exact immunogen used to generate this antibody is proprietary information.

O35074

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Reactivity data

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Properties and storage information

Form

Liquid

Purification notes

Affinity purified

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein PTGIS also known as prostacyclin synthase (PGIS) is an enzyme involved in the conversion of prostaglandin H2 (PGH2) to prostacyclin (PGI2) a member of the prostaglandin family. It has a molecular mass of approximately 56 kDa. PTGIS is mainly expressed in vascular endothelial cells as well as in other tissues including heart kidney and lung. The protein is important for various physiological functions due to its enzymatic role in prostacyclin synthesis.

Biological function summary

PTGIS plays a role in vasodilation and inhibiting platelet aggregation. Prostacyclin the product of PTGIS activity functions as a potent vasodilator and inhibitor of platelet activation which are key processes for maintaining vascular health. PTGIS does not form part of a larger protein complex but works independently to generate its product PGI2. By performing these roles PTGIS contributes significantly to cardiovascular homeostasis.

Pathways

PTGIS is an important component of the prostaglandin biosynthesis pathway. This pathway is important for the formation of several types of prostaglandins which are involved in various aspects of inflammation and vascular function. PTGIS connects closely with cyclooxygenase (COX) enzymes specifically COX-1 and COX-2 which generate PGH2 from arachidonic acid. The interaction with COX enzymes is necessary for the effective production of prostacyclin.

PTGIS has implications in thrombotic diseases and pulmonary hypertension. The enzyme's role in inhibiting platelet aggregation makes it relevant in conditions where clot formation is a risk such as cardiovascular disorders. Its relation to pulmonary hypertension involves dysregulation of prostacyclin production which affects blood pressure in lung arteries. Additionally interactions with proteins like thromboxane synthase which counteracts the effects of prostacyclin are important for understanding the balance between factors promoting and inhibiting thrombosis in these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the biosynthesis and metabolism of eicosanoids. Catalyzes the isomerization of prostaglandin H2 to prostacyclin (= prostaglandin I2), a potent mediator of vasodilation and inhibitor of platelet aggregation. Additionally, displays dehydratase activity, toward hydroperoxyeicosatetraenoates (HPETEs), especially toward (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate (15(S)-HPETE).

See full target information Ptgis

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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Journal of the American Heart Association 13:e032872 PubMed38639351

2024

Identification of Rare Variants in Patients With Williams Syndrome and Severe Peripheral Pulmonary Stenosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ayako Chida-Nagai,Hiroyuki Akagawa,Saori Sawai,Yue-Jiao Ma,Satoshi Yakuwa,Jun Muneuchi,Kazushi Yasuda,Hirokuni Yamazawa,Toshiyuki Yamamoto,Emi Takakuwa,Utano Tomaru,Yoshiyuki Furutani,Tatsuya Kato,Gen Harada,Kei Inai,Toshio Nakanishi,Atsushi Manabe,Atsuhito Takeda,Zhi-Cheng Jing

Cells 13: PubMed38334598

2024

Blastocyst-Derived Lactic Acid May Regulate S100A6 Expression and Function in Mouse Decidualization via Stimulation of Uterine Epithelial Arachidonic Acid Secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Meng-Yuan Li,Ying Wang,Ying Wu,Xu-Yu Zhao,Zhen-Shan Yang,Bo Li,Si-Ting Chen,Yu-Ying He,Zeng-Ming Yang

Journal of the American Society of Nephrology : JASN 35:149-165 PubMed38062563

2023

Prostacyclin Mitigates Renal Fibrosis by Activating Fibroblast Prostaglandin I 2 Receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Li,Yi Guan,Yunyu Xu,Yingxue Cao,Qionghong Xie,Raymond C Harris,Matthew D Breyer,Limin Lu,Chuan-Ming Hao

JCO precision oncology 7:e2200630 PubMed37437228

2023

Key Molecules in Bladder Cancer Affect Patient Prognosis and Immunotherapy Efficacy: Further Exploration for and .

Applications

Unspecified application

Species

Unspecified reactive species

Tailai Zhou,Hengxin Chen,Yinzhao Wang,Sijie Wen,Pinghong Dao,Minfeng Chen

Physiological reports 10:e15505 PubMed36324300

2022

Identification of exercise-regulated genes in mice exposed to cigarette smoke.

Applications

Unspecified application

Species

Unspecified reactive species

Lars Aakerøy,Chew W Cheng,Pavla Sustova,Nathan R Scrimgeour,Sissel Gyrid Freim Wahl,Sigurd Steinshamn,T Scott Bowen,Eivind Brønstad

Frontiers in endocrinology 13:942368 PubMed36339397

2022

Identification and analysis of novel endometriosis biomarkers integrative bioinformatics.

Applications

Unspecified application

Species

Unspecified reactive species

Sung-Jin Bae,Yunju Jo,Min Kyoung Cho,Jung-Sook Jin,Jin-Young Kim,Jaewon Shim,Yun Hak Kim,Jang-Kyung Park,Dongryeol Ryu,Hyun Joo Lee,Jongkil Joo,Ki-Tae Ha

Frontiers in oncology 11:719564 PubMed34722263

2021

Single-Cell RNA Sequencing in Multiple Pathologic Types of Renal Cell Carcinoma Revealed Novel Potential Tumor-Specific Markers.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Su,Yufang Lv,Wenhao Lu,Zhenyuan Yu,Yu Ye,Bingqian Guo,Deyun Liu,Haibiao Yan,Tianyu Li,Qingyun Zhang,Jiwen Cheng,Zengnan Mo

Animals : an open access journal from MDPI 11: PubMed34573602

2021

Hypothyroidism Affects Uterine Function via the Modulation of Prostaglandin Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Ilona Kowalczyk-Zieba,Joanna Staszkiewicz-Chodor,Dorota Boruszewska,Krzysztof Lukaszuk,Joanna Jaworska,Izabela Woclawek-Potocka

Laboratory investigation; a journal of technical methods and pathology 101:1210-1224 PubMed34112940

2021

Alternative activation of macrophages by prostacyclin synthase ameliorates alcohol induced liver injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xue-Yin Pan,Ling Wang,Hong-Mei You,Miao Cheng,Yang Yang,Cheng Huang,Jun Li

The EMBO journal 39:e103454 PubMed32484988

2020

Tissue-resident macrophages actively suppress IL-1beta release via a reactive prostanoid/IL-10 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Natacha Ipseiz,Robert J Pickering,Marcela Rosas,Victoria J Tyrrell,Luke C Davies,Selinda J Orr,Magdalena A Czubala,Dina Fathalla,Avril Ab Robertson,Clare E Bryant,Valerie O'Donnell,Philip R Taylor

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PTGIS/PGIS antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTGIS/PGIS antibody (AB23668)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification notes

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (19)

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