AB224503

Anti-PTHLH antibody

Name: Anti-PTHLH antibody (ab224503) | Abcam
Brand: Abcam Limited
SKU: AB224503
Rating: 4 (1 reviews)

(1 Review)

(2 Publications)

Rabbit Polyclonal PTHLH antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PTHLH aa 1-200.

View Alternative Names

PTHRP, PTHLH, Parathyroid hormone-related protein, PTH-rP, PTHrP, Parathyroid hormone-like protein, PLP

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTHLH antibody (AB224503)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTHLH antibody (AB224503)

Paraffin-embedded human breast tissue stained for PTHLH using ab224503 at 1/50 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-PTHLH antibody (AB224503)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PTHLH antibody (AB224503)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for PTHLH (green) using ab224503 at 4 μg/ml in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PTHLH aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

P12272

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Parathyroid hormone-like hormone (PTHLH) also known as PTHrP is a protein made by the PTHLH gene. It weighs around 17 kDa and its expression occurs in many tissues including the bone skin and developing structures during embryogenesis. PTHLH interacts with parathyroid hormone receptors offering actions similar to parathyroid hormone (PTH). This interaction helps to extend its role beyond classical calcium homeostasis to include other functions such as growth and developmental processes.

Biological function summary

PTHLH plays a role in cartilage development and endochondral bone formation. It functions as a significant regulator of cellular proliferation and differentiation especially in chondrocytes and osteoblasts. PTHLH can also act as part of multiprotein complexes that influence transcription and intracellular signaling. Furthermore it possesses diverse roles including smooth muscle contraction mammary gland development and cellular differentiation inhibition.

Pathways

Alongside related proteins like Indian Hedgehog (IHH) PTHLH resides in pathways critical for skeletal development and homeostasis. Notably the PTHLH/PTHR1 signaling pathway regulates chondrocyte maturation during bone growth. This pathway intersects with the Hedgehog pathway to form a regulatory loop essential for maintaining the growth plate's development and maturation. PTHLH also influences the Wnt signaling pathway associated with bone density and health.

PTHLH has connections with certain cancers specifically breast cancer and hypercalcemia of malignancy. In cancerous tissues PTHLH can be overexpressed leading to increased osteoclastic bone resorption contributing to cancer-associated conditions. Additionally PTHLH overactivity links it to familial benign hypercalcemia through its interactions with PTH and its receptors. Understanding these associations could offer therapeutic targets for treatment.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Neuroendocrine peptide which is a critical regulator of cellular and organ growth, development, migration, differentiation and survival and of epithelial calcium ion transport (PubMed : 12538599, PubMed : 35932760, PubMed : 3616618). Acts by binding to its receptor, PTH1R, activating G protein-coupled receptor signaling (PubMed : 19674967, PubMed : 35932760). Regulates endochondral bone development and epithelial-mesenchymal interactions during the formation of the mammary glands and teeth (By similarity). Required for skeletal homeostasis (PubMed : 12538599). Promotes mammary mesenchyme differentiation and bud outgrowth by modulating mesenchymal cell responsiveness to BMPs (PubMed : 12538599). Up-regulates BMPR1A expression in the mammary mesenchyme and this increases the sensitivity of these cells to BMPs and allows them to respond to BMP4 in a paracrine and/or autocrine fashion (By similarity). BMP4 signaling in the mesenchyme, in turn, triggers epithelial outgrowth and augments MSX2 expression, which causes the mammary mesenchyme to inhibit hair follicle formation within the nipple sheath (By similarity). Promotes colon cancer cell migration and invasion in an integrin alpha-6/beta-1-dependent manner through activation of Rac1 (PubMed : 20637541).. Osteostatin. Potent inhibitor of osteoclastic bone resorption.

See full target information PTHLH

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2206852 PubMed37083227

2023

Deciphering the Molecular Characteristics of Human Idiopathic Nonobstructive Azoospermia from the Perspective of Germ Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yidong Chen,Xixi Liu,Li Zhang,Feiyin Zhu,Liying Yan,Wenhao Tang,Zhe Zhang,Qiang Liu,Hui Jiang,Jie Qiao

Histochemistry and cell biology 154:397-403 PubMed32710187

2020

High endogenous expression of parathyroid hormone-related protein (PTHrP) supports osteogenic differentiation in human dental follicle cells.

Applications

Unspecified application

Species

Unspecified reactive species

Oliver Pieles,Anja Reck,Christian Morsczeck

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-PTHLH antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTHLH antibody (AB224503)

Immunocytochemistry/ Immunofluorescence - Anti-PTHLH antibody (AB224503)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise