Rabbit Polyclonal PTHLH antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PTHLH aa 1 to C-terminus.
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- Histochemistry and cell biology 154:397-4032020High endogenous expression of parathyroid hormone-related protein (PTHrP) supports osteogenic differentiation in human dental follicle cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOliver Pieles et. al.PubMed 32710187
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human PTHLH aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P12272
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Rabbit, Cow, Dog | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Rabbit, Cow, Dog | Dilution info - | Notes - |
Associated Products
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Target data
Function
Neuroendocrine peptide which is a critical regulator of cellular and organ growth, development, migration, differentiation and survival and of epithelial calcium ion transport. Regulates endochondral bone development and epithelial-mesenchymal interactions during the formation of the mammary glands and teeth. Required for skeletal homeostasis. Promotes mammary mesenchyme differentiation and bud outgrowth by modulating mesenchymal cell responsiveness to BMPs. Up-regulates BMPR1A expression in the mammary mesenchyme and this increases the sensitivity of these cells to BMPs and allows them to respond to BMP4 in a paracrine and/or autocrine fashion. BMP4 signaling in the mesenchyme, in turn, triggers epithelial outgrowth and augments MSX2 expression, which causes the mammary mesenchyme to inhibit hair follicle formation within the nipple sheath (By similarity). Promotes colon cancer cell migration and invasion in an integrin alpha-6/beta-1-dependent manner through activation of Rac1. Osteostatin is a potent inhibitor of osteoclastic bone resorption.
Alternative names
PTHRP, PTHLH, Parathyroid hormone-related protein, PTH-rP, PTHrP, Parathyroid hormone-like protein, PLP
Recommended products
Rabbit Polyclonal PTHLH antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PTHLH aa 1 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human PTHLH aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P12272
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Parathyroid hormone-like hormone (PTHLH) also known as PTHrP is a protein made by the PTHLH gene. It weighs around 17 kDa and its expression occurs in many tissues including the bone skin and developing structures during embryogenesis. PTHLH interacts with parathyroid hormone receptors offering actions similar to parathyroid hormone (PTH). This interaction helps to extend its role beyond classical calcium homeostasis to include other functions such as growth and developmental processes.
- Biological function summary
PTHLH plays a role in cartilage development and endochondral bone formation. It functions as a significant regulator of cellular proliferation and differentiation especially in chondrocytes and osteoblasts. PTHLH can also act as part of multiprotein complexes that influence transcription and intracellular signaling. Furthermore it possesses diverse roles including smooth muscle contraction mammary gland development and cellular differentiation inhibition.
- Pathways
Alongside related proteins like Indian Hedgehog (IHH) PTHLH resides in pathways critical for skeletal development and homeostasis. Notably the PTHLH/PTHR1 signaling pathway regulates chondrocyte maturation during bone growth. This pathway intersects with the Hedgehog pathway to form a regulatory loop essential for maintaining the growth plate's development and maturation. PTHLH also influences the Wnt signaling pathway associated with bone density and health.
- Associated diseases and disorders
PTHLH has connections with certain cancers specifically breast cancer and hypercalcemia of malignancy. In cancerous tissues PTHLH can be overexpressed leading to increased osteoclastic bone resorption contributing to cancer-associated conditions. Additionally PTHLH overactivity links it to familial benign hypercalcemia through its interactions with PTH and its receptors. Understanding these associations could offer therapeutic targets for treatment.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTHLH antibody (ab224503)
Paraffin-embedded human breast tissue stained for PTHLH using ab224503 at 1/50 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-PTHLH antibody (ab224503)
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for PTHLH (green) using ab224503 at 4 μg/ml in ICC/IF.
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