Rabbit Recombinant Monoclonal PTHLH antibody. Carrier free. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-PTHLH antibody [EPR26606-236] - BSA and Azide free (AB314119), expandable thumbnail](https://content.abcam.com/products/images/pthlh-antibody-epr26606-236-bsa-and-azide-free-ab314119--western-blot-img419975.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PTHLH antibody [EPR26606-236] - BSA and Azide free (AB314119)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | Flow Cyt (Intra) | IP | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Neuroendocrine peptide which is a critical regulator of cellular and organ growth, development, migration, differentiation and survival and of epithelial calcium ion transport. Regulates endochondral bone development and epithelial-mesenchymal interactions during the formation of the mammary glands and teeth. Required for skeletal homeostasis. Promotes mammary mesenchyme differentiation and bud outgrowth by modulating mesenchymal cell responsiveness to BMPs. Upregulates BMPR1A expression in the mammary mesenchyme and this increases the sensitivity of these cells to BMPs and allows them to respond to BMP4 in a paracrine and/or autocrine fashion. BMP4 signaling in the mesenchyme, in turn, triggers epithelial outgrowth and augments MSX2 expression, which causes the mammary mesenchyme to inhibit hair follicle formation within the nipple sheath (By similarity). Promotes colon cancer cell migration and invasion in an integrin alpha-6/beta-1-dependent manner through activation of Rac1.Osteostatin is a potent inhibitor of osteoclastic bone resorption.
Alternative names
Parathyroid hormone-related protein, PTH-rP, PTHrP, Parathyroid hormone-like protein, PLP, PTHRP, PTHLH
Recommended products
Rabbit Recombinant Monoclonal PTHLH antibody. Carrier free. Suitable for WB and reacts with Human samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR26606-236
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
ab314119 is the carrier-free version of Anti-PTHLH antibody [EPR26606-236] ab314118.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Parathyroid hormone-like hormone (PTHLH) also known as PTHrP is a protein made by the PTHLH gene. It weighs around 17 kDa and its expression occurs in many tissues including the bone skin and developing structures during embryogenesis. PTHLH interacts with parathyroid hormone receptors offering actions similar to parathyroid hormone (PTH). This interaction helps to extend its role beyond classical calcium homeostasis to include other functions such as growth and developmental processes.
- Biological function summary
PTHLH plays a role in cartilage development and endochondral bone formation. It functions as a significant regulator of cellular proliferation and differentiation especially in chondrocytes and osteoblasts. PTHLH can also act as part of multiprotein complexes that influence transcription and intracellular signaling. Furthermore it possesses diverse roles including smooth muscle contraction mammary gland development and cellular differentiation inhibition.
- Pathways
Alongside related proteins like Indian Hedgehog (IHH) PTHLH resides in pathways critical for skeletal development and homeostasis. Notably the PTHLH/PTHR1 signaling pathway regulates chondrocyte maturation during bone growth. This pathway intersects with the Hedgehog pathway to form a regulatory loop essential for maintaining the growth plate's development and maturation. PTHLH also influences the Wnt signaling pathway associated with bone density and health.
- Associated diseases and disorders
PTHLH has connections with certain cancers specifically breast cancer and hypercalcemia of malignancy. In cancerous tissues PTHLH can be overexpressed leading to increased osteoclastic bone resorption contributing to cancer-associated conditions. Additionally PTHLH overactivity links it to familial benign hypercalcemia through its interactions with PTH and its receptors. Understanding these associations could offer therapeutic targets for treatment.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-PTHLH antibody [EPR26606-236] - BSA and Azide free (ab314119), expandable thumbnail](https://content.abcam.com/products/images/pthlh-antibody-epr26606-236-bsa-and-azide-free-ab314119--western-blot-img419975.jpg "Western blot - Anti-PTHLH antibody [EPR26606-236] - BSA and Azide free (ab314119)")
Western blot - Anti-PTHLH antibody [EPR26606-236] - BSA and Azide free (ab314119)
This data was developed using Anti-PTHLH antibody [EPR26606-236] ab314118, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: human cerebellum (PMID: 1874927), human spinal cord (PMID: 1874927).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: 3 secondsAll lanes: Western blot - Anti-PTHLH antibody [EPR26606-236] (Anti-PTHLH antibody [EPR26606-236] ab314118) at 1/1000 dilution
Lane 1: Human stomach tissue lysate at 20 µg
Lane 2: Human kidney tissue lysate at 20 µg
Lane 3: Human breast tissue lysate at 20 µg
Lane 4: Human colon cancer tissue lysate at 20 µg
Lane 5: Human cerebellum tissue lysate at 20 µg
Lane 6: Human spinal cord tissue lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 25 kDa
Exposure time: 3s
Downloads
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