Sample Prep & Detection Kits
Conjugation kitsPurification kitsSample preparation kitsChromogen kitsIHC kitsChIP kitsAccessory Reagents & Controls
Accessory reagents & controlsBiochemicals
BiochemicalsProteins and Peptides
Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
Learn about all product ranges with our product overviews.
Featured events
Make new connections at our global events.
Our programs
New Lab Program
Get a head start with our exclusive new lab discount. Enjoy 20% off and free shipping for three months.
New Biotech Program
Just starting out? Get 15% off and free shipping to your lab for six months.
Product promise
Peace of mind that all products perform as stated.
Product reviews
Leave reviews, get rewarded and help your community.
Trial program
Try untested species and applications to earn money off your next order.
Product Insider Program
Be the first to know about our latest product launches - and unlock exclusive offers and discounts.
Goat Polyclonal PTN antibody. C-terminal. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PTN aa 150 to C-terminus.
IgG
Goat
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Liquid
Polyclonal
IHC-P | WB | |
---|---|---|
Human | Tested | Expected |
Mouse | Expected | Tested |
Rat | Predicted | Predicted |
Chicken | Predicted | Predicted |
Cow | Predicted | Predicted |
Pig | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 3.00000-5.00000 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Chicken, Cow, Pig | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 0.10000-0.30000 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Chicken, Cow, Pig | Dilution info - | Notes - |
Secreted growth factor that mediates its signal through cell-surface proteoglycan and non-proteoglycan receptors (PubMed:16814777, PubMed:11278720, PubMed:19141530). Binds cell-surface proteoglycan receptor via their chondroitin sulfate (CS) groups (PubMed:26896299, PubMed:27445335). Thereby regulates many processes like cell proliferation, cell survival, cell growth, cell differentiation and cell migration in several tissues namely neuron and bone (PubMed:1733956, PubMed:1768439, PubMed:11278720, PubMed:19141530, PubMed:27445335, PubMed:30667096, PubMed:19442624). Also plays a role in synaptic plasticity and learning-related behavior by inhibiting long-term synaptic potentiation (By similarity). Binds PTPRZ1, leading to neutralization of the negative charges of the CS chains of PTPRZ1, inducing PTPRZ1 clustering, thereby causing the dimerization and inactivation of its phosphatase activity leading to increased tyrosine phosphorylation of each of the PTPRZ1 substrates like ALK, CTNNB1 or AFAP1L2 in order to activate the PI3K-AKT pathway (PubMed:17681947, PubMed:27445335, PubMed:30667096, PubMed:16814777, PubMed:10706604). Through PTPRZ1 binding controls oligodendrocyte precursor cell differentiation by enhancing the phosphorylation of AFAP1L2 in order to activate the PI3K-AKT pathway (PubMed:27445335, PubMed:30667096). Forms a complex with PTPRZ1 and integrin alpha-V/beta-3 (ITGAV:ITGB3) that stimulates endothelial cell migration through SRC dephosphorylation and activation that consequently leads to ITGB3 'Tyr-773' phosphorylation (PubMed:19141530). In adult hippocampus promotes dendritic arborization, spine development, and functional integration and connectivity of newborn granule neurons through ALK by activating AKT signaling pathway (By similarity). Binds GPC2 and chondroitin sulfate proteoglycans (CSPGs) at the neuron surface, leading to abrogation of binding between PTPRS and CSPGs and neurite outgrowth promotion (By similarity). Binds SDC3 and mediates bone formation by recruiting and attaching osteoblasts/osteoblast precursors to the sites for new bone deposition (By similarity). Binds ALK and promotes cell survival and cell proliferation through MAPK pathway activation (PubMed:11278720). Inhibits proliferation and enhances differentiation of neural stem cells by inhibiting FGF2-induced fibroblast growth factor receptor signaling pathway (By similarity). Mediates regulatory mechanisms in normal hemostasis and in hematopoietic regeneration and in maintaining the balance of myeloid and lymphoid regeneration (By similarity). In addition may play a role in the female reproductive system, auditory response and the progesterone-induced decidualization pathway (By similarity).
Pleiotrophin, PTN, Heparin-binding brain mitogen, Heparin-binding growth factor 8, Heparin-binding growth-associated molecule, Heparin-binding neurite outgrowth-promoting factor, Heparin-binding neurite outgrowth-promoting factor 1, Osteoblast-specific factor 1, HBBM, HBGF-8, HB-GAM, HBNF, HBNF-1, OSF-1, NEGF1, HBNF1, PTN
Goat Polyclonal PTN antibody. C-terminal. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PTN aa 150 to C-terminus.
Pleiotrophin, PTN, Heparin-binding brain mitogen, Heparin-binding growth factor 8, Heparin-binding growth-associated molecule, Heparin-binding neurite outgrowth-promoting factor, Heparin-binding neurite outgrowth-promoting factor 1, Osteoblast-specific factor 1, HBBM, HBGF-8, HB-GAM, HBNF, HBNF-1, OSF-1, NEGF1, HBNF1, PTN
IgG
Goat
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Liquid
Polyclonal
Affinity purification Immunogen
ab223674 purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Pleiotrophin functions as a potent mitogen promoting cell growth and angiogenesis. It does not operate as part of a larger protein complex but acts independently to influence cellular behavior. Its interaction with receptor protein tyrosine phosphatase (RPTP) modulates the tyrosine phosphorylation state of other proteins affecting downstream signaling. This interaction facilitates communication between cells and their environments influencing development and tissue maintenance.
Pleiotrophin also known as PTN Ptn or $Ptn is a heparin-binding growth factor with a molecular mass of approximately 18 kDa. It is expressed in various tissues notably in the brain during embryonic development and also in adulthood. PTN plays a fundamental role in cellular proliferation differentiation and survival. As a secreted protein it interacts with cell surface receptors to trigger intracellular signaling pathways.
This protein significantly impacts the PI3K/Akt and MAPK/ERK pathways key regulators of cell survival and proliferation. PTN often collaborates with proteins like Midkine (MK) due to their functional similarities and overlapping receptor targets. These pathways help regulate cellular responses to environmental cues contributing to processes such as tissue repair and regeneration.
The overexpression of pleiotrophin has been linked to the development of cancers such as breast cancer and glioblastoma. PTN's interaction with oncogenic pathways often involving proteins like N-cadherin enhances its relevance in tumor progression and metastasis. Additionally alterations in PTN expression or activity can influence neural disorders highlighting its importance in both oncological and neurological contexts.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Primary incubation was 1 hour.
All lanes: Western blot - Anti-PTN antibody - C-terminal (AB223674) at 0.1 µg/mL
All lanes: mouse fetal brain lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 19 kDa
Observed band size: 18 kDa
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com