Anti-PTN antibody [EP3040(2)]

• WB

Unknown

Western blot - Anti-PTN antibody [EP3040(2)] (AB133517)

All lanes:

Western blot - Anti-PTN antibody [EP3040(2)] (ab133517) at 1/1000 dilution

Lane 1:

Fetal brain lysate at 10 µg

Lane 2:

Fetal cerebellum lysate at 10 µg

Secondary

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Goat-anti-rabbit HRP at 1/2000 dilution

Predicted band size: 19 kDa

Observed band size: 18 kDa

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• WB

Lab

Western blot - Anti-PTN antibody [EP3040(2)] (AB133517)

ab133517 was shown to react with PTN in wild-type U87mg cells in Western blot with loss of signal observed in PTN knockout cell line ab288713. Wild-type U87mg and PTN knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab133517 overnight at 4 °C at a 1/500 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.

These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-PTN antibody [EP3040(2)] (ab133517) at 1/500 dilution

Lane 1:

Wild-type U-87 MG lysate at 25 µg

Lane 2:

PTN knock-out U87mg lysate at 25 µg

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• WB

CiteAb

Western blot - Anti-PTN antibody [EP3040(2)] (AB133517)

PTN western blot using anti-PTN antibody [EP3040(2)] ab133517. Publication image and figure legend from Zhang, X., Zhou, Y., et al., 2019, Oncogenesis, PubMed 31822653.

ab133517 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab133517 please see the product overview.

Binding of miRNAs to MACC1-AS1 promotes cell proliferation.a, b qRT-PCR and western blots indicate that cells overexpressing MACC1-AS1 increased the expression of PTN and c-Myc. **p < 0.01 as determined by Student's t test. c, d qRT-PCR indicate that levels of PTN and c-Myc mRNAs were slightly changed when overexpressing MACC1-AS1 lacking a putative miRNA-384 or miRNA-145 binding site, respectively. n.s : not significant. e HEK-293T cells were transfected with miRNA-384 or miRNA-145 siRNA alone (red colored) or co-transfected with siRNAs and vectors expressing MACC1-AS1. qRT-PCR indicating that the miR-384-5p-mediated PTN or miR-145-3p-mediated c-Myc mRNA reduction could be partially rescued by overexpression of MACC1-AS1. **p < 0.01, *p < 0.05 as determined by one-way ANOVA assays. f MTT assays demonstrate that cells expressing mutant MACC1-AS1 without a miR-384-5p or miR-145-3p binding site reduced cell proliferation, in contrast to the cells expressing MACC1-AS1. *p < 0.05.

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