Anti-PTN antibody [EPR3041] - BSA and Azide free

• IP

Lab

Immunoprecipitation - Anti-PTN antibody [EPR3041] - BSA and Azide free (AB247502)

Immunoprecipitation of PTN in U87mg cells. Lysates were prepared and immunoprecipitation was performed using 2.0 µg of ab79411 pre-coupled to Protein A beads. Samples were then washed and processed for western blot

These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Immunoprecipitation - Anti-PTN antibody [EPR3041] (<a href='/en-us/products/primary-antibodies/ptn-antibody-epr3041-ab79411'>ab79411</a>) at 2 µg

All lanes:

U87mg cells

false

• WB

Supplier Data

Western blot - Anti-PTN antibody [EPR3041] - BSA and Azide free (AB247502)

This data was developed using ab79411, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PTN antibody [EPR3041] (<a href='/en-us/products/primary-antibodies/ptn-antibody-epr3041-ab79411'>ab79411</a>) at 1/1000 dilution

All lanes:

Human brain lysate at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 19 kDa

Observed band size: 19 kDa

false

• WB

Lab

Western blot - Anti-PTN antibody [EPR3041] - BSA and Azide free (AB247502)

ab79411 was shown to react with PTN in wild-type U87mg cells in Western blot with loss of signal observed in PTN knockout cell line ab288713. Wild-type U87mg and PTN knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab79411 overnight at 4 °C at a 1/500 dilution. Blots were incubated with secondary antibodies at 0.2ug/mL before imaging.

These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-PTN antibody [EPR3041] (<a href='/en-us/products/primary-antibodies/ptn-antibody-epr3041-ab79411'>ab79411</a>) at 1/500 dilution

Lanes 1 - 2:

Western blot - Human PTN knockout U-87 MG cell line (<a href='/en-us/products/cell-lines/human-ptn-knockout-u-87-mg-cell-line-ab288713'>ab288713</a>)

Lane 1:

Wild-type U87mg lysate at 20 µg

Lane 2:

PTN Knockout U87mg lysate at 20 µg

Observed band size: 19 kDa

false

• WB

Supplier Data

Western blot - Anti-PTN antibody [EPR3041] - BSA and Azide free (AB247502)

This data was developed using ab79411, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PTN antibody [EPR3041] (<a href='/en-us/products/primary-antibodies/ptn-antibody-epr3041-ab79411'>ab79411</a>) at 1/2000 dilution

Lane 1:

Mouse brain lysate at 20 µg

Lane 2:

Mouse cerebellum lysate at 20 µg

Lane 3:

Rat brain lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 19 kDa

Observed band size: 19 kDa

false