Rabbit Polyclonal PTP-H1 phospho S459 antibody. Suitable for WB and reacts with Mouse samples. Immunogen corresponding to Synthetic Peptide within Human PTPN3 phospho S459.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.5
Constituents: 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.24% HEPES, 0.01% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human PTPN3 phospho S459. The exact immunogen used to generate this antibody is proprietary information. Database link P26045
Reactivity data
Select an application| WB | |
|---|---|
| Mouse | Tested |
| Cow | Predicted |
| Dog | Predicted |
| Primates | Predicted |
| Sheep | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Cow, Dog, Primates | Dilution info - | Notes - |
Target data
Function
May act at junctions between the membrane and the cytoskeleton. Possesses tyrosine phosphatase activity.
Alternative names
PTPH1, PTPN3, Tyrosine-protein phosphatase non-receptor type 3, Protein-tyrosine phosphatase H1, PTP-H1
Recommended products
Rabbit Polyclonal PTP-H1 phospho S459 antibody. Suitable for WB and reacts with Mouse samples. Immunogen corresponding to Synthetic Peptide within Human PTPN3 phospho S459.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.5
Constituents: 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.24% HEPES, 0.01% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human PTPN3 phospho S459. The exact immunogen used to generate this antibody is proprietary information. Database link P26045
- Purification technique
- Affinity purification Immunogen
- Specificity
ab169869 is specific for the 104 kDa PTPN3 protein phosphorylated at Ser459. Immunolabeling is blocked by preadsorption of the antibody with the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Immunolabeling is also completely eliminated by treatment with lambda phosphatase.
- Concentration
- Purification notes
ab169869 is prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PTP-H1 also known as PTPN13 or FAP-1 is a protein tyrosine phosphatase with a mass of approximately 270 kDa. It plays a role in dephosphorylation of proteins acting mechanically to remove phosphate groups from tyrosine residues. This process is fundamental for regulating cellular functions. PTP-H1 is expressed in a variety of tissues including the liver kidney and lungs suggesting its importance in diverse physiological roles.
- Biological function summary
Protein tyrosine phosphatases like PTP-H1 regulate signal transduction pathways by counteracting the action of kinases. PTP-H1 functions in apoptosis possibly by interacting with Fas a death receptor that mediates apoptotic signaling. It is also known to form complexes with other signaling molecules and these complexes influence cancer-related pathways by modulating cell growth and survival.
- Pathways
PTP-H1 regulates the MAPK and PI3K/AKT signaling pathways. These pathways are important for cell division growth and apoptosis. PTP-H1 interacts with proteins such as p130CAS and FAK which play roles in the MAPK pathway. By dephosphorylating specific substrates PTP-H1 affects cellular responses to external stimuli such as growth factors influencing both normal and pathological conditions.
- Associated diseases and disorders
PTP-H1 is associated with cancer and autoimmune diseases. Altered expression or activity of PTP-H1 is observed in colorectal cancer where it may affect tumor progression by altering cellular adhesion and migration. In the context of autoimmune diseases PTP-H1 interacts with Fas implicating it in disorders like systemic lupus erythematosus where dysregulation of apoptotic pathways plays a critical role.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-PTP-H1 (phospho S459) antibody (ab169869)
All lanes: Western blot - Anti-PTP-H1 (phospho S459) antibody (ab169869) at 1/1000 dilution
Lane 1: Mouse testes lysate
Lane 2: Mouse testes lysate incubated in lambda-Ptase (400 units/100ul lysate for 30 min, RT) before being exposed to ab169869
Predicted band size: 104 kDa
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