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AB169869

Anti-PTP-H1 (phospho S459) antibody

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(1 Publication)

Rabbit Polyclonal PTP-H1 phospho S459 antibody. Suitable for WB and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PTPN3 phospho S459.

View Alternative Names

PTPH1, PTPN3, Tyrosine-protein phosphatase non-receptor type 3, Protein-tyrosine phosphatase H1, PTP-H1

1 Images
Western blot - Anti-PTP-H1 (phospho S459) antibody (AB169869)
  • WB

Supplier Data

Western blot - Anti-PTP-H1 (phospho S459) antibody (AB169869)

All lanes:

Western blot - Anti-PTP-H1 (phospho S459) antibody (ab169869) at 1/1000 dilution

Lane 1:

Mouse testes lysate

Lane 2:

Mouse testes lysate incubated in lambda-Ptase (400 units/100ul lysate for 30 min, RT) before being exposed to ab169869

Predicted band size: 104 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB

applications

Immunogen

Synthetic Peptide within Human PTPN3 phospho S459. The exact immunogen used to generate this antibody is proprietary information.

P26045

Specificity

ab169869 is specific for the 104 kDa PTPN3 protein phosphorylated at Ser459. Immunolabeling is blocked by preadsorption of the antibody with the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Immunolabeling is also completely eliminated by treatment with lambda phosphatase.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Primates": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab169869 is prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
Storage buffer
pH: 7.5 Constituents: 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.24% HEPES, 0.01% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PTP-H1 also known as PTPN13 or FAP-1 is a protein tyrosine phosphatase with a mass of approximately 270 kDa. It plays a role in dephosphorylation of proteins acting mechanically to remove phosphate groups from tyrosine residues. This process is fundamental for regulating cellular functions. PTP-H1 is expressed in a variety of tissues including the liver kidney and lungs suggesting its importance in diverse physiological roles.
Biological function summary

Protein tyrosine phosphatases like PTP-H1 regulate signal transduction pathways by counteracting the action of kinases. PTP-H1 functions in apoptosis possibly by interacting with Fas a death receptor that mediates apoptotic signaling. It is also known to form complexes with other signaling molecules and these complexes influence cancer-related pathways by modulating cell growth and survival.

Pathways

PTP-H1 regulates the MAPK and PI3K/AKT signaling pathways. These pathways are important for cell division growth and apoptosis. PTP-H1 interacts with proteins such as p130CAS and FAK which play roles in the MAPK pathway. By dephosphorylating specific substrates PTP-H1 affects cellular responses to external stimuli such as growth factors influencing both normal and pathological conditions.

PTP-H1 is associated with cancer and autoimmune diseases. Altered expression or activity of PTP-H1 is observed in colorectal cancer where it may affect tumor progression by altering cellular adhesion and migration. In the context of autoimmune diseases PTP-H1 interacts with Fas implicating it in disorders like systemic lupus erythematosus where dysregulation of apoptotic pathways plays a critical role.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May act at junctions between the membrane and the cytoskeleton. Possesses tyrosine phosphatase activity.
See full target information PTPN3 phospho S459
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental physiology 106:2235-2247 PubMed34605091

2021

Increased myofilament calcium sensitivity is associated with decreased cardiac troponin I phosphorylation in the diabetic rat heart.

Applications

Unspecified application

Species

Unspecified reactive species

Angela C Greenman,Gary M Diffee,Amelia S Power,Gerard T Wilkins,Olivia M S Gold,Jeffrey R Erickson,James C Baldi
View all publications
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Product promise

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