AB126497

Anti-PTP zeta/ Phosphacan antibody

Name: Anti-PTP zeta/ Phosphacan antibody (ab126497) | Abcam
Brand: Abcam Limited
SKU: AB126497
Price: 535 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(8 Publications)

Rabbit Polyclonal PTP zeta/ Phosphacan antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PTPRZ1 aa 800-1000.

View Alternative Names

HTPZP2, PTPRZ, PTPRZ2, PTPZ, PTPRZ1, Receptor-type tyrosine-protein phosphatase zeta, R-PTP-zeta, Protein-tyrosine phosphatase receptor type Z polypeptide 1, Protein-tyrosine phosphatase receptor type Z polypeptide 2, R-PTP-zeta-2

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-PTP zeta/ Phosphacan antibody (AB126497)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PTP zeta/ Phosphacan antibody (AB126497)

Immunofluorescent staining of Human cell line A-431 shows positivity in vesicles. Recommended concentration of ab126497 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP zeta/ Phosphacan antibody (AB126497)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP zeta/ Phosphacan antibody (AB126497)

ab126497 at 1/50 dilution staining PTP zeta/ Phosphacan in paraffin-embedded Human cerebral cortex tissue by Immunohistochemistry.

Supplier Data

Western blot - Anti-PTP zeta/ Phosphacan antibody (AB126497)

All lanes:

Western blot - Anti-PTP zeta/ Phosphacan antibody (ab126497)

All lanes:

Human brain tissue

Predicted band size: 255 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human PTPRZ1 aa 800-1000. The exact immunogen used to generate this antibody is proprietary information.

P23471

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-4 µg/mL", "ICCIF-species-notes": "Recommend PFA Fixation and Triton X-100 treatment" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PTP zeta also known as Phosphacan is a protein tyrosine phosphatase expressed mainly in the central nervous system. It is a chondroitin sulfate proteoglycan with a molecular weight of approximately 340 kDa. This protein is found on neuronal and glial cell surfaces. PTP zeta plays a role in cell signaling by dephosphorylating specific tyrosine residues on target proteins. Its interaction with ECM molecules and receptors supports cell-cell communication and structural organization within neural tissues.

Biological function summary

In development and recovery of nervous system components PTP zeta plays key roles. It engages in signaling networks that regulate cell adhesion migration and proliferation. Phosphacan shares structures with other receptor-like proteins and interfaces with various ligands indicating its involvement in complex multi-protein assemblies. Interaction with growth factors such as pleiotrophin influences neural cell differentiation and axonal growth.

Pathways

PTP zeta integrates into signaling processes controlling neural plasticity and regeneration. It participates in the PI3K/Akt pathway where it modifies cell survival and growth dynamics. The protein also associates with the MAPK/ERK pathway impacting neuronal differentiation and proliferation. Here it interacts with other proteins like fibroblast growth factor (FGF) enhancing its regulatory influence on cellular functions critical to the CNS.

PTP zeta has connections with conditions like glioblastoma and schizophrenia. Altered expression levels associate it with these pathologies as it significantly influences cell proliferation and migration patterns that could contribute to tumor growth or neurodevelopmental anomalies. PTP zeta interacts with integrin proteins which are implicated in mediating these disease mechanisms through their role in ECM and cell cytoskeleton interactions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Protein tyrosine phosphatase that negatively regulates oligodendrocyte precursor proliferation in the embryonic spinal cord. Required for normal differentiation of the precursor cells into mature, fully myelinating oligodendrocytes. May play a role in protecting oligondendrocytes against apoptosis. May play a role in the establishment of contextual memory, probably via the dephosphorylation of proteins that are part of important signaling cascades (By similarity).

See full target information PTPRZ1

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 6:101914 PubMed39809264

2025

High cellular plasticity state of medulloblastoma local recurrence and distant dissemination.

Applications

Unspecified application

Species

Unspecified reactive species

Hailong Liu,Jing Zhang,Ziwei Wang,Wei Wang,Dongming Han,Xuan Chen,Yu Su,Jiao Zhang,Craig Daniels,Olivier Saulnier,Zeyuan John Wang,Chunyu Gu,Fei Liu,Kaiwen Deng,Dongyang Wang,Zhaoyang Feng,Yahui Zhao,Yifei Jiang,Yu Gao,Zijia Liu,Mingxu Ma,Yanong Li,Zitong Zhao,Hongyu Yuan,Youliang Sun,Yanfeng Shi,Tao Yang,Wenxing Li,Xueling Qi,Zejun Duan,Junping Zhang,Mingshan Zhang,Chunjiang Yu,Wei Jin,Xinguang Yu,Yu Tian,Shuaicheng Li,Chunde Li,Michael D Taylor,Jiankang Li,Yong-Qiang Liu,Xiaoguang Qiu,Tao Jiang

NPJ precision oncology 7:133 PubMed38081923

2023

Single-cell transcriptome sequencing reveals spatial distribution of IL34 cancer-associated fibroblasts in hepatocellular carcinoma tumor microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Ganggang Wang,Zhijie Zhou,Wenzhi Jin,Xin Zhang,Hao Zhang,Xiaoliang Wang

Advanced healthcare materials 11:e2201586 PubMed36047642

2022

Tissue Niche Miniature of Glioblastoma Patient Treated with Nano-Awakeners to Induce Suicide of Cancer Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Seon-Jin Yoon,Sewoom Baek,Seung Eun Yu,Euna Jo,Dongkyu Lee,Jin-Kyoung Shim,Ran Joo Choi,Junseong Park,Ju Hyung Moon,Eui-Hyun Kim,Jong Hee Chang,Jung Bok Lee,Joon-Sang Park,Hak-Joon Sung,Seok-Gu Kang

Nature communications 10:2780 PubMed31239441

2019

Lzts1 controls both neuronal delamination and outer radial glial-like cell generation during mammalian cerebral development.

Applications

Unspecified application

Species

Unspecified reactive species

T Kawaue,A Shitamukai,A Nagasaka,Y Tsunekawa,T Shinoda,K Saito,R Terada,M Bilgic,T Miyata,F Matsuzaki,A Kawaguchi

Cell communication and signaling : CCS 16:92 PubMed30497491

2018

Chemotherapy-driven increases in the CDKN1A/PTN/PTPRZ1 axis promote chemoresistance by activating the NF-κB pathway in breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Huang,Deng-Jie Ouyang,Shi Chang,Mo-Yun Li,Lun Li,Qian-Ying Li,Rong Zeng,Qiong-Yan Zou,Juan Su,Piao Zhao,Lei Pei,Wen-Jun Yi

Cell reports 21:3970-3986 PubMed29281841

2017

Developmental Emergence of Adult Neural Stem Cells as Revealed by Single-Cell Transcriptional Profiling.

Applications

Unspecified application

Species

Unspecified reactive species

Scott A Yuzwa,Michael J Borrett,Brendan T Innes,Anastassia Voronova,Troy Ketela,David R Kaplan,Gary D Bader,Freda D Miller

The Journal of clinical investigation 125:3198-214 PubMed26121749

2015

IL-34 mediates acute kidney injury and worsens subsequent chronic kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jea-Hyun Baek,Rui Zeng,Julia Weinmann-Menke,M Todd Valerius,Yukihiro Wada,Amrendra K Ajay,Marco Colonna,Vicki R Kelley

Schizophrenia research 150:366-72 PubMed24035561

2013

Proteoglycan abnormalities in olfactory epithelium tissue from subjects diagnosed with schizophrenia.

Applications

IHC

Species

Human

Harry Pantazopoulos,Anne Boyer-Boiteau,Eric H Holbrook,Woochan Jang,Chang-Gyu Hahn,Steven E Arnold,Sabina Berretta

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-PTP zeta/ Phosphacan antibody

Immunocytochemistry/ Immunofluorescence - Anti-PTP zeta/ Phosphacan antibody (AB126497)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP zeta/ Phosphacan antibody (AB126497)

Western blot - Anti-PTP zeta/ Phosphacan antibody (AB126497)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Complementary Products

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Primary Antibodies

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