Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] - BSA and Azide free (AB290651)

This data was developed using ab290640, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human astrocytoma tissue labelling PTP zeta/ Phosphacan with ab290640 at 1/2000 (0.259 μg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used. Positive staining on human astrocytoma. The section was incubated with ab290640 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] - BSA and Azide free (AB290651)

This data was developed using ab290640, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human liver tissue labelling PTP zeta/ Phosphacan with ab290640 at 1/2000 (0.259 μg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) . Negative control : no staining on human liver. The section was incubated with ab290640 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) .

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] - BSA and Azide free (AB290651)

This data was developed using ab290640, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labelling PTP zeta/ Phosphacan with ab290640 at 1/2000 (0.259 μg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used. Positive staining on human cerebrum. The section was incubated with ab290640 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] - BSA and Azide free (AB290651)

This data was developed using ab290640, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labelling PTP zeta/ Phosphacan with ab290640 at 1/2000 (0.259 μg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) . Positive staining on rat cerebrum. The section was incubated with ab290640 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) .

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] - BSA and Azide free (AB290651)

This data was developed using ab290640, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labelling PTP zeta/ Phosphacan with ab290640 at 1/2000 (0.259 μg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) . Positive staining on mouse cerebrum. The section was incubated with ab290640 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) .

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• WB

Supplier Data

Western blot - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] - BSA and Azide free (AB290651)

This data was developed using ab289860, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration was 5% NFDM/TBST.

Several bands in the range from 380 to 70 kDa were observed. The profile is consistent with what has been described in the literature (PMID : 18713734).

Negative control : liver, U-87 MG(PMID : 7477903, 23170925).

All lanes:

Western blot - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (<a href='/en-us/products/primary-antibodies/ptp-zeta-phosphacan-antibody-epr26270-86-ab290640'>ab290640</a>) at 1/1000 dilution

Lane 1:

Human cerebellum tissue lysate at 20 µg

Lane 2:

Human liver tissue lysate at 20 µg

Lane 3:

Human spinal cord tissue lysate at 20 µg

Lane 4:

NCI-H69 (human small cell lung cancer ) whole cell lysate at 20 µg

Lane 5:

U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 255 kDa

Observed band size: 380-70 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] - BSA and Azide free (AB290651)

This data was developed using ab289860, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration was 5% NFDM/TBST.

After chondroitinase ABC treatment, several bands in the range from 380 to 70 kDa were observed. The profile is consistent with what has been described in the literature (PMID : 18713734).

Negative control : liver(PMID : 7477903).

All lanes:

Western blot - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (<a href='/en-us/products/primary-antibodies/ptp-zeta-phosphacan-antibody-epr26270-86-ab290640'>ab290640</a>) at 1/1000 dilution

Lane 1:

Mouse P14 brain tissue lysate at 20 µg

Lane 2:

Mouse P14 brain tissue lysate treated with 0.05U chondroitinase ABC for 3h at 37℃ at 20 µg

Lane 3:

Mouse liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 255 kDa

Observed band size: 380-70 kDa

false

Exposure time: 48s

• WB

Supplier Data

Western blot - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] - BSA and Azide free (AB290651)

This data was developed using ab289860, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration was 5% NFDM/TBST.

PTPRZ gene generates three isoforms by alternative splicing : two transmembrane isoforms PTPRZ-A and PTPRZ-B, and one secretory isoform PTPRZ-S which is also known as phosphacan. All three isoforms expressed in the brain are highly glycosylated with chondroitin sulfate. After chondroitinase ABC treatment, several bands in the range from 380 to 70 kDa were observed. The profile is consistent with what has been described in the literature (PMID : 18713734).

Negative control : liver(PMID : 7477903).

All lanes:

Western blot - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (<a href='/en-us/products/primary-antibodies/ptp-zeta-phosphacan-antibody-epr26270-86-ab290640'>ab290640</a>) at 1/1000 dilution

Lane 1:

Rat brain tissue lysate at 20 µg

Lane 2:

Rat brain tissue lysate treated with 0.05U chondroitinase ABC for 3h at 37℃ at 20 µg

Lane 3:

Rat liver tissue lysate at 20 µg

Lane 4:

Rat spinal cord tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 255 kDa

false