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Rabbit Recombinant Monoclonal PTP zeta/ Phosphacan antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (AB302790), expandable thumbnail
  • Immunohistochemistry - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (AB302790), expandable thumbnail
  • Immunohistochemistry - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (AB302790), expandable thumbnail
  • Immunohistochemistry - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (AB302790), expandable thumbnail
  • Immunohistochemistry - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (AB302790), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow Cyt
Human
Tested
Tested
Not recommended
Not recommended
Mouse
Tested
Tested
Not recommended
Not recommended
Rat
Tested
Tested
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

1/100

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/100

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Target data

Function

Protein tyrosine phosphatase that negatively regulates oligodendrocyte precursor proliferation in the embryonic spinal cord. Required for normal differentiation of the precursor cells into mature, fully myelinating oligodendrocytes. May play a role in protecting oligondendrocytes against apoptosis. May play a role in the establishment of contextual memory, probably via the dephosphorylation of proteins that are part of important signaling cascades (By similarity).

Alternative names

HTPZP2, PTPRZ, PTPRZ2, PTPZ, PTPRZ1, Receptor-type tyrosine-protein phosphatase zeta, R-PTP-zeta, Protein-tyrosine phosphatase receptor type Z polypeptide 1, Protein-tyrosine phosphatase receptor type Z polypeptide 2, R-PTP-zeta-2

Recommended products

Rabbit Recombinant Monoclonal PTP zeta/ Phosphacan antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR26271-75

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

PTP zeta also known as Phosphacan is a protein tyrosine phosphatase expressed mainly in the central nervous system. It is a chondroitin sulfate proteoglycan with a molecular weight of approximately 340 kDa. This protein is found on neuronal and glial cell surfaces. PTP zeta plays a role in cell signaling by dephosphorylating specific tyrosine residues on target proteins. Its interaction with ECM molecules and receptors supports cell-cell communication and structural organization within neural tissues.

Biological function summary

In development and recovery of nervous system components PTP zeta plays key roles. It engages in signaling networks that regulate cell adhesion migration and proliferation. Phosphacan shares structures with other receptor-like proteins and interfaces with various ligands indicating its involvement in complex multi-protein assemblies. Interaction with growth factors such as pleiotrophin influences neural cell differentiation and axonal growth.

Pathways

PTP zeta integrates into signaling processes controlling neural plasticity and regeneration. It participates in the PI3K/Akt pathway where it modifies cell survival and growth dynamics. The protein also associates with the MAPK/ERK pathway impacting neuronal differentiation and proliferation. Here it interacts with other proteins like fibroblast growth factor (FGF) enhancing its regulatory influence on cellular functions critical to the CNS.

Associated diseases and disorders

PTP zeta has connections with conditions like glioblastoma and schizophrenia. Altered expression levels associate it with these pathologies as it significantly influences cell proliferation and migration patterns that could contribute to tumor growth or neurodevelopmental anomalies. PTP zeta interacts with integrin proteins which are implicated in mediating these disease mechanisms through their role in ECM and cell cytoskeleton interactions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (ab302790), expandable thumbnail

    Western blot - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (ab302790)

    Blocking and dilution buffer and concentration: 5% NFDM/TBST

    PTP zeta/Phosphacan are highly glycosylated with chondroitin sulfate. The molecular weight observed is consistent with what has been described in the literature (PMID:18713734).

    Negative control: liver, lung (PMID:7477903).

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    All lanes: Western blot - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (ab302790) at 1/1000 dilution

    Lane 1: Rat spinal cord tissue lysate at 50 µg

    Lane 2: Rat liver tissue lysate at 50 µg

    Lane 3: Rat lung tissue lysate at 50 µg

    Lane 4: Human cerebellum tissue lysate at 50 µg

    Lane 5: Human spinal cord tissue lysate at 50 µg

    Lane 6: Human liver tissue lysate at 50 µg

    Lane 7: Human lung tissue lysate at 50 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 255 kDa

    Observed band size: 500 kDa, 380 kDa, 300 kDa, 250 kDa, 180 kDa, 90 kDa

    Exposure time: 10s

  • Immunohistochemistry - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (ab302790), expandable thumbnail

    Immunohistochemistry - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (ab302790)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling PTP zeta/ Phosphacan with ab302790 at 1/100 (4.95 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Negative control: No staining on mouse liver.The section was incubated with ab302790 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (ab302790), expandable thumbnail

    Immunohistochemistry - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (ab302790)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling PTP zeta/ Phosphacan with ab302790 at 1/100 (4.95 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Positive staining on human cerebrum.The section was incubated with ab302790 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (ab302790), expandable thumbnail

    Immunohistochemistry - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (ab302790)

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling PTP zeta/ Phosphacan with ab302790 at 1/100 (4.95 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Positive staining on rat cerebrum.The section was incubated with ab302790 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (ab302790), expandable thumbnail

    Immunohistochemistry - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (ab302790)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling PTP zeta/ Phosphacan with ab302790 at 1/100 (4.95 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Positive staining on mouse cerebrum (PMID: 26277687).The section was incubated with ab302790 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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