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AB302790

Anti-PTP zeta/ Phosphacan antibody [EPR26271-75]

  • BOND RX™ Validated
  • 20ul selling size
  • RabMAb
  • Recombinant
  • What is this?

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Rabbit Recombinant Monoclonal PTP zeta/ Phosphacan antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.

View Alternative Names

HTPZP2, PTPRZ, PTPRZ2, PTPZ, PTPRZ1, Receptor-type tyrosine-protein phosphatase zeta, R-PTP-zeta, Protein-tyrosine phosphatase receptor type Z polypeptide 1, Protein-tyrosine phosphatase receptor type Z polypeptide 2, R-PTP-zeta-2

7 Images
Western blot - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (AB302790)
  • WB

Supplier Data

Western blot - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (AB302790)

Blocking and dilution buffer and concentration : 5% NFDM/TBST

PTP zeta/Phosphacan are highly glycosylated with chondroitin sulfate. The molecular weight observed is consistent with what has been described in the literature (PMID : 18713734).

Negative control : liver, lung (PMID : 7477903).

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

All lanes:

Western blot - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (ab302790) at 1/1000 dilution

Lane 1:

Rat spinal cord tissue lysate at 50 µg

Lane 2:

Rat liver tissue lysate at 50 µg

Lane 3:

Rat lung tissue lysate at 50 µg

Lane 4:

Human cerebellum tissue lysate at 50 µg

Lane 5:

Human spinal cord tissue lysate at 50 µg

Lane 6:

Human liver tissue lysate at 50 µg

Lane 7:

Human lung tissue lysate at 50 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/1000 dilution

Predicted band size: 255 kDa

Observed band size: 500 kDa,380 kDa,300 kDa,250 kDa,180 kDa,90 kDa

true

Exposure time: 10s

Western blot - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (AB302790)
  • WB

Supplier Data

Western blot - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (AB302790)

Blocking / Diluting buffer and concentration : 5% NFDM/TBST

PTP zeta/ Phosphacan are highly glycosylated with chondroitin sulfate. The molecular weight observed is consistent with what has been described in the literature (PMID : 18713734).

Negative controls : liver, lung (PMID : 7477903)

All lanes:

Western blot - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (ab302790) at 1/1000 dilution

Lane 1:

Rat cerebellum tissue lysate at 40 µg

Lane 2:

Rat hypothalamus tissue lysate at 40 µg

Lane 3:

Mouse hippocampus tissue lysate at 40 µg

Lane 4:

Mouse cerebellum tissue lysate at 40 µg

Lane 5:

Mouse hypothalamus tissue lysate at 40 µg

Lane 6:

Mouse liver tissue lysate, at 40 µg

Lane 7:

Mouse lung tissue lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 255 kDa

Observed band size: 500 kDa

false

Exposure time: 48s

Western blot - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (AB302790)
  • WB

Supplier Data

Western blot - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (AB302790)

Blocking / Diluting buffer and concentration : 5% NFDM/TBST

PTPRZ gene generates three isoforms by alternative splicing : two transmembrane isoforms PTPRZ-A and PTPRZ-B, and one secretory isoform PTPRZ-S which is also known as phosphacan. All three isoforms expressed in the brain are highly glycosylated with chondroitin sulfate. After chondroitinase ABC treatment, several bands in the range from 380 to 90 kDa were observed. The molecular weight observed is consistent with what has been described in the literature (PMID : 18713734).

All lanes:

Western blot - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (ab302790) at 1/1000 dilution

Lane 1:

Untreated mouse P14 brain tissue lysate at 30 µg

Lane 2:

Mouse P14 brain tissue lysate treated with chondroitinase ABC at 30 µg

Lane 3:

Untreated rat brain tissue lysate at 30 µg

Lane 4:

Rat brain tissue lysate treated with chondroitinase ABC at 30 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 255 kDa

Observed band size: 500 kDa,380 kDa

false

Exposure time: 92s

Immunohistochemistry - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (AB302790)
  • IHC

Supplier Data

Immunohistochemistry - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (AB302790)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling PTP zeta/ Phosphacan with ab302790 at 1/100 (4.95 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Negative control : No staining on mouse liver.The section was incubated with ab302790 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (AB302790)
  • IHC

Supplier Data

Immunohistochemistry - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (AB302790)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling PTP zeta/ Phosphacan with ab302790 at 1/100 (4.95 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Positive staining on mouse cerebrum (PMID : 26277687).The section was incubated with ab302790 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (AB302790)
  • IHC

Supplier Data

Immunohistochemistry - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (AB302790)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling PTP zeta/ Phosphacan with ab302790 at 1/100 (4.95 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Positive staining on rat cerebrum.The section was incubated with ab302790 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (AB302790)
  • IHC

Supplier Data

Immunohistochemistry - Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] (AB302790)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling PTP zeta/ Phosphacan with ab302790 at 1/100 (4.95 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Positive staining on human cerebrum.The section was incubated with ab302790 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Carrier free

    Anti-PTP zeta/ Phosphacan antibody [EPR26271-75] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26271-75

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PTP zeta also known as Phosphacan is a protein tyrosine phosphatase expressed mainly in the central nervous system. It is a chondroitin sulfate proteoglycan with a molecular weight of approximately 340 kDa. This protein is found on neuronal and glial cell surfaces. PTP zeta plays a role in cell signaling by dephosphorylating specific tyrosine residues on target proteins. Its interaction with ECM molecules and receptors supports cell-cell communication and structural organization within neural tissues.
Biological function summary

In development and recovery of nervous system components PTP zeta plays key roles. It engages in signaling networks that regulate cell adhesion migration and proliferation. Phosphacan shares structures with other receptor-like proteins and interfaces with various ligands indicating its involvement in complex multi-protein assemblies. Interaction with growth factors such as pleiotrophin influences neural cell differentiation and axonal growth.

Pathways

PTP zeta integrates into signaling processes controlling neural plasticity and regeneration. It participates in the PI3K/Akt pathway where it modifies cell survival and growth dynamics. The protein also associates with the MAPK/ERK pathway impacting neuronal differentiation and proliferation. Here it interacts with other proteins like fibroblast growth factor (FGF) enhancing its regulatory influence on cellular functions critical to the CNS.

PTP zeta has connections with conditions like glioblastoma and schizophrenia. Altered expression levels associate it with these pathologies as it significantly influences cell proliferation and migration patterns that could contribute to tumor growth or neurodevelopmental anomalies. PTP zeta interacts with integrin proteins which are implicated in mediating these disease mechanisms through their role in ECM and cell cytoskeleton interactions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein tyrosine phosphatase that negatively regulates oligodendrocyte precursor proliferation in the embryonic spinal cord. Required for normal differentiation of the precursor cells into mature, fully myelinating oligodendrocytes. May play a role in protecting oligondendrocytes against apoptosis. May play a role in the establishment of contextual memory, probably via the dephosphorylation of proteins that are part of important signaling cascades (By similarity).
See full target information PTPRZ1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com