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AB201974

Anti-PTP1B antibody [4F8F11]

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(8 Publications)

Mouse Monoclonal PTP1B antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PTPN1 aa 1-250.

View Alternative Names

PTP1B, PTPN1, Tyrosine-protein phosphatase non-receptor type 1, Protein-tyrosine phosphatase 1B, PTP-1B

8 Images
Flow Cytometry - Anti-PTP1B antibody [4F8F11] (AB201974)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-PTP1B antibody [4F8F11] (AB201974)

Flow cytometric analysis of Jurkat cells labeling PTP1B with ab201974 at 1/200 dilution (green) compared to a negative control (red).

Immunocytochemistry/ Immunofluorescence - Anti-PTP1B antibody [4F8F11] (AB201974)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PTP1B antibody [4F8F11] (AB201974)

Immunofluorescent analysis of HeLa cells labeling PTP1B with ab201974 at 1/200 dilution (green). Blue : DRAQ5 fluorescent DNA dye. Red : Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP1B antibody [4F8F11] (AB201974)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP1B antibody [4F8F11] (AB201974)

Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissue labeling PTP1B with ab201974 at 1/200 dilution folowed by DAB staining.

Western blot - Anti-PTP1B antibody [4F8F11] (AB201974)
  • WB

Supplier Data

Western blot - Anti-PTP1B antibody [4F8F11] (AB201974)

Expected MWt is 50 kDa.

All lanes:

Western blot - Anti-PTP1B antibody [4F8F11] (ab201974) at 1/500 dilution

All lanes:

Human PTP1B (aa40-246) recombinant protein

Predicted band size: 50 kDa

false

Western blot - Anti-PTP1B antibody [4F8F11] (AB201974)
  • WB

Lab

Western blot - Anti-PTP1B antibody [4F8F11] (AB201974)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : PTP1B knockout HAP1 cell lysate (20 μg)
Lane 3 : Jurkat cell lysate (20 μg)
Lane 4 : MCF7 cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab201974 observed at 54 kDa. Red - loading control, ab181602, observed at 37 kDa.

ab201974 was shown to specifically react with PTP1B when PTP1B knockout samples were used. Wild-type and PTP1B knockout samples were subjected to SDS-PAGE. ab201974 and ab181602 (loading control to GAPDH) were diluted 1/500 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-PTP1B antibody [4F8F11] (ab201974)

Predicted band size: 50 kDa

false

Western blot - Anti-PTP1B antibody [4F8F11] (AB201974)
  • WB

Supplier Data

Western blot - Anti-PTP1B antibody [4F8F11] (AB201974)

All lanes:

Western blot - Anti-PTP1B antibody [4F8F11] (ab201974) at 1/500 dilution

Lane 1:

Jurkat cell lysate

Lane 2:

MCF7 cell lysate

Predicted band size: 50 kDa

false

Western blot - Anti-PTP1B antibody [4F8F11] (AB201974)
  • WB

Lab

Western blot - Anti-PTP1B antibody [4F8F11] (AB201974)

Lanes 1 - 4 : Merged signal (red and green). Green - ab201974 observed at 51 kDa. Red - loading control ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37 kDa.

ab201974 was shown to react with PTP1B in wild-type HeLa cells in Western blot with loss of signal observed in PTPN1 knockout cell line ab265014 (PTPN1 knockout cell lysate ab257617). Wild-type HeLa and PTPN1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5 % milk in TBS-T (0.1 % Tween®) before incubation with ab201974 and ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4 °C at a 1 in 500 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-PTP1B antibody [4F8F11] (ab201974) at 1/500 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

PTPN1 knockout Hela cell lysate at 20 µg

Lane 2:

Western blot - Human PTPN1 (PTP1B) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-ptpn1-ptp1b-knockout-hela-cell-line-ab265014'>ab265014</a>)

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

MCF7 cell lysate at 20 µg

Predicted band size: 50 kDa

Observed band size: 51 kDa

false

Western blot - Anti-PTP1B antibody [4F8F11] (AB201974)
  • WB

Supplier Data

Western blot - Anti-PTP1B antibody [4F8F11] (AB201974)

All lanes:

Western blot - Anti-PTP1B antibody [4F8F11] (ab201974) at 1/500 dilution

Lane 1:

HEK293 cell lysate

Lane 2:

PTP1B (aa40-246)-hIgGFc transfected HEK293 cell lysate

Predicted band size: 50 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4F8F11

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF, Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human PTPN1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

P18031

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

This product was changed from ascites to supernatant. Lot no's high than GR256355-19 are from Tissue Culture Supernatant
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from tissue culture supernatant.
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Protein tyrosine phosphatase 1B (PTP1B) is an enzyme involved in the dephosphorylation of tyrosine residues in proteins. Known also as PTPN1 this protein has a molecular weight of approximately 50 kDa. PTP1B is widely expressed in tissues but is highly present in the liver muscle and adipose tissue. It plays a critical role in regulating signal transduction pathways by removing phosphate groups from phosphotyrosine residues on target proteins. This action generally serves as a 'turn-off' switch for various signaling pathways.
Biological function summary

PTP1B plays important roles in glucose and lipid metabolism. It is not generally considered a part of a complex functioning instead as a singular enzyme. Its main action involves the negative regulation of the insulin signaling pathway by dephosphorylating the insulin receptor as well as its associated substrates. This regulation helps maintain normal levels of insulin sensitivity influencing cellular glucose uptake and metabolism.

Pathways

PTP1B has important functions in the insulin and leptin signaling pathways. It has a regulatory influence in these pathways affecting key metabolic processes. In the insulin signaling pathway PTP1B closely interacts with the insulin receptor as well as with insulin receptor substrates (IRS). Its dephosphorylating activity can lead to decreased insulin sensitivity. In the leptin pathway PTP1B modulates leptin signaling by dephosphorylating the Janus kinase 2 (JAK2) another protein involved in the control of energy balance and metabolism.

Excessive activity of PTP1B links with disorders such as type 2 diabetes and obesity. Overactivity of PTP1B can lead to insulin resistance contributing to the pathogenesis of type 2 diabetes. In obesity PTP1B may interfere with the normal activity of leptin a hormone critical for regulating energy balance. Its interaction with the insulin receptor and JAK2 makes PTP1B a target for therapeutic interventions aimed at improving insulin sensitivity and energy homeostasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK; inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. May also regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET.
See full target information PTPN1
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 6: PubMed33884962

2021

PD-L1 tumor-intrinsic signaling and its therapeutic implication in triple-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chunhua Chen,Shiheng Li,Junli Xue,Manlong Qi,Xin Liu,Yan Huang,Jinghua Hu,Haidong Dong,Kun Ling

Cytopathology : official journal of the British Society for Clinical Cytology 32:161-168 PubMed33025675

2020

The phosphatase PTPN1/PTP1B is a candidate marker of better chemotherapy response in metastatic high-grade serous carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ben Davidson,Annika Jøntvedt Bock,Arild Holth,Dag Andre Nymoen

Cancer cell international 19:316 PubMed31798345

2019

LncRNA UCA1 facilitated cell growth and invasion through the miR-206/CLOCK axis in glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi Huang,Xuya Zhao,Xiaowen Wu,Lei Xiang,Yingnan Yuan,Shi Zhou,Wenfeng Yu

Cancer cell international 19:275 PubMed31695578

2019

Long non-coding RNA UCA1 promotes breast cancer by upregulating PTP1B expression via inhibiting miR-206.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Li,Qingan Zeng,Jiliang Qiu,Ting Pang,Jianzhong Xian,Xuexia Zhang

Bioscience reports 39: PubMed31048362

2019

miR-206 inhibits cell proliferation, invasion, and migration by down-regulating PTP1B in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Yang,Lunli Zhang,Yuanbin Zhong,Lingling Lai,Xiaopeng Li

Cell reports 26:346-355.e3 PubMed30625317

2019

Leptin Signaling in the Arcuate Nucleus Reduces Insulin's Capacity to Suppress Hepatic Glucose Production in Obese Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Eglantine Balland,Weiyi Chen,Garron T Dodd,Gregory Conductier,Roberto Coppari,Tony Tiganis,Michael A Cowley

Cancer research 79:546-556 PubMed30538120

2018

Differential Subcellular Localization Regulates Oncogenic Signaling by ROS1 Kinase Fusion Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Dana S Neel,David V Allegakoen,Victor Olivas,Manasi K Mayekar,Golzar Hemmati,Nilanjana Chatterjee,Collin M Blakely,Caroline E McCoach,Julia K Rotow,Anh Le,Niki Karachaliou,Rafael Rosell,Jonathan W Riess,Robert Nichols,Robert C Doebele,Trever G Bivona

Nature communications 8:466 PubMed28878211

2017

Assay to visualize specific protein oxidation reveals spatio-temporal regulation of SHP2.

Applications

Unspecified application

Species

Unspecified reactive species

Ryouhei Tsutsumi,Jana Harizanova,Rabea Stockert,Katrin Schröder,Philippe I H Bastiaens,Benjamin G Neel
View all publications
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