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AB88481

Anti-PTP1B antibody

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(3 Publications)

Rabbit Polyclonal PTP1B antibody. Suitable for WB and reacts with Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human PTPN1 aa 100-200.

View Alternative Names

PTP1B, PTPN1, Tyrosine-protein phosphatase non-receptor type 1, Protein-tyrosine phosphatase 1B, PTP-1B

1 Images
Western blot - Anti-PTP1B antibody (AB88481)
  • WB

Unknown

Western blot - Anti-PTP1B antibody (AB88481)

The 50kDa band observed can be specifically blocked using the immunogenic peptide, corresponding to PTP1B amino acids 146-157.

All lanes:

Western blot - Anti-PTP1B antibody (ab88481) at 1/1000 dilution

Lane 1:

Mouse SYF cSrc-transformed cells,untreated

Lane 2:

Mouse SYF cSrc-transformed cells treated withpervanadate (1 mM for 30 minutes)

Predicted band size: 50 kDa

Observed band size: 50 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB

applications

Immunogen

Synthetic Peptide within Human PTPN1 aa 100-200. The exact immunogen used to generate this antibody is proprietary information.

P18031

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Protein tyrosine phosphatase 1B (PTP1B) is an enzyme involved in the dephosphorylation of tyrosine residues in proteins. Known also as PTPN1 this protein has a molecular weight of approximately 50 kDa. PTP1B is widely expressed in tissues but is highly present in the liver muscle and adipose tissue. It plays a critical role in regulating signal transduction pathways by removing phosphate groups from phosphotyrosine residues on target proteins. This action generally serves as a 'turn-off' switch for various signaling pathways.
Biological function summary

PTP1B plays important roles in glucose and lipid metabolism. It is not generally considered a part of a complex functioning instead as a singular enzyme. Its main action involves the negative regulation of the insulin signaling pathway by dephosphorylating the insulin receptor as well as its associated substrates. This regulation helps maintain normal levels of insulin sensitivity influencing cellular glucose uptake and metabolism.

Pathways

PTP1B has important functions in the insulin and leptin signaling pathways. It has a regulatory influence in these pathways affecting key metabolic processes. In the insulin signaling pathway PTP1B closely interacts with the insulin receptor as well as with insulin receptor substrates (IRS). Its dephosphorylating activity can lead to decreased insulin sensitivity. In the leptin pathway PTP1B modulates leptin signaling by dephosphorylating the Janus kinase 2 (JAK2) another protein involved in the control of energy balance and metabolism.

Excessive activity of PTP1B links with disorders such as type 2 diabetes and obesity. Overactivity of PTP1B can lead to insulin resistance contributing to the pathogenesis of type 2 diabetes. In obesity PTP1B may interfere with the normal activity of leptin a hormone critical for regulating energy balance. Its interaction with the insulin receptor and JAK2 makes PTP1B a target for therapeutic interventions aimed at improving insulin sensitivity and energy homeostasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK; inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. May also regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET.
See full target information PTPN1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Life science alliance 5: PubMed35193953

2022

Stress-induced tyrosine phosphorylation of RtcB modulates IRE1 activity and signaling outputs.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandra Papaioannou,Federica Centonze,Alice Metais,Marion Maurel,Luc Negroni,Matías Gonzalez-Quiroz,Sayyed Jalil Mahdizadeh,Gabriella Svensson,Ensieh Zare,Alice Blondel,Albert C Koong,Claudio Hetz,Rémy Pedeux,Michel L Tremblay,Leif A Eriksson,Eric Chevet

Communications biology 3:306 PubMed32533049

2020

Induction of IRAK-M in melanoma induces caspase-3 dependent apoptosis by reducing TRAF6 and calpastatin levels.

Applications

Unspecified application

Species

Unspecified reactive species

Degui Geng,Nicholas Ciavattone,Jackline Joy Lasola,Rojesh Shrestha,Amelia Sanchez,Jitao Guo,Alexandra Vlk,Rania Younis,Lucy Wang,Alex J Brown,Yuji Zhang,Cruz Velasco-Gonzalez,Aik-Choon Tan,Eduardo Davila

PLoS pathogens 14:e1006942 PubMed29570727

2018

miR-126-5p by direct targeting of JNK-interacting protein-2 (JIP-2) plays a key role in Theileria-infected macrophage virulence.

Applications

Unspecified application

Species

Unspecified reactive species

Malak Haidar,Zineb Rchiad,Hifzur Rahman Ansari,Fathia Ben-Rached,Shahin Tajeri,Perle Latre De Late,Gordon Langsley,Arnab Pain
View all publications
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