Rabbit Polyclonal PTP1B antibody. Suitable for WB and reacts with Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human PTPN1 aa 100-200.
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- Life science alliance 5:2022Stress-induced tyrosine phosphorylation of RtcB modulates IRE1 activity and signaling outputs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexandra Papaioannou,Federica Centonze,Alice Metais,Marion Maurel,Luc Negroni,Matías Gonzalez-Quiroz,Sayyed Jalil Mahdizadeh,Gabriella Svensson,Ensieh Zare,Alice Blondel,Albert C Koong,Claudio Hetz,Rémy Pedeux,Michel L Tremblay,Leif A Eriksson,Eric ChevetPubMed 35193953
- Communications biology 3:3062020Induction of IRAK-M in melanoma induces caspase-3 dependent apoptosis by reducing TRAF6 and calpastatin levels.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDegui Geng et. al.PubMed 32533049
- PLoS pathogens 14:e10069422018miR-126-5p by direct targeting of JNK-interacting protein-2 (JIP-2) plays a key role in Theileria-infected macrophage virulence.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMalak Haidar et. al.PubMed 29570727
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human PTPN1 aa 100-200. The exact immunogen used to generate this antibody is proprietary information. Database link P18031
Reactivity data
Select an application| WB | |
|---|---|
| Mouse | Tested |
| Rat | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
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Target data
Function
Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK; inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. May also regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET.
Alternative names
PTP1B, PTPN1, Tyrosine-protein phosphatase non-receptor type 1, Protein-tyrosine phosphatase 1B, PTP-1B
Recommended products
Rabbit Polyclonal PTP1B antibody. Suitable for WB and reacts with Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human PTPN1 aa 100-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human PTPN1 aa 100-200. The exact immunogen used to generate this antibody is proprietary information. Database link P18031
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Protein tyrosine phosphatase 1B (PTP1B) is an enzyme involved in the dephosphorylation of tyrosine residues in proteins. Known also as PTPN1 this protein has a molecular weight of approximately 50 kDa. PTP1B is widely expressed in tissues but is highly present in the liver muscle and adipose tissue. It plays a critical role in regulating signal transduction pathways by removing phosphate groups from phosphotyrosine residues on target proteins. This action generally serves as a 'turn-off' switch for various signaling pathways.
- Biological function summary
PTP1B plays important roles in glucose and lipid metabolism. It is not generally considered a part of a complex functioning instead as a singular enzyme. Its main action involves the negative regulation of the insulin signaling pathway by dephosphorylating the insulin receptor as well as its associated substrates. This regulation helps maintain normal levels of insulin sensitivity influencing cellular glucose uptake and metabolism.
- Pathways
PTP1B has important functions in the insulin and leptin signaling pathways. It has a regulatory influence in these pathways affecting key metabolic processes. In the insulin signaling pathway PTP1B closely interacts with the insulin receptor as well as with insulin receptor substrates (IRS). Its dephosphorylating activity can lead to decreased insulin sensitivity. In the leptin pathway PTP1B modulates leptin signaling by dephosphorylating the Janus kinase 2 (JAK2) another protein involved in the control of energy balance and metabolism.
- Associated diseases and disorders
Excessive activity of PTP1B links with disorders such as type 2 diabetes and obesity. Overactivity of PTP1B can lead to insulin resistance contributing to the pathogenesis of type 2 diabetes. In obesity PTP1B may interfere with the normal activity of leptin a hormone critical for regulating energy balance. Its interaction with the insulin receptor and JAK2 makes PTP1B a target for therapeutic interventions aimed at improving insulin sensitivity and energy homeostasis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-PTP1B antibody (ab88481)
The 50kDa band observed can be specifically blocked using the immunogenic peptide, corresponding to PTP1B amino acids 146-157.
All lanes: Western blot - Anti-PTP1B antibody (ab88481) at 1/1000 dilution
Lane 1: Mouse SYF cSrc-transformed cells,untreated
Lane 2: Mouse SYF cSrc-transformed cells treated withpervanadate (1 mM for 30 minutes)
Predicted band size: 50 kDa
Observed band size: 50 kDa
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