Anti-PTP1B antibody [EPR22468-6] (ab252928)

Rabbit Recombinant Monoclonal PTP1B antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 5 publications.

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Images

Immunoprecipitation - Anti-PTP1B antibody [EPR22468-6] (AB252928), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	Flow Cyt	WB	ICC/IF	IHC-P
Human	Tested	Not recommended	Tested	Not recommended	Tested
Mouse	Expected	Not recommended	Tested	Not recommended	Tested
Rat	Expected	Not recommended	Tested	Not recommended	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/30	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -
Species Human	Dilution info 1/1000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

See full target information PTPN1

Function

Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK; inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. May also regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET.

Alternative names

PTP1B, PTPN1, Tyrosine-protein phosphatase non-receptor type 1, Protein-tyrosine phosphatase 1B, PTP-1B

Recommended products

Rabbit Recombinant Monoclonal PTP1B antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 5 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR22468-6
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Protein tyrosine phosphatase 1B (PTP1B) is an enzyme involved in the dephosphorylation of tyrosine residues in proteins. Known also as PTPN1 this protein has a molecular weight of approximately 50 kDa. PTP1B is widely expressed in tissues but is highly present in the liver muscle and adipose tissue. It plays a critical role in regulating signal transduction pathways by removing phosphate groups from phosphotyrosine residues on target proteins. This action generally serves as a 'turn-off' switch for various signaling pathways.

Biological function summary

PTP1B plays important roles in glucose and lipid metabolism. It is not generally considered a part of a complex functioning instead as a singular enzyme. Its main action involves the negative regulation of the insulin signaling pathway by dephosphorylating the insulin receptor as well as its associated substrates. This regulation helps maintain normal levels of insulin sensitivity influencing cellular glucose uptake and metabolism.

Pathways

PTP1B has important functions in the insulin and leptin signaling pathways. It has a regulatory influence in these pathways affecting key metabolic processes. In the insulin signaling pathway PTP1B closely interacts with the insulin receptor as well as with insulin receptor substrates (IRS). Its dephosphorylating activity can lead to decreased insulin sensitivity. In the leptin pathway PTP1B modulates leptin signaling by dephosphorylating the Janus kinase 2 (JAK2) another protein involved in the control of energy balance and metabolism.

Associated diseases and disorders

Excessive activity of PTP1B links with disorders such as type 2 diabetes and obesity. Overactivity of PTP1B can lead to insulin resistance contributing to the pathogenesis of type 2 diabetes. In obesity PTP1B may interfere with the normal activity of leptin a hormone critical for regulating energy balance. Its interaction with the insulin receptor and JAK2 makes PTP1B a target for therapeutic interventions aimed at improving insulin sensitivity and energy homeostasis.

Product promise

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10 product images

Immunoprecipitation - Anti-PTP1B antibody [EPR22468-6] (ab252928)
PTP1B was immunoprecipitated from 0.35 mg HCT116 (human colorectal carcinoma epithelial cell) whole cell lysate with ab252928 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab252928 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used at 1/5000 dilution.
Lane 1: HCT116 whole cell lysate 10 μg (Input).
Lane 2: ab252928 IP in HCT116 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab252928 in HCT116 whole cell lysate.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 seconds.
The observed MW is consistent with what described in the literature. (PMID:18253097; PMID:11895943; PMID: 19797268).
All lanes: Immunoprecipitation - Anti-PTP1B antibody [EPR22468-6] (ab252928)
Predicted band size: 50 kDa
Observed band size: 36 kDa, 46 kDa, 50 kDa
Western blot - Anti-PTP1B antibody [EPR22468-6] (ab252928)
ab252928 was shown to specifically react with PTP1B in wild-type HAP1 cells as signal was lost in PTP1B knockout cells. Wild-type and PTP1B knockout samples were subjected to SDS-PAGE. ab252928 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/2000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging.
The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.
The expression profile observed is consistent with what has been described in the literature (PMID:18253097; PMID:11895943; PMID: 19797268). The bands below 50kDa may represent truncated forms and cleaved fragments.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lanes 1-3: 3 mins; Lanes 4-6: 15 secs; Lane 7: 3 mins.
All lanes: Western blot - Anti-PTP1B antibody [EPR22468-6] (ab252928) at 1/2000 dilution
Lane 1: HCT116 (human colorectal carcinoma epithelial cell), whole cell lysate at 20 µg
Lane 2: Wild-type HAP1 whole cell lysate at 20 µg
Lane 3: PTP1B knockout HAP1 whole cell lysate at 20 µg
Lane 4: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 5: HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 20 µg
Lane 6: TF-1 (human Erythroleukemia erythroblast), whole cell lysate at 20 µg
Lane 7: A549 (human lung carcinoma epithelial cell), whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 50 kDa
Observed band size: 36 kDa, 46 kDa, 48 kDa, 50 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP1B antibody [EPR22468-6] (ab252928)
Immunohistochemical analysis of paraffin-embedded human breast cancer (panel A) and its adjacent non-cancerous tissue (panel B) labeling PTP1B with ab252928 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Higher cytoplasmic staining in human breast cancer tissue (panel A) while weakly staining in its adjacent non-cancerous tissue (panel B) (PMID: 27465552) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Western blot - Anti-PTP1B antibody [EPR22468-6] (ab252928)
Lanes 1- 2: Merged signal (red and green). Green - ab252928 observed at 50 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.
ab252928 was shown to react with PTP1B in wild-type HeLa cells in western blot. The band observed in knockout cell line Human PTPN1 (PTP1B) knockout HeLa cell line ab265014 (knockout cell lysate Human PTPN1 (PTP1B) knockout HeLa cell lysate ab257617) lane below 50kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HeLa and PTPN1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab252928 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-PTP1B antibody [EPR22468-6] (ab252928) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: PTPN1 knockout HeLa cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Western blot - Anti-PTP1B antibody [EPR22468-6] (ab252928)
Lanes 1- 2: Merged signal (red and green). Green - ab252928 observed at 50 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.
ab252928 was shown to react with PTP1B in wild-type HeLa cells in western blot. The band observed in CRISPR/Cas9 cell line Human PTPN1 (PTP1B) knockout HeLa cell line ab265014 (CRISPR/Cas9 cell lysate Human PTPN1 (PTP1B) knockout HeLa cell lysate ab257617) lane below 50kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HeLa and PTPN1 CRISPR/Cas9 HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab252928 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-PTP1B antibody [EPR22468-6] (ab252928) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: PTPN1 CRISPR/Cas9 edited HeLa cell lysate at 20 µg
Lane 2: Western blot - Human PTPN1 (PTP1B) knockout HeLa cell line (Human PTPN1 (PTP1B) knockout HeLa cell line ab265014)
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Western blot - Anti-PTP1B antibody [EPR22468-6] (ab252928)
The expression profile observed is consistent with what has been described in the literature (PMID:18253097; PMID:11895943; PMID: 19797268). The bands below 50kDa may represent truncated forms and cleaved fragments.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lanes 1-3: 92 secs; Lane 4: 3 mins.
All lanes: Western blot - Anti-PTP1B antibody [EPR22468-6] (ab252928) at 1/1000 dilution
Lane 1: MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 2: SW480 (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 3: Human placenta cell lysate at 20 µg
Lane 4: Human fetal kidney cell lysate at 20 µg
Secondary
Lanes 1 - 3: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Lane 4: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Predicted band size: 50 kDa
Observed band size: 36 kDa, 46 kDa, 50 kDa
Western blot - Anti-PTP1B antibody [EPR22468-6] (ab252928)
The expression profile observed is consistent with what has been described in the literature (PMID:18253097; PMID:11895943; PMID: 19797268). The bands below 50kDa may represent truncated forms and cleaved fragments.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lanes 1-2: 70 secs; Lane 3: 3 mins; Lane 4: 37 secs; Lanes 5-6: 3 mins.
All lanes: Western blot - Anti-PTP1B antibody [EPR22468-6] (ab252928) at 1/1000 dilution
Lane 1: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg
Lane 2: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg
Lane 3: Mouse brain cell lysate at 20 µg
Lane 4: Mouse spleen cell lysate at 20 µg
Lane 5: Rat brain cell lysate at 20 µg
Lane 6: Rat heart cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 50 kDa
Observed band size: 36 kDa, 46 kDa, 48 kDa, 50 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP1B antibody [EPR22468-6] (ab252928)
Immunohistochemical analysis of paraffin-embedded human gastric cancer (panel A) and its adjacent non-cancerous tissue (panel B) labeling PTP1B with ab252928 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Higher cytoplasmic staining in human gastric cancer tissue (panel A) while weakly staining in its adjacent non-cancerous tissue (panel B) (PMID: 21442314) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP1B antibody [EPR22468-6] (ab252928)
Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling PTP1B with ab252928 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in rat spleen (PMID:18716132) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP1B antibody [EPR22468-6] (ab252928)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling PTP1B with ab252928 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in mouse spleen (PMID:18716132) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).