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AB88472

Anti-PTP1B (phospho S50) antibody

4

(1 Review)

|

(2 Publications)

Rabbit Polyclonal PTP1B phospho S50 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PTPN1 phospho S50.

View Alternative Names

PTP1B, PTPN1, Tyrosine-protein phosphatase non-receptor type 1, Protein-tyrosine phosphatase 1B, PTP-1B

1 Images
Western blot - Anti-PTP1B (phospho S50) antibody (AB88472)
  • WB

Unknown

Western blot - Anti-PTP1B (phospho S50) antibody (AB88472)

All lanes:

Western blot - Anti-PTP1B (phospho S50) antibody (ab88472) at 1/1000 dilution

Lane 1:

lysate from untreated Jurkat cells

Lane 2:

lysate from Jurkat cells treated with calyculin A (100 nM for 30 minutes)

Predicted band size: 50 kDa

Observed band size: 50 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human PTPN1 phospho S50. The exact immunogen used to generate this antibody is proprietary information.

P18031

Specificity

ab88472 reactivity is observed as a weak singlet in control (untreated) cells and is not observed after lambda phosphatase treatment.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Protein tyrosine phosphatase 1B (PTP1B) is an enzyme involved in the dephosphorylation of tyrosine residues in proteins. Known also as PTPN1 this protein has a molecular weight of approximately 50 kDa. PTP1B is widely expressed in tissues but is highly present in the liver muscle and adipose tissue. It plays a critical role in regulating signal transduction pathways by removing phosphate groups from phosphotyrosine residues on target proteins. This action generally serves as a 'turn-off' switch for various signaling pathways.
Biological function summary

PTP1B plays important roles in glucose and lipid metabolism. It is not generally considered a part of a complex functioning instead as a singular enzyme. Its main action involves the negative regulation of the insulin signaling pathway by dephosphorylating the insulin receptor as well as its associated substrates. This regulation helps maintain normal levels of insulin sensitivity influencing cellular glucose uptake and metabolism.

Pathways

PTP1B has important functions in the insulin and leptin signaling pathways. It has a regulatory influence in these pathways affecting key metabolic processes. In the insulin signaling pathway PTP1B closely interacts with the insulin receptor as well as with insulin receptor substrates (IRS). Its dephosphorylating activity can lead to decreased insulin sensitivity. In the leptin pathway PTP1B modulates leptin signaling by dephosphorylating the Janus kinase 2 (JAK2) another protein involved in the control of energy balance and metabolism.

Excessive activity of PTP1B links with disorders such as type 2 diabetes and obesity. Overactivity of PTP1B can lead to insulin resistance contributing to the pathogenesis of type 2 diabetes. In obesity PTP1B may interfere with the normal activity of leptin a hormone critical for regulating energy balance. Its interaction with the insulin receptor and JAK2 makes PTP1B a target for therapeutic interventions aimed at improving insulin sensitivity and energy homeostasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK; inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. May also regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET.
See full target information PTPN1 phospho S50
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of cell biology 221: PubMed35171230

2022

Pluripotency exit is guided by the Peln1-mediated disruption of intrachromosomal architecture.

Applications

Unspecified application

Species

Unspecified reactive species

Yichen Wang,Lin Jia,Cong Wang,Zhonghua Du,Shilin Zhang,Lei Zhou,Xue Wen,Hui Li,Huiling Chen,Yuanyuan Nie,Dan Li,Shanshan Liu,Daniela Salgado Figueroa,Ferhat Ay,Wei Xu,Songling Zhang,Wei Li,Jiuwei Cui,Andrew R Hoffman,Hui Guo,Ji-Fan Hu

Communications biology 3:306 PubMed32533049

2020

Induction of IRAK-M in melanoma induces caspase-3 dependent apoptosis by reducing TRAF6 and calpastatin levels.

Applications

Unspecified application

Species

Unspecified reactive species

Degui Geng,Nicholas Ciavattone,Jackline Joy Lasola,Rojesh Shrestha,Amelia Sanchez,Jitao Guo,Alexandra Vlk,Rania Younis,Lucy Wang,Alex J Brown,Yuji Zhang,Cruz Velasco-Gonzalez,Aik-Choon Tan,Eduardo Davila
View all publications
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