Anti-PTP1B + TCPTP antibody [EPR6712] - BSA and Azide free

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-PTP1B + TCPTP antibody [EPR6712] - BSA and Azide free (AB248278)

This data was developed using ab129070, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of MOLT-4 (human lymphoblastic leukemia T lymphoblast) cell line fixed with 4% paraformaldehyde and permeabilized with 90% methanol labeling TCPTP with ab129070 at 1/200 dilution (red). This is compared with a Rabbit monoclonal IgG – Isotype control (ab172730) (black) and a unalbelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-rabbit IgG (Alexa Fluor^®488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

• IP

Lab

Immunoprecipitation - Anti-PTP1B + TCPTP antibody [EPR6712] - BSA and Azide free (AB248278)

This data was developed using ab129070, the same antibody clone in a different buffer formulation.

Lane 1 (input) : MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate, 10μg

Lane 2 (+) : MOLT-4 whole cell lysate

Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab129070 in MOLT-4 whole cell lysate.

ab129070 immunoprecipitating TCPTP in MOLT-4 whole cell lysate. Capture antibody was used at a 1 : 20 dilution (0.6μg in 0.35mg lysates). For western blotting, primary antibody used was ab129070 at 1 : 1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 1 second.

All lanes:

Immunoprecipitation - Anti-PTP1B + TCPTP antibody [EPR6712] (<a href='/en-us/products/primary-antibodies/ptp1b-tcptp-antibody-epr6712-ab129070'>ab129070</a>)

false

Exposure time: 1s

• WB

Unknown

Western blot - Anti-PTP1B + TCPTP antibody [EPR6712] - BSA and Azide free (AB248278)

This data was developed using ab129070, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PTP1B + TCPTP antibody [EPR6712] (<a href='/en-us/products/primary-antibodies/ptp1b-tcptp-antibody-epr6712-ab129070'>ab129070</a>) at 1/1000 dilution

Lane 1:

HL60 cell lysate at 10 µg

Lane 2:

NIH/3T3 cell lysate at 10 µg

Lane 3:

Mouse placenta tissue lysate at 10 µg

Lane 4:

Mouse thymus tissue lysate at 10 µg

Lane 5:

MOLT4 cell lysate at 10 µg

Lane 6:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Observed band size: 45-48 kDa

false

• WB

Lab

Western blot - Anti-PTP1B + TCPTP antibody [EPR6712] - BSA and Azide free (AB248278)

This data was developed using ab129070, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

N-GST tagged full length recombinant human TCPTP protein (Cat# : ab268903)

N-GST tagged Full length Recombinant human PTP1B protein (Cat# : ab268897)

All lanes:

Western blot - Anti-PTP1B + TCPTP antibody [EPR6712] (<a href='/en-us/products/primary-antibodies/ptp1b-tcptp-antibody-epr6712-ab129070'>ab129070</a>) at 1/1000 dilution

Lanes 1 and 3:

N-GST tagged full length recombinant human TCPTP (PTPN2) protein 10ng

Lanes 2 and 4:

N-GST tagged full length recombinant human PTP1B (PTPN1) protein 10ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 68-75 kDa

false

Exposure time: 5s