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Rabbit Recombinant Monoclonal PTP1B antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human, Mouse samples.

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Images

Western blot - Anti-PTP1B + TCPTP antibody [EPR6712] - BSA and Azide free (AB248278), expandable thumbnail
  • Western blot - Anti-PTP1B + TCPTP antibody [EPR6712] - BSA and Azide free (AB248278), expandable thumbnail
  • Immunoprecipitation - Anti-PTP1B + TCPTP antibody [EPR6712] - BSA and Azide free (AB248278), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-PTP1B + TCPTP antibody [EPR6712] - BSA and Azide free (AB248278), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIHC-PIPWBFlow Cyt (Intra)
Human
Not recommended
Not recommended
Tested
Tested
Tested
Mouse
Not recommended
Not recommended
Expected
Tested
Expected
Rat
Not recommended
Not recommended
Predicted
Predicted
Predicted

Not recommended
Not recommended

Species
Rat, Human, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human, Mouse
Dilution info
-
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK; inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. May also regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET.

Additional Targets

PTPN2

Alternative names

PTP1B, PTPN1, Tyrosine-protein phosphatase non-receptor type 1, Protein-tyrosine phosphatase 1B, PTP-1B

Recommended products

Rabbit Recombinant Monoclonal PTP1B antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR6712
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab248278 is the carrier-free version of Anti-PTP1B + TCPTP antibody [EPR6712] ab129070.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PTP1B also known as protein tyrosine phosphatase non-receptor type 1 mechanically functions as an important regulator of signaling pathways by removing phosphate groups from tyrosine residues on target proteins. It has an approximate mass of 50 kDa and is widely expressed in various tissues including the liver muscle and both central and peripheral nervous systems. TCPTP another name for this protein has similar enzymatic properties and serves overlapping and distinct functions in cellular regulation.

Biological function summary

Protein tyrosine phosphatase 1B (PTP1B) plays a critical role in controlling cell growth differentiation and metabolism. It influences the insulin signaling pathway affecting glucose homeostasis and energy utilization. PTP1B also engages in immune cell signaling and modulation of inflammatory responses. While typically studied independently this phosphatase can also form part of larger molecular complexes that impact broader signaling environments.

Pathways

Protein tyrosine phosphatase 1B acts as a central node in the insulin and leptin signaling pathways. These pathways significantly influence metabolic processes and energy balance with PTP1B modulating key interactions with insulin receptors and leptin receptors. It closely interacts with proteins such as IRS1 and IRS2 which play substantial roles in transmitting signals downstream of insulin action impacting cellular uptake of glucose and lipid metabolism.

Associated diseases and disorders

Improper regulation of PTP1B links to type 2 diabetes and obesity due to its involvement in insulin resistance and energy imbalance. Altered PTP1B activity affects insulin receptor function contributing to the pathophysiology of these metabolic disorders. Additionally in relation to obesity PTP1B modulates leptin receptor signaling an important aspect in energy expenditure and weight regulation. These connections make it a target for potential therapeutic interventions focused on metabolic diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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