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AB281686

Anti-PTP4A3/PRL-R antibody [EPR24449-3] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal PTP4A3/PRL-R antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

PRL3, PTP4A3, Protein tyrosine phosphatase type IVA 3, PRL-R, Protein-tyrosine phosphatase 4a3, Protein-tyrosine phosphatase of regenerating liver 3, PRL-3

1 Images
Sandwich ELISA - Anti-PTP4A3/PRL-R antibody [EPR24449-3] - BSA and Azide free (Detector) (AB281686)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-PTP4A3/PRL-R antibody [EPR24449-3] - BSA and Azide free (Detector) (AB281686)

Example of Human PTP4A3/PRL-R standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24449-3

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Product details

ab281686 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human PTP4A3/PRL-R. The recommended pair for sandwich ELISA is:
Capture: ab281685, Human PTP4A3/PRL-R Capture Antibody (unconjugated)
Detector: ab281686, Human PTP4A3/PRL-R Detector Antibody (unconjugated)
The reference range value is 254-15,000 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PTP4A3 also known as PRL-R is a protein tyrosine phosphatase with a molecular mass of approximately 19 kDa. This protein is expressed in various tissues but has high levels in cancerous cells. It has a role in the dephosphorylation of substrates impacting cell processes like proliferation and motility. PTP4A3 localizes to the cytoplasm and its enzymatic activity can influence cellular dynamics particularly in rapidly dividing cells.
Biological function summary

PTP4A3 affects cellular growth and division as part of the signaling network. It is involved in the intracellular control of the phosphatase complex and regulates other protein interactions. PTP4A3 influences cell migration and invasion particularly through interactions with structural proteins. As a phosphatase it removes phosphate groups influencing the phosphorylation status of other proteins and altering their functions in the signaling pathways.

Pathways

PTP4A3 takes part in processes like the MAPK and PI3K/AKT signaling pathways. These pathways are critical for cell survival proliferation and differentiation. Within these pathways PTP4A3 modulates interactions with proteins like ERK1/2 and AKT which are important components of the pathways. The phosphatase activity facilitates crosstalk and integration of signals maintaining cellular homeostasis.

Aberrant PTP4A3 expression connects to cancer particularly in metastatic progression. Elevated levels of PTP4A3 correlate with aggressive tumor behavior and poor prognosis. In addition PTP4A3 affects chronic inflammatory disorders through its actions on signaling pathways. It impacts epithelial to mesenchymal transition (EMT) processes linking to proteins like MMPs which play a role in cancer metastasis and inflammation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein tyrosine phosphatase which stimulates progression from G1 into S phase during mitosis. Enhances cell proliferation, cell motility and invasive activity, and promotes cancer metastasis. May be involved in the progression of cardiac hypertrophy by inhibiting intracellular calcium mobilization in response to angiotensin II.
See full target information PTP4A3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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