AB224081

Anti-PTPIP51 antibody

Name: Anti-PTPIP51 antibody (ab224081) | Abcam
Brand: Abcam Limited
SKU: AB224081

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(3 Publications)

Rabbit Polyclonal PTPIP51 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RMDN3 aa 150-350.

View Alternative Names

FAM82A2, FAM82C, PTPIP51, hucep-10, UNQ3122/PRO10274, RMDN3, Regulator of microtubule dynamics protein 3, RMD-3, hRMD-3, Cerebral protein 10, Protein FAM82A2, Protein FAM82C, Protein tyrosine phosphatase-interacting protein 51, TCPTP-interacting protein 51

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-PTPIP51 antibody (AB224081)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PTPIP51 antibody (AB224081)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for PTPIP51 (green) using ab224081 (4 μg/ml) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTPIP51 antibody (AB224081)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTPIP51 antibody (AB224081)

Paraffin embedded human testis tissue stained for PTPIP51 with ab224081 (1/1000 dilution) in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTPIP51 antibody (AB224081)

Paraffin-embedded human testis tissue staining PTPIP51 with ab224081 at 1/500 dilution in immunohistochemical analysis. Strong cytoplasmic positibity in seminiferous duct cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTPIP51 antibody (AB224081)

Paraffin-embedded human skeletal muscle tissue staining PTPIP51 with ab224081 at 1/500 dilution in immunohistochemical analysis. Weak cytoplasmic positivity in myocytes.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTPIP51 antibody (AB224081)

Paraffin-embedded human skin tissue staining PTPIP51 with ab224081 at 1/500 dilution in immunohistochemical analysis. Moderate to strong cytoplasmic positibity in keratinocytes.

Supplier Data

Western blot - Anti-PTPIP51 antibody (AB224081)

All lanes:

Western blot - Anti-PTPIP51 antibody (ab224081) at 1/250 dilution

All lanes:

Caco-2 (human colorectal adenocarcinoma cell line) cell lysate

Predicted band size: 52 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human RMDN3 aa 150-350. The exact immunogen used to generate this antibody is proprietary information.

Q96TC7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PTPIP51 also known as Protein Tyrosine Phosphatase-Interacting Protein 51 functions at a mechanical level by interacting with protein tyrosine phosphatases and kinases which are key regulators of signal transduction. PTPIP51 has a molecular mass of approximately 51 kDa. The protein is expressed in various tissues including the brain testis and skeletal muscle. Its interactions suggest a role in maintaining cellular function and signaling.

Biological function summary

The PTPIP51 protein plays a role in cellular processes such as apoptosis and cellular differentiation. It is believed to associate with other proteins to form complexes which influence mitochondrial function and cellular energy metabolism. Through these interactions PTPIP51 aids in maintaining cellular homeostasis and regulating cell survival mechanisms.

Pathways

PTPIP51 participates in the regulation of the MAPK/ERK pathway and the insulin signaling pathway. It interacts with proteins such as RAF1 and GRB2 to convey signals from the cell surface to the DNA in the cell nucleus affecting cell division and proliferation. These pathways highlight the protein’s importance in controlling cellular growth and reaction to external stimuli.

PTPIP51 has connections to neurodegenerative disorders like Alzheimer's disease and metabolic conditions such as obesity. In Alzheimer’s alterations in PTPIP51 are linked with the dysregulation of mitochondrial function and it interacts with proteins such as Tau involved in neurofibrillary tangles. In obesity PTPIP51 is connected to insulin resistance engaging with protein partners like IRS1 to influence glucose uptake and metabolism.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in cellular calcium homeostasis regulation. May participate in differentiation and apoptosis of keratinocytes. Overexpression induces apoptosis.

See full target information RMDN3

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

EMBO molecular medicine 17:2409-2437 PubMed40770117

2025

A fetal oncogene NUAK2 is an emerging therapeutic target in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hanhee Jo,Sarah Munoz,Aneesh Dalvi,Wenqi Yang,Elizabeth Morozova,Stacey M Glasgow

Acta neuropathologica communications 12:140 PubMed39198924

2024

A deep phenotyping study in mouse and iPSC models to understand the role of oligodendroglia in optic neuropathy in Wolfram syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

K Ahuja,M Vandenabeele,F Nami,E Lefevere,J Van Hoecke,S Bergmans,M Claes,T Vervliet,K Neyrinck,T Burg,D De Herdt,P Bhaskar,Y Zhu,Z J Looser,J Loncke,W Gsell,M Plaas,P Agostinis,J V Swinnen,L Van Den Bosch,G Bultynck,A S Saab,E Wolfs,Y C Chai,U Himmelreich,C Verfaillie,L Moons,L De Groef

Science advances 6:eaax9093 PubMed32494695

2020

Pharmacological modulation of mitochondrial calcium uniporter controls lung inflammation in cystic fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Alessandro Rimessi,Chiara Pozzato,Lorenzo Carparelli,Alice Rossi,Serena Ranucci,Ida De Fino,Cristina Cigana,Anna Talarico,Mariusz R Wieckowski,Carla M P Ribeiro,Claudio Trapella,Giacomo Rossi,Giulio Cabrini,Alessandra Bragonzi,Paolo Pinton

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PTPIP51 antibody

Immunocytochemistry/ Immunofluorescence - Anti-PTPIP51 antibody (AB224081)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTPIP51 antibody (AB224081)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTPIP51 antibody (AB224081)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTPIP51 antibody (AB224081)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTPIP51 antibody (AB224081)

Western blot - Anti-PTPIP51 antibody (AB224081)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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